scholarly journals Improvement of Stability and Cell Adhesion Properties of Polyelectrolyte Multilayer Films by Chemical Cross-Linking

2004 ◽  
Vol 5 (2) ◽  
pp. 284-294 ◽  
Author(s):  
Ludovic Richert ◽  
Fouzia Boulmedais ◽  
Philippe Lavalle ◽  
Jérôme Mutterer ◽  
Emmanuelle Ferreux ◽  
...  
Keyword(s):  
Cell Adhesion ◽  
Multilayer Films ◽  
Cross Linking ◽  
Polyelectrolyte Multilayer ◽  
Adhesion Properties ◽  
Chemical Cross Linking

Internal Composition versus the Mechanical Properties of Polyelectrolyte Multilayer Films: The Influence of Chemical Cross-Linking†

Langmuir ◽  
2009 ◽  
Vol 25 (24) ◽  
pp. 13809-13819 ◽  
Author(s):  
Thomas Boudou ◽  
Thomas Crouzier ◽  
Rachel Auzély-Velty ◽  
Karine Glinel ◽  
Catherine Picart
Keyword(s):  
Mechanical Properties ◽  
Multilayer Films ◽  
Cross Linking ◽  
Polyelectrolyte Multilayer ◽  
Chemical Cross Linking

Dynamic stiffness of polyelectrolyte multilayer films based on disulfide bonds for in situ control of cell adhesion

2015 ◽  
Vol 3 (38) ◽  
pp. 7546-7553 ◽  
Author(s):  
Li-mei Wang ◽  
Hao Chang ◽  
He Zhang ◽  
Ke-feng Ren ◽  
Huan Li ◽  
...  
Keyword(s):  
Cell Adhesion ◽  
Disulfide Bonds ◽  
Dynamic Stiffness ◽  
Multilayer Films ◽  
Polyelectrolyte Multilayer ◽  
Disulfide Linkages ◽  
In Situ Control

Dynamic stiffness of (poly-l-lysine/hyaluronan-SH) films was developed for in situ control of cell adhesion by using reversible disulfide linkages.

Endothelial cell adhesion on polyelectrolyte multilayer films functionalised with fibronectin and collagen

2012 ◽  
Vol 66 (5) ◽  
Author(s):  
Nahla Zanina ◽  
Soumaya Haddad ◽  
Ali Othmane ◽  
Thierry Jouenne ◽  
David Vaudry ◽  
...  
Keyword(s):  
Endothelial Cells ◽  
Electrochemical Impedance ◽  
Colorimetric Assay ◽  
High Surface ◽  
Multilayer Films ◽  
Polyelectrolyte Multilayer ◽  
Adhesion Properties ◽  
Force Microscopy ◽  
Ohmic Resistance ◽  
Biomaterial Surfaces

AbstractThe seeding of endothelial cells on biomaterial surfaces has become a major challenge to achieve better haemocompatibility of these surfaces. Multilayers of polyelectrolytes formed by the layerby-layer method are promising in this respect. In this study, the interactions of endothelial cells with multilayered polyelectrolytes films were investigated. The build-ups were prepared by selfassembled alternatively adsorbed polyanions and polycations functionalised with fibronectin and collagen. Anionic poly(sodium 4-styrenesulfonate) and cationic poly(allylamine hydrochloride) polyelectrolytes were chosen as a model system. Elaborated surfaces were characterised by electrochemical impedance spectroscopy and cyclic voltammetry. The modified electrode showed good reversible electrochemical properties and high stability in an electrolyte solution. The film ohmic resistance was highest when the film was coated with fibronectin; the parameters so determined were correlated with atomic force microscopy images. Cell colorimetric assay (WST-1) and immunofluorescence were used to quantify the cell viability and evaluate the adhesion properties. When cultured on a surface where proteins were deposited, cells adhered and proliferated better with fibronectin than with collagen. In addition, a high surface free energy was favourable to adhesion and proliferation (48.8 mJ m−2 for fibronectin and 39.7 mJ m−2 for collagen, respectively). Endothelial cells seeded on functionalised-polyelectrolyte multilayer films showed a good morphology and adhesion necessary for the development of a new endothelium.

pH dependent growth of poly(L-lysine)/poly(L-glutamic) acid multilayer films and their cell adhesion properties

2004 ◽  
Vol 570 (1-2) ◽  
pp. 13-29 ◽  
Author(s):  
Ludovic Richert ◽  
Youri Arntz ◽  
Pierre Schaaf ◽  
Jean-Claude Voegel ◽  
Catherine Picart
Keyword(s):  
Cell Adhesion ◽  
Glutamic Acid ◽  
Multilayer Films ◽  
Adhesion Properties ◽  
Ph Dependent ◽  
Dependent Growth

Cross-linking multilayers of poly-l-lysine and hyaluronic acid: Effect on mesenchymal stem cell behavior

2018 ◽  
Vol 41 (4) ◽  
pp. 223-235 ◽  
Author(s):  
Marcus S Niepel ◽  
Fadi Almouhanna ◽  
Bhavya K Ekambaram ◽  
Matthias Menzel ◽  
Andreas Heilmann ◽  
...  
Keyword(s):  
Stem Cell ◽  
Hyaluronic Acid ◽  
Adipogenic Differentiation ◽  
Focal Adhesions ◽  
Stem Cell Differentiation ◽  
Multilayer System ◽  
Polyelectrolyte Multilayers ◽  
Cross Linking ◽  
Polyelectrolyte Multilayer ◽  
Chemical Cross Linking

Background: Cells possess a specialized machinery through which they can sense physical as well as chemical alterations in their surrounding microenvironment that affect their cellular behavior. Aim: In this study, we aim to establish a polyelectrolyte multilayer system of 24 layers of poly-l-lysine and hyaluronic acid to control stem cell response after chemical cross-linking. Methods and results: The multilayer build-up process is monitored using different methods, which show that the studied polyelectrolyte multilayer system grows exponentially following the islands and islets theory. Successful chemical cross-linking is monitored by an increased zeta potential toward negative magnitude and an extraordinary growth in thickness. Human adipose–derived stem cells are used here and a relationship between cross-linking degree and cell spreading is shown as cells seeded on higher cross-linked polyelectrolyte multilayer show enhanced spreading. Furthermore, cells that fail to establish focal adhesions on native and low cross-linked polyelectrolyte multilayer films do not proliferate to a high extent in comparison to cells seeded on highly cross-linked polyelectrolyte multilayer, which also show an increased metabolic activity. Moreover, this study shows the relation between cross-linking degree and human adipose–derived stem cell lineage commitment. Histological staining reveals that highly cross-linked polyelectrolyte multilayers support osteogenic differentiation, whereas less cross-linked and native polyelectrolyte multilayers support adipogenic differentiation in the absence of any specific inducers. Conclusion: Owing to the precise control of polyelectrolyte multilayer properties such as potential, wettability, and viscoelasticity, the system presented here offers great potential for guided stem cell differentiation in regenerative medicine, especially in combination with materials exhibiting a defined surface topography.

scholarly journals Polyelectrolyte Multilayer Films Modification with Ag and rGO Influences Platelets Activation and Aggregate Formation under In Vitro Blood Flow

Nanomaterials ◽  
2020 ◽  
Vol 10 (5) ◽  
pp. 859 ◽  
Author(s):  
Gabriela Imbir ◽  
Aldona Mzyk ◽  
Klaudia Trembecka-Wójciga ◽  
Ewa Jasek-Gajda ◽  
Hanna Plutecka ◽  
...  
Keyword(s):  
Plasma Proteins ◽  
Multilayer Films ◽  
Chemical Parameters ◽  
Polyelectrolyte Multilayer ◽  
Physico Chemical ◽  
Substrate Properties ◽  
Promising Solution ◽  
Blood Plasma Proteins ◽  
Chemical Cross Linking

Surface functionalization of materials to improve their hemocompatibility is a challenging problem in the field of blood-contacting devices and implants. Polyelectrolyte multilayer films (PEMs), which can mimic functions and structure of an extracellular matrix (ECM), are a promising solution to the urgent need for functional blood-contacting coatings. The properties of PEMs can be easily tuned in order to provide a scaffold with desired physico-chemical parameters. In this study chitosan/chondroitin sulfate (Chi/CS) polyelectrolyte multilayers were deposited on medical polyurethane. Afterwards PEMs were modified by chemical cross-linking and nanoparticles introduction. Coatings with variable properties were tested for their hemocompatibility in the cone-plate tester under dynamic conditions. The obtained results enable the understanding of how substrate properties modulate PEMs interaction with blood plasma proteins and the morphotic elements.

In Situ Cross-Linking of Alternating Polyelectrolyte Multilayer Films

Langmuir ◽  
2004 ◽  
Vol 20 (5) ◽  
pp. 1807-1811 ◽  
Author(s):  
Eric R. Welsh ◽  
Caroline L. Schauer ◽  
John P. Santos ◽  
Ronald R. Price
Keyword(s):  
Multilayer Films ◽  
Cross Linking ◽  
Polyelectrolyte Multilayer
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