Effect of Calcium and EDTA on the Uptake of Cadmium and Lead byBrassica junceain Hydroponic Growth Medium

2011 ◽  
pp. 35-50 ◽  
Author(s):  
D. L. Van Engelen ◽  
J. S. Boyd ◽  
K. Ekbia
Keyword(s):  
Growth Medium ◽  
Cadmium And Lead ◽  
Hydroponic Growth

Reclaimed sewage water: a hydroponic growth medium for plants

1978 ◽  
Vol 3 (2) ◽  
pp. 191-199 ◽  
Author(s):  
A. Wallace ◽  
P.M. Patel ◽  
W.L. Berry ◽  
O.R. Lunt
Keyword(s):  
Growth Medium ◽  
Sewage Water ◽  
Hydroponic Growth

Ultrastructural Alterations in Hela Cells Induced by Spider Venom

1967 ◽  
Vol 25 ◽  
pp. 20-21
Author(s):  
Dale E. McClendon ◽  
Paul N. Morgan ◽  
Bernard L. Soloff
Keyword(s):  
Growth Medium ◽  
Mammalian Cells ◽  
Carcinoma Cells ◽  
Venom Protein ◽  
Sterile Saline ◽  
Carbon Coated ◽  
Available Information ◽  
Loxosceles Reclusa ◽  
Essential Growth ◽  
Solution Cultures

It has been observed that minute amounts of venom from the brown recluse spider, Loxosceles reclusa, are capable of producing cytotoxic changes in cultures of certain mammalian cells (Morgan and Felton, 1965). Since there is little available information concerning the effect of venoms on susceptible cells, we have attempted to characterize, at the electron microscope level, the cytotoxic changes produced by the venom of this spider.Cultures of human epithelial carcinoma cells, strain HeLa, were initiated on sterile, carbon coated coverslips contained in Leighton tubes. Each culture was seeded with approximately 1x105 cells contained in 1.5 ml of a modified Eagle's minimum essential growth medium prepared in Hank's balanced salt solution. Cultures were incubated at 36° C. for three days prior to the addition of venom. The venom was collected from female brown recluse spiders and diluted in sterile saline. Protein determinations on the venom-were made according to the spectrophotometric method of Waddell (1956). Approximately 10 μg venom protein per ml of fresh medium was added to each culture after discarding the old growth medium. Control cultures were treated similarly, except that no venom was added. All cultures were reincubated at 36° C.

Effect of Iron Limitation on the Ultrastructure of Agmenellum Quadruplicatum

1981 ◽  
Vol 39 ◽  
pp. 410-411
Author(s):  
L. P. Hardie ◽  
D. L. Balkwill ◽  
S. E. Stevens
Keyword(s):  
Growth Medium ◽  
Green Alga ◽  
Natural Habitat ◽  
Iron Limitation ◽  
Natural Environments ◽  
Blue Green Alga ◽  
Marine Cyanobacterium ◽  
Natural Systems ◽  
Thin Sectioning ◽  
Agmenellum Quadruplicatum

Agmenellum quadruplicatum is a unicellular, non-nitrogen-fixing, marine cyanobacterium (blue-green alga). The ultrastructure of this organism, when grown in the laboratory with all necessary nutrients, has been characterized thoroughly. In contrast, little is known of its ultrastructure in the specific nutrient-limiting conditions typical of its natural habitat. Iron is one of the nutrients likely to limit this organism in such natural environments. It is also of great importance metabolically, being required for both photosynthesis and assimilation of nitrate. The purpose of this study was to assess the effects (if any) of iron limitation on the ultrastructure of A. quadruplicatum. It was part of a broader endeavor to elucidate the ultrastructure of cyanobacteria in natural systemsActively growing cells were placed in a growth medium containing 1% of its usual iron. The cultures were then sampled periodically for 10 days and prepared for thin sectioning TEM to assess the effects of iron limitation.

Smart Plant Growth Control and Monitoring System for Indoor Hydroponic Farming

2019 ◽  
Vol 1 (2) ◽  
Author(s):  
Fina Supegina
Keyword(s):  
Water Level ◽  
Monitoring System ◽  
Growth Control ◽  
Set Point ◽  
Water Solvent ◽  
Growth Room ◽  
Smart Control ◽  
Level Sensor ◽  
Planting Method ◽  
Hydroponic Growth

Hydroponics is one of planting method that use water as a medium of plants growth, in this technique, mineral solution added into the water solvent, allowing the nutrient uptake process by the plants.  Farming by hydroponic method must pay attention to the following parameters namely, temperature, humidity, the level of water needs and nutrients and also the level of sunlight need for photosynthesis process.  This research used hydroponic technique in hydroponic growth room, and  there is a LED growth light as an alternate of sunlight, due to this room is closed without sunlight.  There are outputs displayed in monitoring system namely, temperature sensor, humidity sensor, ultrasound sensor to detect height of the plant and water level sensor to measured height of the water as a medium of the plant.  Results of measured sensor in hydroponic growth room explained as the following:  fan cooler worked when temperature , and humidity  .  Water pump worked when water level is less than 50% accordance set point.  Control on LED Growth Light and LED Bulb when LDR sensor reached set point > 500 in bright condition, and < 500 in dark condition respectively. The average of Time update/received data in thing speak web is 2.4 second. Keywords: Smart Control, Hydroponic, IoT, Monitoring

Cultivation and Harvesting of Selenium-Enriched Ganoderma lingzhi and Spent Medium Using Kudzu Vine as Substrate

2019 ◽  
Vol 18 (2) ◽  
pp. 152-157
Author(s):  
Zeng Xianlu ◽  
Han Fei ◽  
Zhong Yanmei
Keyword(s):  
Growth Medium ◽  
Wheat Bran ◽  
Fruiting Body ◽  
Sodium Selenite ◽  
Human Consumption ◽  
Growth Substrate ◽  
Organic Selenium ◽  
Significant Difference ◽  
Ganoderma Lingzhi ◽  
Run Speed

In order to harvest selenium-enriched fruiting body and spores of Ganoderma lingzhi and spent medium, G. lingzhi was cultivated in kudzu vine as substrate and the bio-transformation of selenite was evaluated. The growth medium consisted of Kudzu vine supplemented with 20% wheat bran or sawdust or none. The growth medium was supplemented with 0, 10, 20, 30, and 50 mg/kg of sodium selenite. We found a significant difference in spawn run speed, fruiting body and spore yields when Kudzu vine was supplemented with wheat bran or sawdust. However, when whole-kudzu vine was used alone as substrate, it resulted in a significantly lower spawn run speed, fruiting body, and spore yields compared with kudzu vine + sawdust substrate and kudzu vine + wheat bran substrate. The selenium content in fruiting body and spores increased with increasing sodium selenite supplementation and approximately equaled half of the selenium in the substrate. No selenite was detected in both the fruiting body and spores. However, in the spent medium when sodium selenite was supplemented at 10, 20, 30, 50 mg/kg, the residual selenite concentration decreased to 0.45, 0.72, 1.29, and 1.95 mg/kg, respectively, suggesting a higher selenite transformation (92.27–93.57%). In conclusion, if Ganoderma fruiting body and spores were to be harvested for human consumption, approximately 50 mg/kg selenite should be added to the growth substrate. On the other hand, if the spent medium was to be used as an organic selenium source, the optimal sodium selenite supplementation level would be 10 mg/kg.

Sign in / Sign up

Export Citation Format

Share Document