Interpenetrating Network Formation in Agarose−κ-Carrageenan Gel Composites

2002 ◽  
Vol 3 (3) ◽  
pp. 466-474 ◽  
Author(s):  
Eleonora Amici ◽  
Allan H. Clark ◽  
Valery Normand ◽  
Nick B. Johnson
Keyword(s):  
Network Formation ◽  
Interpenetrating Network

Interpenetrating network formation in isotactic polypropylene/graphene composites

Polymer ◽  
2013 ◽  
Vol 54 (14) ◽  
pp. 3680-3690 ◽  
Author(s):  
Songmei Zhao ◽  
Fenghua Chen ◽  
Chuanzhuang Zhao ◽  
Yingjuan Huang ◽  
Jin-Yong Dong ◽  
...  
Keyword(s):  
Isotactic Polypropylene ◽  
Network Formation ◽  
Interpenetrating Network

Dispersion and Stabilization of Quantum Rod Through Semi-interpenetrating Network Formation

Polymer Korea ◽  
2016 ◽  
Vol 40 (1) ◽  
pp. 130 ◽  
Author(s):  
Listiana Oktavia ◽  
Seong-Jin Kim ◽  
Jong Hyung Kim ◽  
Haein Park ◽  
Peter Chang-Whan Lee ◽  
...  
Keyword(s):  
Network Formation ◽  
Interpenetrating Network ◽  
Quantum Rod

scholarly journals Tunable Fibrin-Alginate Interpenetrating Network Hydrogels to Guide Cell Behavior

2019 ◽  
Author(s):  
Charlotte E. Vorwald ◽  
Tomas Gonzalez-Fernandez ◽  
Shreeya Joshee ◽  
Pawel Sikorski ◽  
J. Kent Leach
Keyword(s):  
Mechanical Properties ◽  
Network Formation ◽  
Mesh Size ◽  
Crosslinking Density ◽  
Biomechanical Properties ◽  
Capillary Network ◽  
Natural Polymers ◽  
Interpenetrating Network ◽  
Tunable Mechanical Properties ◽  
Fibrinogen Content

ABSTRACTHydrogels are effective platforms for use as artificial extracellular matrices, cell carriers, and to present bioactive cues. Two common natural polymers, fibrin and alginate, are broadly used to form hydrogels and have numerous advantages over synthetic materials. Fibrin is a provisional matrix containing native adhesion motifs for cell engagement, yet the interplay between mechanical properties, degradation, and gelation rate is difficult to decouple. Conversely, alginate is highly tunable yet bioinert and requires modification to present necessary adhesion ligands. To address these challenges, we developed a fibrin-alginate interpenetrating network (IPN) hydrogel to combine the desirable adhesion and stimulatory characteristics of fibrin with the tunable mechanical properties of alginate. We tested its efficacy by examining capillary network formation with entrapped co-cultures of mesenchymal stromal cells (MSCs) and endothelial cells (ECs). We manipulated thrombin concentration and alginate crosslinking density independently to modulate the fibrin structure, mesh size, degradation, and biomechanical properties of these constructs. In IPNs of lower stiffness, we observed a significant increase in total cell area (1.72×105 ± 7.9×104 μm2) and circularity (0.56 ± 0.03) compared to cells encapsulated in stiffer IPNs (3.98×104 ± 1.49×104 μm2 and 0.77 ± 0.09, respectively). Fibrinogen content did not influence capillary network formation. However, higher fibrinogen content led to greater retention of these networks confirmed via increased spreading and presence of F-actin at 7 days. This is an elegant platform to decouple cell adhesion and hydrogel bulk stiffness that will be broadly useful for cell instruction and delivery.

Interpenetrating network formation in agarose–sodium gellan gel composites

2001 ◽  
Vol 46 (4) ◽  
pp. 383-391 ◽  
Author(s):  
E. Amici ◽  
A.H. Clark ◽  
V. Normand ◽  
N.B. Johnson
Keyword(s):  
Network Formation ◽  
Interpenetrating Network

scholarly journals Tunable fibrin-alginate interpenetrating network hydrogels to support cell spreading and network formation

2020 ◽  
Vol 108 ◽  
pp. 142-152 ◽  
Author(s):  
Charlotte E. Vorwald ◽  
Tomas Gonzalez-Fernandez ◽  
Shreeya Joshee ◽  
Pawel Sikorski ◽  
J. Kent Leach
Keyword(s):  
Network Formation ◽  
Cell Spreading ◽  
Interpenetrating Network ◽  
Support Cell

Interpenetrating Network Formation in Gellan−Agarose Gel Composites

2000 ◽  
Vol 1 (4) ◽  
pp. 721-729 ◽  
Author(s):  
E. Amici ◽  
A. H. Clark ◽  
V. Normand ◽  
N. B. Johnson
Keyword(s):  
Network Formation ◽  
Agarose Gel ◽  
Interpenetrating Network
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