Colloidal Force Spectroscopy and Cell Biological Investigations on Biomimetic Polyelectrolyte Multilayer Coatings Composed of Chondroitin Sulfate and Heparin

2011 ◽  
Vol 12 (6) ◽  
pp. 1987-1997 ◽  
Author(s):  
Steffi Grohmann ◽  
Holger Rothe ◽  
Marion Frant ◽  
Klaus Liefeith
Keyword(s):  
Chondroitin Sulfate ◽  
Force Spectroscopy ◽  
Multilayer Coatings ◽  
Polyelectrolyte Multilayer

Biomimetic multilayer coatings deliver gentamicin and reduce implant-related osteomyelitis in rats

2019 ◽  
Vol 64 (4) ◽  
pp. 383-395
Author(s):  
Steffi Grohmann ◽  
Manuela Menne ◽  
Diana Hesse ◽  
Sabine Bischoff ◽  
René Schiffner ◽  
...  
Keyword(s):  
Orthopedic Surgery ◽  
Material Properties ◽  
Bulk Material ◽  
Local Delivery ◽  
Multilayer Coatings ◽  
Assembly Process ◽  
Polyelectrolyte Multilayer ◽  
Cytotoxic Effects ◽  
Joint Infections ◽  
Main Components

Abstract Implant-related infections like periprosthetic joint infections (PJI) are still a challenging issue in orthopedic surgery. In this study, we present a prophylactic anti-infective approach based on a local delivery of the antibiotic gentamicin. The local delivery is achieved via a nanoscale polyelectrolyte multilayer (PEM) coating that leaves the bulk material properties of the implant unaffected while tuning the surface properties. The main components of the coating, i.e. polypeptides and sulfated glycosaminoglycans (sGAG) render this coating both biomimetic (matrix mimetic) and biodegradable. We show how adaptions in the conditions of the multilayer assembly process and the antibiotic loading process affect the amount of delivered gentamicin. The highest concentration of gentamicin could be loaded into films composed of polypeptide poly-glutamic acid when the pH of the loading solution was acidic. The concentration of gentamicin on the surface could be tailored with the number of deposited PEM layers. The resulting coatings reveal a bacteriotoxic effect on Staphylococcus cells but show no signs of cytotoxic effects on MC3T3-E1 osteoblasts. Moreover, when multilayer-coated titanium rods were implanted into contaminated medullae of rat tibiae, a reduction in the development of implant-related osteomyelitis was observed. This reduction was more pronounced for the multifunctional, matrix-mimetic heparin-based coatings that only deliver lower amounts of gentamicin.

scholarly journals The stability of BMP loaded polyelectrolyte multilayer coatings on titanium

Biomaterials ◽  
2013 ◽  
Vol 34 (23) ◽  
pp. 5737-5746 ◽  
Author(s):  
Raphael Guillot ◽  
Flora Gilde ◽  
Pierre Becquart ◽  
Frédéric Sailhan ◽  
Aurélien Lapeyrere ◽  
...  
Keyword(s):  
Multilayer Coatings ◽  
Polyelectrolyte Multilayer ◽  
The Stability
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