Plant Uptake of LAS and DEHP from Sludge Amended Soil

2000 ◽  
pp. 99-111 ◽  
Author(s):  
C. Grøn ◽  
F. Laturnus ◽  
G. K. Mortensen ◽  
H. Egsgaard ◽  
L. Samsøe-Petersen ◽  
...  
Keyword(s):  
Plant Uptake ◽  
Amended Soil

Ferrihydrite dissolution at the root zone of crops under semi-arid condition controls the plant uptake of uranium and arsenic

2020 ◽  
Author(s):  
Arindam Malakar ◽  
Michael Kaiser ◽  
Daniel D. Snow ◽  
Harkamal Walia ◽  
Chittaranjan Ray
Keyword(s):  
Plant Uptake ◽  
Root Zone ◽  
Arid Condition ◽  
Semi Arid

Phytostabilization Ability and Growth Response of Calotropis procera and Lantana camara towards Tannery Amended Soil

2019 ◽  
Vol 35 (2) ◽  
pp. 215-224
Author(s):  
Poonam Rani ◽  
◽  
Adarsh Kumar ◽  
Ramesh Chandra Arya ◽  
◽  
...  
Keyword(s):  
Growth Response ◽  
Lantana Camara ◽  
Calotropis Procera ◽  
Amended Soil

Removal of PCR inhibiting substances in sewage sludge amended soil

1995 ◽  
Vol 31 (5-6) ◽  
pp. 311-315 ◽  
Author(s):  
Timothy M. Straub ◽  
Ian L. Pepper ◽  
Charles P. Gerba
Keyword(s):  
Cell Culture ◽  
Enteric Viruses ◽  
Treatment Method ◽  
Cytopathic Effects ◽  
Chelex 100 ◽  
Beef Extract ◽  
Polymerase Chain ◽  
Seminested Pcr ◽  
Soil Colloids ◽  
Amended Soil

Current methods for the detection of enteric viruses in soil involve elution of viruses from soil colloids using beef extract or other proteins. These eluates are then assayed in cell culture and observed daily for cytopathic effects (CPE). While this method is suitable for detection of enteric viruses by cell culture, these eluates contain humic acids and heavy metals that interfere with polymerase chain reaction (PCR) detection. Using beef extract eluates prepared from sludge amended soil, 10 different methods of eluate purification were evaluated for their ability to remove PCR inhibition and maximize sensitivity. The treatment method providing the greatest sensitivity of poliovirus detection by PCR was the combination of Sephadex G-50 and Chelex-100. Using this method 2 plaque forming units (PFU) could be detected after reverse transcription and 30 cycles of PCR. Thirty (30) cycles of seminested PCR were performed on these samples to verify nucleic acid sequences and increase sensitivity after the first 30 cycles of PCR. Using seminested PCR, sensitivity of detection using the Sephadex G-50 and Chelex-100 treatment method to 0.2 PFU. In addition to providing excellent sensitivity for viruses in sludge amended soils, this treatment method is relatively simple compared to other methods.

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