Nanotopographic Surfaces with Defined Surface Chemistries from Amyloid Fibril Networks Can Control Cell Attachment

2013 ◽  
Vol 14 (7) ◽  
pp. 2305-2316 ◽  
Author(s):  
Nicholas P. Reynolds ◽  
Katie E. Styan ◽  
Christopher D. Easton ◽  
Yali Li ◽  
Lynne Waddington ◽  
...  
Keyword(s):  
Amyloid Fibril ◽  
Cell Attachment ◽  
Control Cell ◽  
Surface Chemistries

Screening rat mesenchymal stem cell attachment and differentiation on surface chemistries using plasma polymer gradients

2015 ◽  
Vol 11 ◽  
pp. 58-67 ◽  
Author(s):  
Peng-Yuan Wang ◽  
Lauren R. Clements ◽  
Helmut Thissen ◽  
Wei-Bor Tsai ◽  
Nicolas H. Voelcker
Keyword(s):  
Stem Cell ◽  
Mesenchymal Stem Cell ◽  
Cell Attachment ◽  
Plasma Polymer ◽  
Surface Chemistries

Photosensitive chitosan to control cell attachment

2011 ◽  
Vol 361 (1) ◽  
pp. 71-78 ◽  
Author(s):  
Nan Cheng ◽  
Xudong Cao
Keyword(s):  
Cell Attachment ◽  
Control Cell

scholarly journals Biomimetic Topography and Chemistry Control Cell Attachment to Amyloid Fibrils

2015 ◽  
Vol 16 (5) ◽  
pp. 1556-1565 ◽  
Author(s):  
Nicholas P. Reynolds ◽  
Mirren Charnley ◽  
Marie N. Bongiovanni ◽  
Patrick G. Hartley ◽  
Sally L. Gras
Keyword(s):  
Amyloid Fibrils ◽  
Cell Attachment ◽  
Control Cell

Chemical modification of bioinspired superhydrophobic polystyrene surfaces to control cell attachment/proliferation

Soft Matter ◽  
2011 ◽  
Vol 7 (19) ◽  
pp. 8932 ◽  
Author(s):  
Sara M. Oliveira ◽  
Wenlong Song ◽  
Natália M. Alves ◽  
João F. Mano
Keyword(s):  
Chemical Modification ◽  
Cell Attachment ◽  
Control Cell

scholarly journals Development of a modular, biocompatible thiolated gelatin microparticle platform for drug delivery and tissue engineering applications

2021 ◽  
Author(s):  
Hannah A Pearce ◽  
Yu Seon Kim ◽  
Emma Watson ◽  
Kiana Bahrami ◽  
Mollie M Smoak ◽  
...  
Keyword(s):  
Drug Delivery ◽  
Tissue Engineering ◽  
Stem Cell ◽  
Cell Attachment ◽  
Control Cell ◽  
Growth Factor Delivery ◽  
Limited Ability ◽  
The Past ◽  
Modular Platform ◽  
Microparticle Formation

Abstract The field of biomaterials has advanced significantly in the past decade. With the growing need for high-throughput manufacturing and screening, the need for modular materials that enable streamlined fabrication and analysis of tissue engineering and drug delivery schema has emerged. Microparticles are a powerful platform that have demonstrated promise in enabling these technologies without the need to modify a bulk scaffold. This building block paradigm of using microparticles within larger scaffolds to control cell ratios, growth factors and drug release holds promise. Gelatin microparticles (GMPs) are a well-established platform for cell, drug and growth factor delivery. One of the challenges in using GMPs though is the limited ability to modify the gelatin post-fabrication. In the present work, we hypothesized that by thiolating gelatin before microparticle formation, a versatile platform would be created that preserves the cytocompatibility of gelatin, while enabling post-fabrication modification. The thiols were not found to significantly impact the physicochemical properties of the microparticles. Moreover, the thiolated GMPs were demonstrated to be a biocompatible and robust platform for mesenchymal stem cell attachment. Additionally, the thiolated particles were able to be covalently modified with a maleimide-bearing fluorescent dye and a peptide, demonstrating their promise as a modular platform for tissue engineering and drug delivery applications.

Controlling Cell Growth by Nanoparticles

2006 ◽  
Vol 950 ◽  
Author(s):  
Sergiy Zankovych ◽  
Joerg Bossert ◽  
Ines Thiele ◽  
Klaus D. Jandt ◽  
Liga Berzina-Cimdina
Keyword(s):  
Surface Roughness ◽  
Cell Proliferation ◽  
Cell Growth ◽  
Titanium Surface ◽  
Cell Attachment ◽  
Microcontact Printing ◽  
Control Cell ◽  
Superficial Layer ◽  
Titanium Surfaces ◽  
Titanium Substrates

ABSTRACTWe report preliminary results of using nanoparticles to control cell attachment and growth. We present the way to create titanium surfaces with different roughness in a rage between 2 nm and 117 nm by using nanoparticles as a superficial layer and varying the evaporation parameters. We examined cell proliferation on titanium substrates with increased surface roughness compared to smooth titanium surface. We used nanoparticles to create a micrometer-sized lateral layout onto substrates preliminary structured by microcontact printing. We demonstrate controlled cell growth on substrates laterally structured with nanoparticles.

An efficient surface modification using 2-methacryloyloxyethyl phosphorylcholine to control cell attachment via photochemical reaction in a microchannel

Lab on a Chip ◽  
2010 ◽  
Vol 10 (15) ◽  
pp. 1937 ◽  
Author(s):  
Kihoon Jang ◽  
Kae Sato ◽  
Yo Tanaka ◽  
Yan Xu ◽  
Moritoshi Sato ◽  
...  
Keyword(s):  
Surface Modification ◽  
Photochemical Reaction ◽  
Cell Attachment ◽  
Control Cell

scholarly journals Upper critical solution temperature thermo-responsive polymer brushes and a mechanism for controlled cell attachment

2017 ◽  
Vol 5 (25) ◽  
pp. 4926-4933 ◽  
Author(s):  
Xuan Xue ◽  
Lalitha Thiagarajan ◽  
Shwana Braim ◽  
Brian R Saunders ◽  
Kevin M Shakesheff ◽  
...  
Keyword(s):  
Polymer Brushes ◽  
Cell Attachment ◽  
Control Cell ◽  
Solution Temperature ◽  
Critical Solution Temperature ◽  
Upper Critical Solution Temperature ◽  
Responsive Polymer

We report the synthesis of thermo-responsive polymer brushes with Upper Critical Solution Temperature (UCST)-type behaviour on glass to provide a new means to control cell attachment.

scholarly journals Improving cell distribution on 3D additive manufactured scaffolds through engineered seeding media density and viscosity

2019 ◽  
Author(s):  
M. Cámara-Torres ◽  
R. Sinha ◽  
C. Mota ◽  
L. Moroni
Keyword(s):  
Cell Attachment ◽  
Control Cell ◽  
Chemical Properties ◽  
Universal Method ◽  
Cell Distribution ◽  
Geometrical Properties ◽  
Homogeneous Cell ◽  
Static Seeding

AbstractIn order to ensure the long-term in vitro and in vivo functionality of cell-seeded 3D scaffolds, an effective and reliable method to control cell seeding efficiency and distribution is crucial. Static seeding on 3D additive manufactured scaffolds made of synthetic polymers still remains challenging, as it often results in poor cell attachment, high cell sedimentation and non-uniform cell distribution, due to gravity and to the intrinsic macroporosity and surface chemical properties of the scaffolds. In this study, the bio-inert macromolecules dextran and Ficoll were used for the first time as temporary supplements to alter the viscosity and density of the seeding media, respectively, and improve the static seeding output. The addition of these macromolecules drastically reduced the cell sedimentation velocities, allowing for homogeneous cell attachment to the scaffold filaments. Both dextran- and Ficoll-based seeding methods supported human mesenchymal stromal cells viability and osteogenic differentiation post-seeding. Interestingly, the improved cell distribution led to increased matrix production and mineralization compared to scaffolds seeded by conventional static method. These results suggest a simple and universal method for an efficient seeding of 3D additive manufactured scaffolds, independent of their material and geometrical properties, and applicable for bone and various other tissue regeneration.

Immunocytochemical Localization of Amyloid Protein AA in the “Dense Fibrillar Inclusions” of the Reticuloendothelical Cells

1977 ◽  
Vol 35 ◽  
pp. 438-439
Author(s):  
T. Shirahama ◽  
M. Skinner ◽  
A.S. Cohen
Keyword(s):  
Amyloid Fibril ◽  
Close Association ◽  
Gel Filtration ◽  
Fibril Formation ◽  
Amyloid Protein ◽  
Electron Microscopic ◽  
Amyloid Deposits ◽  
Amyloid Fibril Formation ◽  
Electron Microscopic Technique ◽  
Lysosomal System

A1thought the mechanisms of amyloidogenesis have not been entirely clarified, proteolysis of the parent proteins may be one of the important steps in the amyloid fibril formation. Recently, we reported that "dense fibrillar inclusions" (DFI), which had the characteristics of lysosomes and contained organized fibrillar profiles as well, were observed in the reticuloendothelial cells in close association with the foci of new amyloid deposits. We considered the findings as evidence for the involvement of lysosomal system in amyloid fibril formation (l). In the present study, we attempted to determine the identity of the contents of the DFI by the use of antisera against the amyloid protein (AA) and an immuno-electron microscopic technique.Amyloidosis was induced in CBA/J mice by daily injections of casein (l). AA was isolated from amyloid-laden spleens by gel filtration and antibody to it was produced in rabbits (2). For immunocytochemistry, the unlabeled antibody enzyme method (3) was employed.

Sign in / Sign up

Export Citation Format

Share Document