Site-Specific Binding of the 9.5 Kilodalton DNA-Binding Protein ORF80 Visualized by Atomic Force Microscopy

2005 ◽  
Vol 6 (3) ◽  
pp. 1252-1257 ◽  
Author(s):  
M. Lysetska ◽  
H. Zettl ◽  
I. Oka ◽  
G. Lipps ◽  
G. Krauss ◽  
...  
Keyword(s):  
Atomic Force Microscopy ◽  
Dna Binding ◽  
Specific Binding ◽  
Binding Protein ◽  
Dna Binding Protein ◽  
Site Specific ◽  
Force Microscopy ◽  
Atomic Force

Investigation of site-specific DNA binding with nicking endonuclease Nt.BspD6I at single molecule level by atomic force microscopy

BIOPHYSICS ◽  
2012 ◽  
Vol 57 (3) ◽  
pp. 314-317 ◽  
Author(s):  
A. V. Machulin ◽  
E. I. Deryusheva ◽  
A. K. Yunusova ◽  
L. A. Zheleznaya ◽  
I. N. Serdyuk
Keyword(s):  
Atomic Force Microscopy ◽  
Dna Binding ◽  
Single Molecule ◽  
Site Specific ◽  
Nicking Endonuclease ◽  
Single Molecule Level ◽  
Force Microscopy ◽  
Atomic Force

The Specific Binding between Galactose Group and Ricinus Communis Agglutinin (RCA) Investigated with Microcantilever

2012 ◽  
Vol 531-532 ◽  
pp. 600-604
Author(s):  
Hui Yong Zhang ◽  
Ji Hu ◽  
Hui Min Liu
Keyword(s):  
Atomic Force Microscopy ◽  
Self Assembly ◽  
Control Experiment ◽  
Ricinus Communis ◽  
Specific Binding ◽  
Specific Interaction ◽  
Protein Layer ◽  
Force Microscopy ◽  
Atomic Force ◽  
Ricinus Communis Agglutinin

The specific recognization between galactose group and Ricinus Communis Agglutinin (RCA) was investigated by microcantilever. The gold side of the microcantilever was covalently bound with N-galactose, RCA and asialofetuin (ASF) via mixed self assembly monolayer of 11-mercaptoundecanoic acid and 6-mercaptohexanol, respectively. After adding RCA into the flowing cell, the deflection could be observed on the N-galactose or ASF modified microcantilever. Meanwhile, the deflection could also be observed after ASF bound to the RCA modified microcantilever. In order to prove that the deflection is caused by the specific interaction between the galactose group and RCA, bovine serum albumin (BSA) was introduced into the flowing cell as control experiment and no obvious deflection was observed. The specific interaction was also confirmed by the evidence that the bound protein layer can be mechanically removed with atomic force microscopy nanolithography technology.

Assessment of DNA Binding to Human Rad51 Protein by using Quartz Crystal Microbalance and Atomic Force Microscopy: Effects of ADP and BRC4-28 Peptide Inhibitor

ChemPhysChem ◽  
2014 ◽  
Vol 15 (17) ◽  
pp. 3753-3760 ◽  
Author(s):  
Charles Esnault ◽  
Axelle Renodon-Cornière ◽  
Masayuki Takahashi ◽  
Nathalie Casse ◽  
Nicolas Delorme ◽  
...  
Keyword(s):  
Atomic Force Microscopy ◽  
Dna Binding ◽  
Quartz Crystal Microbalance ◽  
Quartz Crystal ◽  
Peptide Inhibitor ◽  
Rad51 Protein ◽  
Force Microscopy ◽  
Atomic Force ◽  
Human Rad51

scholarly journals Site-specific, covalent incorporation of Tus, a DNA-binding protein, on ionic-complementary self-assembling peptide hydrogels using transpeptidase Sortase A as a conjugation tool

Soft Matter ◽  
2013 ◽  
Vol 9 (29) ◽  
pp. 6752 ◽  
Author(s):  
Susanna Piluso ◽  
Heather C. Cassell ◽  
Jonathan L. Gibbons ◽  
Thomas E. Waller ◽  
Nick J. Plant ◽  
...  
Keyword(s):  
Dna Binding ◽  
Binding Protein ◽  
Dna Binding Protein ◽  
Sortase A ◽  
Site Specific ◽  
Self Assembling ◽  
Peptide Hydrogels
Sign in / Sign up

Export Citation Format

Share Document