Surface-Active and Stimuli-Responsive Polymer−Si(100) Hybrids from Surface-Initiated Atom Transfer Radical Polymerization for Control of Cell Adhesion

2004 ◽  
Vol 5 (6) ◽  
pp. 2392-2403 ◽  
Author(s):  
F. J. Xu ◽  
S. P. Zhong ◽  
L. Y. L. Yung ◽  
E. T. Kang ◽  
K. G. Neoh
Keyword(s):  
Cell Adhesion ◽  
Atom Transfer Radical Polymerization ◽  
Radical Polymerization ◽  
Atom Transfer ◽  
Stimuli Responsive ◽  
Responsive Polymer ◽  
Surface Active ◽  
Stimuli Responsive Polymer

Stability and Cell Adhesion Properties of Poly(N-isopropylacrylamide) Brushes with Variable Grafting Densities

2011 ◽  
Vol 64 (9) ◽  
pp. 1261 ◽  
Author(s):  
Xiaofeng Sui ◽  
Andrea Di Luca ◽  
Michel Klein Gunnewiek ◽  
E. Stefan Kooij ◽  
Clemens A. van Blitterswijk ◽  
...  
Keyword(s):  
Cell Adhesion ◽  
Atom Transfer Radical Polymerization ◽  
Radical Polymerization ◽  
Lower Critical Solution Temperature ◽  
Polymer Brushes ◽  
Pure Water ◽  
Solution Temperature ◽  
Atom Transfer ◽  
Adhesion Properties ◽  
Critical Solution Temperature

Poly(N-isopropylacrylamide) brushes with three different grafting densities were synthesized via surface-initiated atom-transfer radical polymerization on glass or on silicon substrates. The substrates were modified with monochlorosilane-based or trimethoxysilane-based atom-transfer radical polymerization initiators. Atomic force microscopy images showed detachment of brushes from the monochlorosilane-based system under cell culture conditions. In situ ellipsometry demonstrated the reversible swelling and collapse of the brushes as the temperature was varied across the lower critical solution temperature of poly(N-isopropylacrylamide) in pure water. The polymer brushes were evaluated as supporting substrates for MC-3T3 cell cultures. At 37°C (T>lower critical solution temperature), the seeded cells adhered, spread, and proliferated, whereas at 25°C (T<lower critical solution temperature), the cells detached from the surface. The low-density polymer brush showed the highest cell adhesion, featuring adhering cells with an elongated morphology.

scholarly journals Nanohybrid polymer brushes on silica for bioseparation

2016 ◽  
Vol 4 (19) ◽  
pp. 3247-3256 ◽  
Author(s):  
Lingdong Jiang ◽  
Héctor Bagán ◽  
Tripta Kamra ◽  
Tongchang Zhou ◽  
Lei Ye
Keyword(s):  
Atom Transfer Radical Polymerization ◽  
Click Chemistry ◽  
Radical Polymerization ◽  
Polymer Brushes ◽  
Atom Transfer ◽  
Temperature Responsive ◽  
Responsive Polymer

Surface-initiated atom transfer radical polymerization and click chemistry are used to synthesize temperature-responsive polymer brushes for glycoprotein separation.

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