Combined Influence of Substrate Stiffness and Surface Topography on the Antiadhesive Properties of Acr-sP(EO-stat-PO) Hydrogels

2010 ◽  
Vol 11 (12) ◽  
pp. 3375-3383 ◽  
Author(s):  
Vera A. Schulte ◽  
Mar Diez ◽  
Yibing Hu ◽  
Martin Möller ◽  
Marga C. Lensen
Keyword(s):  
Surface Topography ◽  
Substrate Stiffness ◽  
Influence Of Substrate

scholarly journals Atomic force acoustic microscopy reveals the influence of substrate stiffness and topography on cell behavior

2019 ◽  
Vol 10 ◽  
pp. 2329-2337 ◽  
Author(s):  
Yan Liu ◽  
Li Li ◽  
Xing Chen ◽  
Ying Wang ◽  
Meng-Nan Liu ◽  
...  
Keyword(s):  
Acoustic Microscopy ◽  
Substrate Stiffness ◽  
Cell Behavior ◽  
Cell Alignment ◽  
Nondestructive Technique ◽  
Elastic Deformations ◽  
L929 Cells ◽  
Atomic Force ◽  
Cell Substrate ◽  
Influence Of Substrate

The stiffness and the topography of the substrate at the cell–substrate interface are two key properties influencing cell behavior. In this paper, atomic force acoustic microscopy (AFAM) is used to investigate the influence of substrate stiffness and substrate topography on the responses of L929 fibroblasts. This combined nondestructive technique is able to characterize materials at high lateral resolution. To produce substrates of tunable stiffness and topography, we imprint nanostripe patterns on undeveloped and developed SU-8 photoresist films using electron-beam lithography (EBL). Elastic deformations of the substrate surfaces and the cells are revealed by AFAM. Our results show that AFAM is capable of imaging surface elastic deformations. By immunofluorescence experiments, we find that the L929 cells significantly elongate on the patterned stiffness substrate, whereas the elasticity of the pattern has only little effect on the spreading of the L929 cells. The influence of the topography pattern on the cell alignment and morphology is even more pronounced leading to an arrangement of the cells along the nanostripe pattern. Our method is useful for the quantitative characterization of cell–substrate interactions and provides guidance for the tissue regeneration therapy in biomedicine.

Substrate stiffness tunes the dynamics of polyvalent rolling motors

Soft Matter ◽  
2021 ◽  
Author(s):  
Chapin S. Korosec ◽  
Lavisha Jindal ◽  
Mathew Schneider ◽  
Ignacio Calderon de la Barca ◽  
Martin J. Zuckermann ◽  
...  
Keyword(s):  
Substrate Stiffness ◽  
Active Motion ◽  
Influence Of Substrate

Nature utilizes the burnt bridges ratchet (BBR) to generate active motion in a variety of biological contexts. Here, the influence of substrate stiffness on spherical BBR dynamics is investigated.

scholarly journals Influence of the PDMS substrate stiffness on the adhesion of Acanthamoeba castellanii

2014 ◽  
Vol 5 ◽  
pp. 1393-1398 ◽  
Author(s):  
Sören B Gutekunst ◽  
Carsten Grabosch ◽  
Alexander Kovalev ◽  
Stanislav N Gorb ◽  
Christine Selhuber-Unkel
Keyword(s):  
Cell Adhesion ◽  
Elastic Properties ◽  
Mammalian Cells ◽  
Cellular Response ◽  
Acanthamoeba Castellanii ◽  
Substrate Stiffness ◽  
Human Pathogen ◽  
Human Pathogens ◽  
Young’S Moduli ◽  
Influence Of Substrate

Background: Mechanosensing of cells, particularly the cellular response to substrates with different elastic properties, has been discovered in recent years, but almost exclusively in mammalian cells. Much less attention has been paid to mechanosensing in other cell systems, such as in eukaryotic human pathogens. Results: We report here on the influence of substrate stiffness on the adhesion of the human pathogen Acanthamoebae castellanii (A. castellanii). By comparing the cell adhesion area of A. castellanii trophozoites on polydimethylsiloxane (PDMS) substrates with different Young’s moduli (4 kPa, 29 kPa, and 128 kPa), we find significant differences in cell adhesion area as a function of substrate stiffness. In particular, the cell adhesion area of A. castellanii increases with a decreasing Young’s modulus of the substrate. Conclusion: The dependence of A. castellanii adhesion on the elastic properties of the substrate is the first study suggesting a mechanosensory effect for a eukaryotic human pathogen. Interestingly, the main targets of A. castellanii infections in the human body are the eye and the brain, i.e., very soft environments. Thus, our study provides first hints towards the relevance of mechanical aspects for the pathogenicity of eukaryotic parasites.

The influence of substrate stiffness gradients on primary human dermal fibroblasts

Biomaterials ◽  
2013 ◽  
Vol 34 (21) ◽  
pp. 5070-5077 ◽  
Author(s):  
Isabel Hopp ◽  
Andrew Michelmore ◽  
Louise E. Smith ◽  
David E. Robinson ◽  
Akash Bachhuka ◽  
...  
Keyword(s):  
Dermal Fibroblasts ◽  
Substrate Stiffness ◽  
Human Dermal Fibroblasts ◽  
Influence Of Substrate

scholarly journals Influence of Substrate Stiffness on Cell Spread Area

2012 ◽  
Vol 102 (3) ◽  
pp. 703a-704a
Author(s):  
Srikanth Raghavan ◽  
Aravind R. Rammohan ◽  
Martial Hervy
Keyword(s):  
Substrate Stiffness ◽  
Spread Area ◽  
Influence Of Substrate ◽  
Cell Spread
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