Polyester Coating of Cellulose Fiber Surfaces Catalyzed by a Cellulose-Binding Module-Candida AntarcticaLipase B Fusion Protein

2004 ◽  
Vol 5 (1) ◽  
pp. 106-112 ◽  
Author(s):  
Malin T. Gustavsson ◽  
Per Valdemar Persson ◽  
Tommy Iversen ◽  
Karl Hult ◽  
Mats Martinelle
Keyword(s):  
Fusion Protein ◽  
Cellulose Fiber ◽  
Cellulose Binding Module ◽  
Cellulose Binding

Does the cellulose-binding module move on the cellulose surface?

Cellulose ◽  
2009 ◽  
Vol 16 (4) ◽  
pp. 587-597 ◽  
Author(s):  
Yu-San Liu ◽  
Yining Zeng ◽  
Yonghua Luo ◽  
Qi Xu ◽  
Michael E. Himmel ◽  
...  
Keyword(s):  
Cellulose Binding Module ◽  
Cellulose Binding ◽  
Cellulose Surface

scholarly journals Cellulose as an inert matrix for presenting cytokines to target cells: production and properties of a stem cell factor–cellulose-binding domain fusion protein

1999 ◽  
Vol 339 (2) ◽  
pp. 429-434
Author(s):  
J. Greg DOHENY ◽  
Eric J. JERVIS ◽  
M. Marta GUARNA ◽  
R. Keith HUMPHRIES ◽  
R. Antony J. WARREN ◽  
...  
Keyword(s):  
Stem Cell ◽  
Fusion Protein ◽  
Stem Cell Factor ◽  
Direct Analysis ◽  
Binding Domain ◽  
Target Cells ◽  
Cellulose Binding Domain ◽  
Binding Characteristics ◽  
Cellulose Binding ◽  
Cellulose Surface

A chimaera of stem cell factor (SCF) and a cellulose-binding domain from the xylanase Cex (CBDCex) effectively immobilizes SCF on a cellulose surface. The fusion protein retains both the cytokine properties of SCF and the cellulose-binding characteristics of CBDCex. When adsorbed on cellulose, SCF–CBDCex is up to 7-fold more potent than soluble SCF–CBDCex and than native SCF at stimulating the proliferation of factor-dependent cell lines. When cells are incubated with cellulose-bound SCF–CBDCex, activated receptors and SCF–CBDCex co-localize on the cellulose matrix. The strong binding of SCF–CBDCex to the cellulose surface permits the effective and localized stimulation of target cells; this is potentially significant for long-term perfusion culturing of factor-dependent cells. It also permits the direct analysis of the effects of surface-bound cytokines on target cells.

Solution Structure of the CBM10 Cellulose Binding Module fromPseudomonasXylanase A†,‡

Biochemistry ◽  
2000 ◽  
Vol 39 (5) ◽  
pp. 978-984 ◽  
Author(s):  
S. Raghothama ◽  
Peter J. Simpson ◽  
Lóránd Szabó ◽  
Tibor Nagy ◽  
Harry J. Gilbert ◽  
...  
Keyword(s):  
Solution Structure ◽  
Cellulose Binding Module ◽  
Cellulose Binding

scholarly journals Cellulose as an inert matrix for presenting cytokines to target cells: production and properties of a stem cell factor‒cellulose-binding domain fusion protein

1999 ◽  
Vol 339 (2) ◽  
pp. 429 ◽  
Author(s):  
J. Greg DOHENY ◽  
Eric J. JERVIS ◽  
M. Marta GUARNA ◽  
R. Keith HUMPHRIES ◽  
R. Antony J. WARREN ◽  
...  
Keyword(s):  
Stem Cell ◽  
Fusion Protein ◽  
Stem Cell Factor ◽  
Binding Domain ◽  
Target Cells ◽  
Cellulose Binding Domain ◽  
Inert Matrix ◽  
Domain Fusion ◽  
Cellulose Binding

Enhancement of acetyl xylan esterase activity on cellulose acetate through fusion to a family 3 cellulose binding module

2015 ◽  
Vol 79-80 ◽  
pp. 27-33 ◽  
Author(s):  
Galina Mai-Gisondi ◽  
Ossi Turunen ◽  
Ossi Pastinen ◽  
Nikolaos Pahimanolis ◽  
Emma R. Master
Keyword(s):  
Cellulose Acetate ◽  
Esterase Activity ◽  
Acetyl Xylan Esterase ◽  
Cellulose Binding Module ◽  
Cellulose Binding
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