Production of Microbial Cellulases in Transgenic Crop Plants

2000 ◽  
pp. 55-90
Author(s):  
B. S. Hooker ◽  
Z. Dai ◽  
D. B. Anderson ◽  
R. D. Quesenberry ◽  
M. F. Ruth ◽  
...  
Keyword(s):  
Crop Plants ◽  
Transgenic Crop

Suitability of non-lethal marker and marker-free systems for development of transgenic crop plants: Present status and future prospects

2011 ◽  
Vol 29 (6) ◽  
pp. 703-714 ◽  
Author(s):  
P. Manimaran ◽  
G. Ramkumar ◽  
K. Sakthivel ◽  
R.M. Sundaram ◽  
M.S. Madhav ◽  
...  
Keyword(s):  
Present Status ◽  
Crop Plants ◽  
Transgenic Crop ◽  
Future Prospects ◽  
Marker Free

scholarly journals Improving Nitrogen Use Efficiency Through Overexpression of Alanine Aminotransferase in Rice, Wheat, and Barley

2021 ◽  
Vol 12 ◽  
Author(s):  
Jingwen Tiong ◽  
Niharika Sharma ◽  
Ramya Sampath ◽  
Nenah MacKenzie ◽  
Sayuri Watanabe ◽  
...  
Keyword(s):  
Alanine Aminotransferase ◽  
Tca Cycle ◽  
Inducible Promoter ◽  
Crop Plants ◽  
Nitrogen Fertilizers ◽  
Ammonium Uptake ◽  
Transgenic Crop ◽  
Nitrogen Use ◽  
Essential Nutrient ◽  
Research Goal

Nitrogen is an essential nutrient for plants, but crop plants are inefficient in the acquisition and utilization of applied nitrogen. This often results in producers over applying nitrogen fertilizers, which can negatively impact the environment. The development of crop plants with more efficient nitrogen usage is, therefore, an important research goal in achieving greater agricultural sustainability. We utilized genetically modified rice lines over-expressing a barley alanine aminotransferase (HvAlaAT) to help characterize pathways which lead to more efficient use of nitrogen. Under the control of a stress-inducible promoter OsAnt1, OsAnt1:HvAlaAT lines have increased above-ground biomass with little change to both nitrate and ammonium uptake rates. Based on metabolic profiles, carbon metabolites, particularly those involved in glycolysis and the tricarboxylic acid (TCA) cycle, were significantly altered in roots of OsAnt1:HvAlaAT lines, suggesting higher metabolic turnover. Moreover, transcriptomic data revealed that genes involved in glycolysis and TCA cycle were upregulated. These observations suggest that higher activity of these two processes could result in higher energy production, driving higher nitrogen assimilation, consequently increasing biomass production. Other potential mechanisms contributing to a nitrogen-use efficient phenotype include involvements of phytohormonal responses and an alteration in secondary metabolism. We also conducted basic growth studies to evaluate the effect of the OsAnt1:HvAlaAT transgene in barley and wheat, which the transgenic crop plants increased seed production under controlled environmental conditions. This study provides comprehensive profiling of genetic and metabolic responses to the over-expression of AlaAT and unravels several components and pathways which contribute to its nitrogen-use efficient phenotype.

scholarly journals CLONING AND EXPRESSION OF UNIVERSAL STRESS PROTEIN 2 (USP2) GENE IN ESCHERICHIA COLI

2021 ◽  
Vol 2021 (1) ◽  
Author(s):  
A Akram ◽  
K Arshad ◽  
MN Hafeez
Keyword(s):  
Abiotic Stress ◽  
Stress Tolerance ◽  
Protein Gene ◽  
Abiotic Stress Tolerance ◽  
Stress Protein ◽  
Crop Plants ◽  
Cloning And Expression ◽  
Major Type ◽  
Transgenic Crop ◽  
Universal Stress Protein

Different types of abiotic stresses inhibit the normal growth of plants by changing their physical biochemical, morphological, and molecular traits. It links to the polygenic traits, which is controlled with the help of different genes, due to this polygenetic the manipulation of foreign genetic makeup is very difficult. Drought stress is the very major type of threat to reduce the yield of cash crops in Pakistan and as well as in all over the world. Gene manipulation is the solution to face this problem by producing genetically modified crop plants that have the ability to survive in drought conditions. Universal stress protein gene has been already identified in bacteria which showed its response under stressed conditions, by manipulation of universal stress protein gene. It was found from our study that the bacterial cells transformed with the USP2 gene isolated from cotton induced abiotic stress tolerance under heat, osmotic, and salt stress. It was suggested from our findings that the USP2 gene could be used to produce abiotic stress tolerance transgenic crop plants to enhance crop plant yield and quality.

scholarly journals Advances in the development and use of DREB for improved abiotic stress tolerance in transgenic crop plants

2019 ◽  
Vol 25 (6) ◽  
pp. 1323-1334 ◽  
Author(s):  
Tanmoy Sarkar ◽  
Radhakrishnan Thankappan ◽  
Gyan P. Mishra ◽  
Bhagwat D. Nawade
Keyword(s):  
Abiotic Stress ◽  
Stress Tolerance ◽  
Abiotic Stress Tolerance ◽  
Crop Plants ◽  
Transgenic Crop

Expression of Bacillus thuringiensis insecticidal protein genes in transgenic crop plants

1994 ◽  
Vol 49 (1) ◽  
pp. 85-93 ◽  
Author(s):  
Danny Llewellyn ◽  
Yvonne Cousins ◽  
Anne Mathews ◽  
Lynn Hartweck ◽  
Bruce Lyon
Keyword(s):  
Bacillus Thuringiensis ◽  
Crop Plants ◽  
Insecticidal Protein ◽  
Transgenic Crop
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