Pentafluorophenyl Ester-Functionalized Phosphorylcholine Polymers: Preparation of Linear, Two-Arm, and Grafted Polymer–Protein Conjugates

2012 ◽  
Vol 13 (7) ◽  
pp. 2099-2109 ◽  
Author(s):  
Samantha McRae ◽  
Xiangji Chen ◽  
Katrina Kratz ◽  
Debasis Samanta ◽  
Elizabeth Henchey ◽  
...  
Keyword(s):  
Grafted Polymer ◽  
Protein Conjugates ◽  
Pentafluorophenyl Ester

Cardiological Biopharmaceuticals in the Conception of Drug Targeting Delivery: Practical Results and Research Perspectives

Acta Naturae ◽  
2012 ◽  
Vol 4 (3) ◽  
pp. 72-81 ◽  
Author(s):  
A. V. Maksimenko
Keyword(s):  
Cell Adhesion Molecules ◽  
Drug Targeting ◽  
Interventional Procedures ◽  
Target Localization ◽  
Clinical Use ◽  
Protein Conjugates ◽  
Research Perspectives ◽  
Significant Interest ◽  
Targeting Delivery ◽  
Molecular Sizes

The results of the clinical use of thrombolytic and antithrombotic preparations developed on the basis of protein conjugates obtained within the framework of the conception of drug targeting delivery in the organism are considered. A decrease has been noted in the number of biomedical projects focused on these derivatives as a result of various factors: the significant depletion of financial and organizational funds, the saturation of the pharmaceutical market with preparations of this kind, and the appearance of original means for interventional procedures. Factors that actively facilitate the conspicuous potentiation of the efficacy of bioconjugates were revealed: the biomedical testing of protein domains and their selected combinations, the optimization of molecular sizes for the bioconjugates obtained, the density of target localization, the application of cell adhesion molecules as targets, and the application of connected enzyme activities. Enzyme antioxidants and the opportunity for further elaboration of the drug delivery conception via the elucidation and formation of therapeutic targets for effective drug reactions by means of pharmacological pre- and postconditioning of myocardium arouse significant interest.

PRODUCTION AND CHARACTERIZATION OF ANTIBODIES ELICITED BY DIFFERENT ALDOSTERONE-PROTEIN CONJUGATES

1972 ◽  
Vol 68 (3_Suppl) ◽  
pp. S32
Author(s):  
W. Vetter ◽  
E. Freedlender ◽  
E. Haber
Keyword(s):  
Protein Conjugates

Exploration of Neem Gum, Acrylamide Grafted Neem Gum and Carboxymethylated Neem Gum as Retardant in Tablet Formulation

2020 ◽  
Vol 16 (9) ◽  
pp. 1404-1410
Author(s):  
Rishabha Malviya
Keyword(s):  
Diclofenac Sodium ◽  
Tablet Formulation ◽  
Angle Of Repose ◽  
Wet Granulation ◽  
Binding Agent ◽  
Grafted Polymer ◽  
Model Drug ◽  
Tablet Dosage ◽  
Tapped Density ◽  
Derivatives Of

Background: In the previous study, investigators have synthesized acrylamide grafted and carboxymethylated derivatives of neem gum and evaluated their potential in the formulation of nanoparticles. In continuation of previous work, authors have evaluated neem gum polysaccharide (NGP), acrylamide grafted neem gum polysaccharide (NGP-g-Am) and carboxymethylated neem gum polysaccharide (CMNGP) as binding agent in the tablet dosage form. Methods: Diclofenac sodium was used as a model drug while microcrystalline cellulose and talc were used as excipient in the preparation of granules employing wet granulation technique. NGP, NGP-g-Am and CMNGP were utilized as binding agent in the preparation of granules. Prepared granules were characterized for various pre-compression and post-compression parameters. Results and Discussion: Binding agents were used in the concentration of 4-24%w/w. NGP incorporated granules showed more bulk density and lower values of tapped density, Carr’s index, bulkiness, Hausner’s ratio and angle of repose as compared to NGP-g-Am consisting granules. NGP-g-Am consisting tablets showed more hardness and zero friability as compared to NGP based tablets. Drug content was found lower for the tablets having grafted polymer in place of NGP. CMNGP were also utilized to prepare granules but granules were not be able to compress keeping all the compacting parameters same as used in the case of NGP and NGP-g-Am consisting granules. NGP and NGP-g-Am were able to sustain drug release up to 6 and 8 h, respectively. Conclusion: It can be concluded that NGP-g-Am induces better properties when used as a binder in the tablet formulation than native polymer, while CMNGP cannot be utilized as a binding agent in the preparation of a tablet.

Preparation of conjugates of uridine with proteins by the imido ester condensation method

1981 ◽  
Vol 46 (4) ◽  
pp. 933-940 ◽  
Author(s):  
Helmut Pischel ◽  
Antonín Holý ◽  
Günther Wagner
Keyword(s):  
Human Serum ◽  
Acid Hydrolysis ◽  
Ester Hydrochloride ◽  
Protein Conjugates ◽  
Condensation Method ◽  
Ester Condensation ◽  
Acid Catalyzed

Reaction of 5'-O-p-toluenesulfonyl-2',3'-O-isopropylideneuridine (I) with sodium 4-cyanophenoxide afforded 2',3'-O-isopropylidene-5'-O-(4-cyanophenyl)uridine (II) which was converted by acid hydrolysis into 5'-O-(4-cyanophenyl)uridine (IIIa). Acid-catalyzed addition of ethanol to compound IIIa gave the imido ester hydrochloride IIIb which on reaction with ammonia or ethylamine was transformed into the amidine derivatives IIIc and IIId. Compound IIIb reacted with human serum albumine or bovine gamma-globuline at pH 9.2 to give protein conjugates with uridine, bound covalently by an amidine bond (IIIe,f).

scholarly journals A western blot approach to detection of human plasma protein conjugates derived from D-penicillamine.

1994 ◽  
Vol 53 (4) ◽  
pp. 256-260 ◽  
Author(s):  
C A Laycock ◽  
M J Phelan ◽  
R C Bucknall ◽  
J W Coleman
Keyword(s):  
Western Blot ◽  
Human Plasma ◽  
Plasma Protein ◽  
Protein Conjugates ◽  
Human Plasma Protein

scholarly journals A New Saccharomyces cerevisiae Strain with a Mutant Smt3-Deconjugating Ulp1 Protein Is Affected in DNA Replication and Requires Srs2 and Homologous Recombination for Its Viability

2004 ◽  
Vol 24 (12) ◽  
pp. 5130-5143 ◽  
Author(s):  
Christine Soustelle ◽  
Laurence Vernis ◽  
Karine Fréon ◽  
Anne Reynaud-Angelin ◽  
Roland Chanet ◽  
...  
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Homologous Recombination ◽  
Nonhomologous End Joining ◽  
End Joining ◽  
Recombination Mechanism ◽  
Growth Defects ◽  
Protein Conjugates ◽  
Synthetically Lethal ◽  
Mutant Cells ◽  
Insight Into

ABSTRACT The Saccharomyces cerevisiae Srs2 protein is involved in DNA repair and recombination. In order to gain better insight into the roles of Srs2, we performed a screen to identify mutations that are synthetically lethal with an srs2 deletion. One of them is a mutated allele of the ULP1 gene that encodes a protease specifically cleaving Smt3-protein conjugates. This allele, ulp1-I615N, is responsible for an accumulation of Smt3-conjugated proteins. The mutant is unable to grow at 37°C. At permissive temperatures, it still shows severe growth defects together with a strong hyperrecombination phenotype and is impaired in meiosis. Genetic interactions between ulp1 and mutations that affect different repair pathways indicated that the RAD51-dependent homologous recombination mechanism, but not excision resynthesis, translesion synthesis, or nonhomologous end-joining processes, is required for the viability of the mutant. Thus, both Srs2, believed to negatively control homologous recombination, and the process of recombination per se are essential for the viability of the ulp1 mutant. Upon replication, mutant cells accumulate single-stranded DNA interruptions. These structures are believed to generate different recombination intermediates. Some of them are fixed by recombination, and others require Srs2 to be reversed and fixed by an alternate pathway.

Graft Polymers Derived from Natural Rubber

1956 ◽  
Vol 29 (1) ◽  
pp. 99-105 ◽  
Author(s):  
G. F. Bloomfield ◽  
F. M. Merrett ◽  
F. J. Popham ◽  
P. Mc L. Swift
Keyword(s):  
Tensile Strength ◽  
Natural Rubber ◽  
Methyl Methacrylate ◽  
Transfer Reaction ◽  
Polymer Chains ◽  
Vinyl Monomers ◽  
Grafted Polymer ◽  
Polymeric Chains ◽  
Graft Polymers ◽  
Direct Growth

Abstract Graft polymers result when vinyl monomers are polymerized in the presence of natural rubber, either in solution or as latex, and some of the polymeric chains become attached to the rubber molecules. The properties of the natural rubber can be widely modified according to the nature and the amount of the grafted polymer. The polymer-modified natural rubber appears to be produced by direct growth of polymer chains on to rubber molecules rather than by a transfer reaction involving the rubber. Graft polymers of styrene and methyl methacrylate with natural rubber can be compounded and cured to give light-colored articles of good tensile strength, and rubber-methyl methacrylate graft polymers have outstanding flex-cracking and fatigue resistance.

Drug protein conjugates—I. A study of the covalent binding of [14C]captopril to plasma proteins in the rat

1982 ◽  
Vol 31 (9) ◽  
pp. 1755-1760 ◽  
Author(s):  
B.Kevin Park ◽  
Peter S. Grabowski ◽  
John H.K. Yeung ◽  
Alasdair M. Breckenridge
Keyword(s):  
Plasma Proteins ◽  
Covalent Binding ◽  
Protein Conjugates
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