Selective Detection of Crystalline Cellulose in Plant Cell Walls with Sum-Frequency-Generation (SFG) Vibration Spectroscopy

2011 ◽  
Vol 12 (7) ◽  
pp. 2434-2439 ◽  
Author(s):  
Anna L. Barnette ◽  
Laura C. Bradley ◽  
Brandon D. Veres ◽  
Edward P. Schreiner ◽  
Yong Bum Park ◽  
...  
Keyword(s):  
Plant Cell ◽  
Cell Walls ◽  
Sum Frequency Generation ◽  
Crystalline Cellulose ◽  
Selective Detection ◽  
Plant Cell Walls ◽  
Sum Frequency ◽  
Vibration Spectroscopy ◽  
Frequency Generation

scholarly journals Inhomogeneity of Cellulose Microfibril Assembly in Plant Cell Walls Revealed with Sum Frequency Generation Microscopy

2018 ◽  
Vol 122 (19) ◽  
pp. 5006-5019 ◽  
Author(s):  
Shixin Huang ◽  
Mohamadamin Makarem ◽  
Sarah N. Kiemle ◽  
Hossein Hamedi ◽  
Moujhuri Sau ◽  
...  
Keyword(s):  
Plant Cell ◽  
Cell Walls ◽  
Cellulose Microfibril ◽  
Sum Frequency Generation ◽  
Plant Cell Walls ◽  
Sum Frequency ◽  
Microfibril Assembly ◽  
Frequency Generation

Plant cell walls to ethanol

2012 ◽  
Vol 442 (2) ◽  
pp. 241-252 ◽  
Author(s):  
Douglas B. Jordan ◽  
Michael J. Bowman ◽  
Jay D. Braker ◽  
Bruce S. Dien ◽  
Ronald E. Hector ◽  
...  
Keyword(s):  
Plant Cell ◽  
Cell Walls ◽  
Second Generation ◽  
Consolidated Bioprocessing ◽  
Enzymatic Saccharification ◽  
Crystalline Cellulose ◽  
Plant Cell Walls ◽  
Second Generation Bioethanol ◽  
Single Organism ◽  
The Cost

Conversion of plant cell walls to ethanol constitutes second generation bioethanol production. The process consists of several steps: biomass selection/genetic modification, physiochemical pretreatment, enzymatic saccharification, fermentation and separation. Ultimately, it is desirable to combine as many of the biochemical steps as possible in a single organism to achieve CBP (consolidated bioprocessing). A commercially ready CBP organism is currently unreported. Production of second generation bioethanol is hindered by economics, particularly in the cost of pretreatment (including waste management and solvent recovery), the cost of saccharification enzymes (particularly exocellulases and endocellulases displaying kcat ~1 s−1 on crystalline cellulose), and the inefficiency of co-fermentation of 5- and 6-carbon monosaccharides (owing in part to redox cofactor imbalances in Saccharomyces cerevisiae).

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