Examining Flanking Sequence Specificity and Topological Specificity in the Binding of Various Molecular Types to DNAs Using Restriction Endonuclease Activity Assays

2011 ◽  
pp. 167-180 ◽  
Author(s):  
S. A. Winkle ◽  
E. Duran ◽  
J. Pulido ◽  
G. Santil ◽  
M. Talavera ◽  
...  
Keyword(s):  
Restriction Endonuclease ◽  
Sequence Specificity ◽  
Endonuclease Activity ◽  
Flanking Sequence ◽  
Topological Specificity

An Electrochemical Assay for Restriction Endonuclease Activity Using Graphene Monolayer

2014 ◽  
Vol 161 (12) ◽  
pp. B261-B264 ◽  
Author(s):  
Kamrul Islam ◽  
Rohit Chand ◽  
Dawoon Han ◽  
Ik-Soo Shin ◽  
Yong-Sang Kim
Keyword(s):  
Restriction Endonuclease ◽  
Graphene Monolayer ◽  
Endonuclease Activity ◽  
Electrochemical Assay

Determination of restriction endonuclease activity in toluene lysates of bacterial cells

1984 ◽  
Vol 97 (2) ◽  
pp. 183-185 ◽  
Author(s):  
N. N. Sokolov ◽  
A. B. Fitsner ◽  
�. B. Khoroshutina ◽  
M. Ya. Kheislere
Keyword(s):  
Restriction Endonuclease ◽  
Bacterial Cells ◽  
Endonuclease Activity

scholarly journals A highly conserved endonuclease activity present in Escherichia coli, bovine, and human cells recognizes oxidative DNA damage at sites of pyrimidines.

1987 ◽  
Vol 7 (1) ◽  
pp. 26-32 ◽  
Author(s):  
P W Doetsch ◽  
W D Henner ◽  
R P Cunningham ◽  
J H Toney ◽  
D E Helland
Keyword(s):  
Escherichia Coli ◽  
Dna Damage ◽  
Oxidative Dna Damage ◽  
Uv Light ◽  
Human Fibroblasts ◽  
Human Cells ◽  
Sequence Specificity ◽  
Endonuclease Activity ◽  
E Coli ◽  
Endonuclease Iii

We have compared the sites of nucleotide incision on DNA damaged by oxidizing agents when cleavage is mediated by either Escherichia coli endonuclease III or an endonuclease present in bovine and human cells. E. coli endonuclease III, the bovine endonuclease isolated from calf thymus, and the human endonuclease partially purified from HeLa and CEM-C1 lymphoblastoid cells incised DNA damaged with osmium tetroxide, ionizing radiation, or high doses of UV light at sites of pyrimidines. For each damaging agent studied, regardless of whether the E. coli, bovine, or human endonuclease was used, the same sequence specificity of cleavage was observed. We detected this endonuclease activity in a variety of human fibroblasts derived from normal individuals as well as individuals with the DNA repair deficiency diseases ataxia telangiectasia and xeroderma pigmentosum. The highly conserved nature of such a DNA damage-specific endonuclease suggests that a common pathway exists in bacteria, humans, and other mammals for the reversal of certain types of oxidative DNA damage.

scholarly journals Flanking sequence specificity determines coding microsatellite heteroduplex and mutation rates with defective DNA mismatch repair (MMR)

Oncogene ◽  
2010 ◽  
Vol 29 (15) ◽  
pp. 2172-2180 ◽  
Author(s):  
H Chung ◽  
C G Lopez ◽  
D J Young ◽  
J F Lai ◽  
J Holmstrom ◽  
...  
Keyword(s):  
Mismatch Repair ◽  
Dna Mismatch Repair ◽  
Mutation Rates ◽  
Sequence Specificity ◽  
Flanking Sequence ◽  
Dna Mismatch

Photo-Cross-Linked Oligonucleotide Duplex as a Decoy-DNA for Inhibition of Restriction Endonuclease Activity

2001 ◽  
Vol 29 (4) ◽  
pp. 223-233 ◽  
Author(s):  
Akira Murakami ◽  
Yoko Yamamoto ◽  
Masataka Namba ◽  
Reiko Iwase ◽  
Tetsuji Yamaoka
Keyword(s):  
Restriction Endonuclease ◽  
Endonuclease Activity ◽  
Oligonucleotide Duplex

scholarly journals Restriction endonuclease activity induced by NC-1A virus infection of aChlorella-like green alga

1986 ◽  
Vol 14 (15) ◽  
pp. 6017-6030 ◽  
Author(s):  
Yunnan Xia ◽  
Dwight E. Burbank ◽  
James L. Van Etten
Keyword(s):  
Virus Infection ◽  
Restriction Endonuclease ◽  
Green Alga ◽  
Endonuclease Activity
Sign in / Sign up

Export Citation Format

Share Document