Generation of Lipid Peroxyl Radicals from Oxidized Edible Oils and Heme-Iron: Suppression of DNA Damage by Unrefined Oils and Vegetable Extracts

2002 ◽  
pp. 282-300
Author(s):  
Ayako Kanazawa ◽  
Tomohiro Sawa ◽  
Takaaki Akaike ◽  
Hiroshi Maeda
Keyword(s):  
Dna Damage ◽  
Edible Oils ◽  
Heme Iron ◽  
Peroxyl Radicals ◽  
Lipid Peroxyl Radicals

Generation of lipid peroxyl radicals from edible oils and their biological activities: A need for consideration for anti-radical components and purification processing

BioFactors ◽  
2000 ◽  
Vol 13 (1-4) ◽  
pp. 187-193 ◽  
Author(s):  
Ayako Kanazawa ◽  
Tomohiro Sawa ◽  
Takaaki Akaike ◽  
Shigeru Morimura ◽  
Kenji Kida ◽  
...  
Keyword(s):  
Edible Oils ◽  
Biological Activities ◽  
Peroxyl Radicals ◽  
Lipid Peroxyl Radicals

scholarly journals The mechanism of Fe2+-initiated lipid peroxidation in liposomes: the dual function of ferrous ions, the roles of the pre-existing lipid peroxides and the lipid peroxyl radical

2000 ◽  
Vol 352 (1) ◽  
pp. 27-36 ◽  
Author(s):  
Lixia TANG ◽  
Yong ZHANG ◽  
Zhongming QIAN ◽  
Xun SHEN
Keyword(s):  
Lipid Peroxidation ◽  
Latent Period ◽  
Time Lag ◽  
Lipid Peroxides ◽  
Peroxyl Radicals ◽  
Dual Function ◽  
Critical Ratio ◽  
Ferrous Ions ◽  
Liposomal System ◽  
Lipid Peroxyl Radicals

The mechanism of Fe2+-initiated lipid peroxidation in a liposomal system was studied. It was found that a second addition of ferrous ions within the latent period lengthened the time lag before lipid peroxidation started. The apparent time lag depended on the total dose of Fe2+ whenever the second dose of Fe2+ was added, which indicates that Fe2+ has a dual function: to initiate lipid peroxidation on one hand and suppress the species responsible for the initiation of the peroxidation on the other. When the pre-existing lipid peroxides (LOOH) were removed by incorporating triphenylphosphine into liposomes, Fe2+ could no longer initiate lipid peroxidation and the acceleration of Fe2+ oxidation by the liposomes disappeared. However, when extra LOOH were introduced into liposomes, both enhancement of the lipid peroxidation and shortening of the latent period were observed. When the scavenger of lipid peroxyl radicals (LOOP), N,N´-diphenyl-p-phenylene-diamine, was incorporated into liposomes, neither initiation of the lipid peroxidation nor acceleration of the Fe2+ oxidation could be detected. The results may suggest that both the pre-existing LOOH and LOOP are necessary for the initiation of lipid peroxidation. The latter comes initially from the decomposition of the pre-existing LOOH by Fe2+ and can be scavenged by its reaction with Fe2+. Only when Fe2+ is oxidized to such a degree that LOOP is no longer effectively suppressed does lipid peroxidation start. It seems that by taking the reactions of Fe2+ with LOOH and LOOP into account, the basic chemistry in lipid peroxidation can explain fairly well the controversial phenomena observed in Fe2+-initiated lipid peroxidation, such as the existence of a latent period, the critical ratio of Fe2+ to lipid and the required oxidation of Fe2+.

Plant polyphenols: free radical scavengers or chain-breaking antioxidants?

1995 ◽  
Vol 61 ◽  
pp. 103-116 ◽  
Author(s):  
Catherine Rice-Evans
Keyword(s):  
Biological Effects ◽  
Relative Effectiveness ◽  
Antioxidant Properties ◽  
Peroxyl Radicals ◽  
Free Radical Scavengers ◽  
Dietary Polyphenols ◽  
Chain Breaking ◽  
Lipid Peroxyl Radicals

There is increasing interest in the biological effects of tea- and wine-derived polyphenols and many studies in vitro and in vivo are demonstrating their antioxidant properties. Tea is a major source of dietary polyphenols and an even richer source of the flavanols, the catechins and catechin/gallate esters. Although there are limited studies on the bioavailability of the polyphenols, the absorption of flavanols in humans has been shown. The studies described in this chapter discuss the relative antioxidant potentials of the polyphenolic flavonoids in vitro against radicals generated in the aqueous phase in comparison with their relative effectiveness as antioxidants against propagating lipid peroxyl radicals, and how their activity influences that of α-tocopherol in low-density lipoproteins exposed to oxidative stress.

scholarly journals Drugs Repurposed as Antiferroptosis Agents Suppress Organ Damage, Including AKI, by Functioning as Lipid Peroxyl Radical Scavengers

2019 ◽  
Vol 31 (2) ◽  
pp. 280-296 ◽  
Author(s):  
Eikan Mishima ◽  
Emiko Sato ◽  
Junya Ito ◽  
Ken-ichi Yamada ◽  
Chitose Suzuki ◽  
...  
Keyword(s):  
Lipid Peroxidation ◽  
Cell Death ◽  
Cytochrome P450 ◽  
Liver Injury ◽  
Peroxyl Radical ◽  
Organ Damage ◽  
Peroxyl Radicals ◽  
Radical Scavengers ◽  
Lipid Peroxyl Radicals ◽  
Elevated Lipid

BackgroundFerroptosis, nonapoptotic cell death mediated by free radical reactions and driven by the oxidative degradation of lipids, is a therapeutic target because of its role in organ damage, including AKI. Ferroptosis-causing radicals that are targeted by ferroptosis suppressors have not been unequivocally identified. Because certain cytochrome P450 substrate drugs can prevent lipid peroxidation via obscure mechanisms, we evaluated their antiferroptotic potential and used them to identify ferroptosis-causing radicals.MethodsUsing a cell-based assay, we screened cytochrome P450 substrate compounds to identify drugs with antiferroptotic activity and investigated the underlying mechanism. To evaluate radical-scavenging activity, we used electron paramagnetic resonance–spin trapping methods and a fluorescence probe for lipid radicals, NBD-Pen, that we had developed. We then assessed the therapeutic potency of these drugs in mouse models of cisplatin-induced AKI and LPS/galactosamine-induced liver injury.ResultsWe identified various US Food and Drug Administration–approved drugs and hormones that have antiferroptotic properties, including rifampicin, promethazine, omeprazole, indole-3-carbinol, carvedilol, propranolol, estradiol, and thyroid hormones. The antiferroptotic drug effects were closely associated with the scavenging of lipid peroxyl radicals but not significantly related to interactions with other radicals. The elevated lipid peroxyl radical levels were associated with ferroptosis onset, and known ferroptosis suppressors, such as ferrostatin-1, also functioned as lipid peroxyl radical scavengers. The drugs exerted antiferroptotic activities in various cell types, including tubules, podocytes, and renal fibroblasts. Moreover, in mice, the drugs ameliorated AKI and liver injury, with suppression of tissue lipid peroxidation and decreased cell death.ConclusionsAlthough elevated lipid peroxyl radical levels can trigger ferroptosis onset, some drugs that scavenge lipid peroxyl radicals can help control ferroptosis-related disorders, including AKI.

scholarly journals Nitric Oxide Reaction with Lipid Peroxyl Radicals Spares α-Tocopherol during Lipid Peroxidation

2000 ◽  
Vol 275 (15) ◽  
pp. 10812-10818 ◽  
Author(s):  
Homero Rubbo ◽  
Rafael Radi ◽  
Daniel Anselmi ◽  
Marion Kirk ◽  
Stephen Barnes ◽  
...  
Keyword(s):  
Nitric Oxide ◽  
Lipid Peroxidation ◽  
Peroxyl Radicals ◽  
Lipid Peroxyl Radicals

An electron spin resonance study of the reactions of lipid peroxyl radicals with antioxidants

1990 ◽  
Vol 94 (18) ◽  
pp. 7185-7190 ◽  
Author(s):  
J. Zhu ◽  
W. J. Johnson ◽  
C. L. Sevilla ◽  
J. W. Herrington ◽  
Michael D. Sevilla
Keyword(s):  
Electron Spin Resonance ◽  
Electron Spin ◽  
Peroxyl Radicals ◽  
Electron Spin Resonance Study ◽  
Spin Resonance ◽  
Lipid Peroxyl Radicals ◽  
Spin Resonance Study

scholarly journals Scavenging of lipid peroxyl radicals protects plasma lipids and proteins from peroxynitrite

2018 ◽  
Author(s):  
Ayman Mustafa ◽  
Mahmoud Alfaqih ◽  
Othman Al‑Shboul ◽  
Ahmed Al‑Dwairi
Keyword(s):  
Plasma Lipids ◽  
Peroxyl Radicals ◽  
Lipid Peroxyl Radicals

scholarly journals Guanine-specific DNA damage induced by γ-irradiated histone

2005 ◽  
Vol 388 (3) ◽  
pp. 813-818 ◽  
Author(s):  
Ayako FURUKAWA ◽  
Yusuke HIRAKU ◽  
Shinji OIKAWA ◽  
Catherine LUXFORD ◽  
Michael J. DAVIES ◽  
...  
Keyword(s):  
Dna Damage ◽  
Human Cancer ◽  
Histone H1 ◽  
Peroxyl Radicals ◽  
Sequence Specificity ◽  
Histone H2a ◽  
Γ Irradiation ◽  
Histone Protein ◽  
The Difference ◽  
High Yields

In γ-irradiation, •OH is directly generated from water and causes DNA damage leading to carcinogenesis. Exposure of proteins to γ-irradiation, in the presence of oxygen, gives high yields of hydroperoxides. To clarify whether these hydroperoxides, particularly those formed on DNA-binding histone proteins, participate in γ-irradiation-induced carcinogenesis, experiments using 32P-labelled DNA fragments obtained from human cancer-related genes were undertaken. Histone protein-hydroperoxides induced significant DNA damage in the presence of Cu(I). Histone H1- and H3-hydroperoxides showed stronger DNA damage compared with histone H2A- and H4-hydroperoxides at 0.7 μM. Histone H1-hydroperoxides caused Cu(I)-dependent DNA damage predominantly at guanine residues, especially at 5′-GGC-3′, 5′-GGA-3′, 5′-GGT-3′ and single G bases. In contrast, histone H3-hydroperoxides/Cu(I) induced DNA damage at 5′-G in GG sequences; this sequence specificity is identical with that generated by 2,2′-azobis (2-amidinopropane) dihydrochloride, which is known to produce peroxyl radicals (RO2•). The difference in site specificity of DNA damage induced by histone H1- and H3-hydroperoxides may arise from their amino acid composition or their mode of binding to DNA. The histone H1-hydroperoxides/Cu(I) system also induced 8-oxo-7,8-dihydro-2′-deoxyguanosine formation in calf thymus DNA. It is concluded that histone protein-hydroperoxides can induce guanine-specific DNA damage, which may contribute to γ-irradiation-induced carcinogenesis.

Molecular Imaging of Lipid Peroxyl Radicals in Living Cells with a BODIPY−α-Tocopherol Adduct

Biochemistry ◽  
2009 ◽  
Vol 48 (24) ◽  
pp. 5658-5668 ◽  
Author(s):  
Armen Khatchadourian ◽  
Katerina Krumova ◽  
Sebastien Boridy ◽  
An Thien Ngo ◽  
Dusica Maysinger ◽  
...  
Keyword(s):  
Molecular Imaging ◽  
Living Cells ◽  
Peroxyl Radicals ◽  
Lipid Peroxyl Radicals
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