Genetic Analysis and Characterization of Poly(aspartic acid) Hydrolase-1 fromSphingomonassp. KT-1

2003 ◽  
Vol 4 (1) ◽  
pp. 80-86 ◽  
Author(s):  
Tomohiro Hiraishi ◽  
Mariko Kajiyama ◽  
Kenji Tabata ◽  
Ichiro Yamato ◽  
Yoshiharu Doi
Keyword(s):  
Genetic Analysis ◽  
Aspartic Acid ◽  
Acid Hydrolase

Purification and Characterization of Poly(aspartic acid) Hydrolase fromSphingomonassp. KT-1

2001 ◽  
Vol 2 (4) ◽  
pp. 1155-1160 ◽  
Author(s):  
Kenji Tabata ◽  
Mariko Kajiyama ◽  
Tomohiro Hiraishi ◽  
Hideki Abe ◽  
Ichiro Yamato ◽  
...  
Keyword(s):  
Aspartic Acid ◽  
Acid Hydrolase ◽  
Purification And Characterization

Biochemical and Molecular Characterization of Poly(aspartic acid) Hydrolase-2 fromSphingomonassp. KT-1

2003 ◽  
Vol 4 (5) ◽  
pp. 1285-1292 ◽  
Author(s):  
Tomohiro Hiraishi ◽  
Mariko Kajiyama ◽  
Kenji Tabata ◽  
Hideki Abe ◽  
Ichiro Yamato ◽  
...  
Keyword(s):  
Molecular Characterization ◽  
Aspartic Acid ◽  
Acid Hydrolase

Post-proline endopeptidase, further characterization of the enzyme from pig kidneys

1984 ◽  
Vol 49 (8) ◽  
pp. 1846-1853 ◽  
Author(s):  
Karel Hauzer ◽  
Tomislav Barth ◽  
Linda Servítová ◽  
Karel Jošt
Keyword(s):  
Amino Acids ◽  
Aspartic Acid ◽  
Ion Exchange Chromatography ◽  
Exchange Chromatography ◽  
Relative Molecular Mass ◽  
Enzyme Molecule ◽  
Thiol Compounds ◽  
Modified Method ◽  
N Terminus

A post-proline endopeptidase (EC 3.4.21.26) was isolated from pig kidneys using a modified method described earlier. The enzyme was further purified by ion exchange chromatography on DEAE-Sephacel. The final product contained about 95% of post-proline endopeptidase. The enzyme molecule consisted of one peptide chain with a relative molecular mass of 65 600 to 70 000, containing a large proportion of acidic and alifatic amino acids (glutamic acid, aspartic acid and leucine) and the N-terminus was formed by aspartic acid or asparagine. In order to prevent losses of enzyme activity, thiol compounds has to be added.

scholarly journals Genetic Characterization of Methicillin Resistant and Sensitive, Vancomycin Intermediate Staphylococcus aureus Strains Isolated from Different Iranian Hospitals

2012 ◽  
Vol 2012 ◽  
pp. 1-6 ◽  
Author(s):  
Seyed Asghar Havaei ◽  
Amir Azimian ◽  
Hosein Fazeli ◽  
Mahmood Naderi ◽  
Kiarash Ghazvini ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Genetic Analysis ◽  
Clinical Samples ◽  
Different Types ◽  
Health Care Policies ◽  
Global Health Care ◽  
Vancomycin Resistant ◽  
Epidemiological Evaluation ◽  
Mrsa Strains

Background. Global concerns have been raised due to upward trend of Vancomycin Intermediate Staphylococcus aureus (VISA) and Vancomycin Resistant Staphylococcus aureus (VRSA) reports which mean casting doubt on the absolute effectiveness of the last line of antibiotic treatment for S. aureus, vancomycin. Hence, epidemiological evaluation can improve global health care policies. Methodology. 171 Isolates of Staphylococcus aureus were collected from different types of clinical samples in selected hospitals in Isfahan, Mashhad, and Tehran, Iran. Then, they were evaluated by agar screening, disk diffusion, and MIC method to determine their resistance to vancomycin and methicillin. The isolated VISA strains were then confirmed with genetic analysis by the evaluation of mecA and vanA genes, SCCmec, agr, and spa type, and also toxin profiles. MLST was also performed. Results and Conclusion. Our data indicated that 67% of isolated S. aureus strains were resistant to methicillin. Furthermore, five isolates (2.9%) had intermediate resistance to vancomycin (VISA). In contrast to usual association of VISA with MRSA strains, we found two isolates of MSSA-VISA. Therefore, our data suggests a probable parallel growing trend of VISA towards MSSA, along with MRSA strains. However, more samples are required to confirm these primarily data. Moreover, genetic analysis of the isolated VISA strains revealed that these strains are endemic Asian clones.

Synthesis and characterization of poly(aspartic acid) derivatives conjugated with various amino acids

2010 ◽  
Vol 18 (5) ◽  
pp. 881-890 ◽  
Author(s):  
Ji-Heung Kim ◽  
Chang Mo Son ◽  
Young Sil Jeon ◽  
Woo-Seok Choe
Keyword(s):  
Amino Acids ◽  
Aspartic Acid ◽  
Synthesis And Characterization

scholarly journals Characterization of Trypsin Inhibitor in Florunner Peanut Seeds (Arachis hypogaea L.)1

1988 ◽  
Vol 15 (2) ◽  
pp. 81-84 ◽  
Author(s):  
E. M. Ahmed ◽  
J. A. Applewhite
Keyword(s):  
Molecular Weight ◽  
Amino Acid ◽  
Aspartic Acid ◽  
Arachis Hypogaea ◽  
Trypsin Inhibitor ◽  
Low Molecular Weight ◽  
Arachis Hypogaea L ◽  
Amino Acid Profiles ◽  
Low Levels

Abstract Florunner peanut seeds contained five trypsin isoinhibitors. Amino acid profiles of the trypsin inhibitors fraction showed high levels of aspartic acid, half-cystine and serine and low levels of histidine and tyrosine. The molecular weight of the inhibitor was 8.3 KDa. The presence of multiforms of this inhibitor, its low molecular weight and the high amount of half-cystine indicate that peanut trypsin inhibitor is of the Bowman-Birk type.

Preparation and Characterization of L-aspartic Acid-intercalated Layered Double Hydroxide

2004 ◽  
Vol 52 (1) ◽  
pp. 40-46 ◽  
Author(s):  
Qi Yuan ◽  
Min Wei ◽  
Zhiqiang Wang ◽  
Ge Wang ◽  
Xue Duan
Keyword(s):  
Aspartic Acid ◽  
Layered Double Hydroxide ◽  
Double Hydroxide
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