Structural and Mutational Analysis of Polyethylene Terephthalate–Hydrolyzing Enzyme, Cut190, Based on Three-Dimensional Docking Structure with Model Compounds of Polyethylene Terephthalate

2018 ◽  
pp. 63-75 ◽  
Author(s):  
Takeshi Kawabata ◽  
Masayuki Oda ◽  
Nobutaka Numoto ◽  
Fusako Kawai
Keyword(s):  
Polyethylene Terephthalate ◽  
Mutational Analysis ◽  
Three Dimensional ◽  
Model Compounds

scholarly journals Silvering of three-dimensional polyethylene terephthalate textile material by means of wet-chemical processes

2014 ◽  
Vol 85 (6) ◽  
pp. 658-670 ◽  
Author(s):  
Toty Onggar ◽  
Mohammad Abu Shayed ◽  
Rolf-Dieter Hund ◽  
Chokri Cherif
Keyword(s):  
Polyethylene Terephthalate ◽  
Three Dimensional ◽  
Chemical Processes ◽  
Textile Material ◽  
Wet Chemical

scholarly journals Isolation and Characterization of Mutations in theEscherichia coli Regulatory Protein XapR

1999 ◽  
Vol 181 (14) ◽  
pp. 4397-4403 ◽  
Author(s):  
Casper Jørgensen ◽  
Gert Dandanell
Keyword(s):  
Amino Acids ◽  
Purine Nucleoside Phosphorylase ◽  
Mutational Analysis ◽  
Regulatory Protein ◽  
Three Dimensional ◽  
Dimensional Structure ◽  
Wild Type ◽  
Isolation And Characterization ◽  
In The Wild

ABSTRACT In this work, the LysR-type protein XapR has been subjected to a mutational analysis. XapR regulates the expression of xanthosine phosphorylase (XapA), a purine nucleoside phosphorylase inEscherichia coli. In the wild type, full expression of XapA requires both a functional XapR protein and the inducer xanthosine. Here we show that deoxyinosine can also function as an inducer in the wild type, although not to the same extent as xanthosine. We have isolated and characterized in detail the mutants that can be induced by other nucleosides as well as xanthosine. Sequencing of the mutants has revealed that two regions in XapR are important for correct interactions between the inducer and XapR. One region is defined by amino acids 104 and 132, and the other region, containing most of the isolated mutations, is found between amino acids 203 and 210. These regions, when modelled into the three-dimensional structure of CysB from Klebsiella aerogenes, are placed close together and are most probably directly involved in binding the inducer xanthosine.

Manufacturing Technique and Property Evaluation of RFPET/TPET Hybrid Nonwoven Fabric

2013 ◽  
Vol 365-366 ◽  
pp. 1165-1168
Author(s):  
Jia Horng Lin ◽  
Ya Lan Hsing ◽  
Wen Hao Hsing ◽  
Jin Mao Chen ◽  
Ching Wen Lou
Keyword(s):  
Tensile Strength ◽  
Polyethylene Terephthalate ◽  
Three Dimensional ◽  
Far Infrared ◽  
Nonwoven Fabric ◽  
Air Permeability ◽  
Infrared Emissivity ◽  
Environment Protection ◽  
Nonwoven Fabrics ◽  
Property Evaluation

Heat energy plays a significant role in resources and industries, which makes the development of energy-saving and thermal retention materials important to environment protection. This study combines three-dimensional hollow Polyethylene Terephthalate (TPET) fibers, recycled far-infrared polyethylene terephthalate (RFPET) fibers, and low melting temperature polyethylene terephthalate (LPET) fibers at various ratios to make the RFPET/TPET hybrid nonwoven fabric. The tensile strength, tearing strength, air permeability, and far infrared emissivity of the fabrics are evaluated. With a blending ratio of 8:0:2, the hybrid nonwoven fabrics have the optimum tensile strength of 145 N, tear strength of 184 N, and air permeability of 205 cm3/cm2/s.

Low-Pressure Microwave Plasma Sterilization of Polyethylene Terephthalate Bottles

2008 ◽  
Vol 71 (10) ◽  
pp. 2119-2123 ◽  
Author(s):  
MICHAEL DEILMANN ◽  
HELMUT HALFMANN ◽  
NIKITA BIBINOV ◽  
JOACHIM WUNDERLICH ◽  
PETER AWAKOWICZ
Keyword(s):  
Polyethylene Terephthalate ◽  
Microwave Plasma ◽  
Treatment Time ◽  
Plasma Reactor ◽  
Challenge Test ◽  
Three Dimensional ◽  
Low Pressure ◽  
Dry Process ◽  
Pet Bottles ◽  
Plasma Sterilization

A low-pressure microwave plasma reactor was developed for sterilization of polyethylene terephthalate (PET) bottles. In contrast to the established method using aseptic filling machines based on toxic sterilants, here a microwave plasma is ignited inside a bottle by using a gas mixture of nitrogen, oxygen, and hydrogen. To that effect, a reactor setup was developed based on a Plasmaline antenna allowing for plasma ignition inside three-dimensional packages. A treatment time below 5 s is provided for a reduction of 105 and 104 CFU of Bacillus atrophaeus and Aspergillus niger, respectively, verified by means of a count reduction test. The sterilization results obtained by means of this challenge test are in accordance with requirements for aseptic packaging machines as defined by the U.S. Food and Drug Administration and the German Engineering Federation. The plasma sterilization process developed here for aseptic filling of beverages is a dry process that avoids residues and the use of maximum allowable concentrations of established sterilants, e.g., hydrogen peroxide.

scholarly journals Motogenic and morphogenic activity of epithelial receptor tyrosine kinases.

1996 ◽  
Vol 133 (5) ◽  
pp. 1095-1107 ◽  
Author(s):  
M Sachs ◽  
K M Weidner ◽  
V Brinkmann ◽  
I Walther ◽  
A Obermeier ◽  
...  
Keyword(s):  
Epithelial Cells ◽  
Cell Motility ◽  
Tyrosine Kinases ◽  
Receptor Tyrosine Kinases ◽  
Mutational Analysis ◽  
Three Dimensional ◽  
Branching Morphogenesis ◽  
Met Receptor ◽  
Kidney Epithelial Cells

Receptor tyrosine kinases play essential roles in morphogenesis and differentiation of epithelia. Here we examined various tyrosine kinase receptors, which are preferentially expressed in epithelia (c-met, c-ros, c-neu, and the keratin growth factor [KGF] receptor), for their capacity to induce cell motility and branching morphogenesis of epithelial cells. We exchanged the ligand-binding domain of these receptors by the ectodomain of trkA and could thus control signaling by the new ligand, NGF. We demonstrate here that the tyrosine kinases of c-met, c-ros, c-neu, the KGF receptor, and trkA, but not the insulin receptor, induced scattering and increased motility of kidney epithelial cells in tissue culture. Mutational analysis suggests that SHC binding is essential for scattering and increased cell motility induced by trkA. The induction of motility in epithelial cells is thus an important feature of various receptor tyrosine kinases, which in vivo play a role in embryogenesis and metastasis. In contrast, only the c-met receptor promoted branching morphogenesis of kidney epithelial cells in three-dimensional matrices, which resemble the formation of tubular epithelia in development. Interestingly, the ability of c-met to induce morphogenesis could be transferred to trkA, when in a novel receptor hybrid COOH-terminal sequences of c-met (including Y14 to Y16) were fused to the trkA kinase domain. These data demonstrate that tubulogenesis of epithelia is a restricted activity of tyrosine kinases, as yet only demonstrated for the c-met receptor. We predict the existence of specific substrates that mediate this morphogenesis signal.

scholarly journals Mutational Analysis of the Hexose Transporter ofPlasmodium falciparumand Development of a Three-dimensional Model

2002 ◽  
Vol 277 (34) ◽  
pp. 30942-30949 ◽  
Author(s):  
Suzanne K. Manning ◽  
Charles Woodrow ◽  
Felipe A. Zuniga ◽  
Pavel Iserovich ◽  
Jorge Fischbarg ◽  
...  
Keyword(s):  
Mutational Analysis ◽  
Three Dimensional ◽  
Hexose Transporter ◽  
Dimensional Model ◽  
Three Dimensional Model

scholarly journals TtgV Represses Two Different Promoters by Recognizing Different Sequences

2008 ◽  
Vol 191 (6) ◽  
pp. 1901-1909 ◽  
Author(s):  
Sandy Fillet ◽  
Marisela Vélez ◽  
Duo Lu ◽  
Xiaodong Zhang ◽  
María-Trinidad Gallegos ◽  
...  
Keyword(s):  
Mutational Analysis ◽  
Efflux Pump ◽  
Three Dimensional ◽  
Multidrug Efflux ◽  
Multidrug Efflux Pump ◽  
Mobility Shift ◽  
Alanine Substitution ◽  
Three Dimensional Models ◽  
Electrophoretic Mobility Shift Assays

ABSTRACT Expression of the multidrug efflux pump ttgDEF and ttgGHI operons is modulated in vivo mainly by the TtgV repressor. TtgV is a multidrug recognition repressor that exhibits a DNA binding domain with a long interaction helix comprising residues 47 to 64. The pattern of expression of the two pumps is different in Pseudomonas putida: in the absence of effectors, the promoter for the ttgD gene is silent, whereas the ttgG gene is expressed at a high basal level. This correlates with the fact that TtgV exhibits a higher affinity for the ttgD operator (K D = 10 ± 1 nM) than for the ttgG (K D = 19 ± 1 nM) operator. Sequence analysis revealed that both operators are 40% identical, and mutational analysis of the ttgD and ttgG operators combined with electrophoretic mobility shift assays and in vivo expression analysis suggests that TtgV recognizes an inverted repeat with a high degree of palindromicity around the central axis. We generated a collection of alanine substitution mutants with substitutions between residues 47 and 64 of TtgV. The results of extensive combinations of promoter variants with these TtgV alanine substitution mutants revealed that TtgV modulates expression from ttgD and ttgG promoters through the recognition of both common and different sequences in the two promoters. In this regard, we found that TtgV mutants at residues 48, 50, 53, 54, 60, and 61 failed to bind ttgG but recognized the ttgD operator. TtgV residues R47, R52, L57, and T49 are critical for binding to both operators. Based on three-dimensional models, we propose that these residues contact nucleotides within the major groove of DNA.

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