Determination of Secondary Structure in Protein Aggregates Using Attenuated Total Reflectance FTIR

1999 ◽  
pp. 132-144 ◽  
Author(s):  
A. L. Fink ◽  
S. Seshadri ◽  
R. Khurana ◽  
K. A. Oberg
Keyword(s):  
Secondary Structure ◽  
Protein Aggregates ◽  
Attenuated Total Reflectance ◽  
Total Reflectance ◽  
Reflectance Ftir

Rapid Determination of Free Fatty Acid Content in Waste Deodorizer Distillates Using Single Bounce-Attenuated Total Reflectance-FTIR Spectroscopy

2012 ◽  
Vol 95 (6) ◽  
pp. 1570-1573 ◽  
Author(s):  
Saba Naz ◽  
Sayed Tufail Hussain Sherazi ◽  
Farah N Talpur ◽  
Sarfaraz A Mahesar ◽  
Huseyin Kara
Keyword(s):  
Ftir Spectroscopy ◽  
Quantitative Assessment ◽  
Rapid Determination ◽  
Fatty Acid Content ◽  
Attenuated Total Reflectance ◽  
Calibration Model ◽  
Total Reflectance ◽  
Oil Processing ◽  
Reflectance Ftir

Abstract A simple, rapid, economical, and environmentally friendly analytical method was developed for the quantitative assessment of free fatty acids (FFAs) present in deodorizer distillates and crude oils by single bounce-attenuated total reflectance-FTIR spectroscopy. Partial least squares was applied for the calibration model based on the peak region of the carbonyl group (C=O) from 1726 to 1664 cm–1 associated with the FFAs. The proposed method totally avoided the use of organic solvents or costly standards and could be applied easily in the oil processing industry. The accuracy of the method was checked by comparison to a conventional standard American Oil Chemists' Society (AOCS) titrimetric procedure, which provided good correlation (R = 0.99980), with an SD of ±0.05%. Therefore, the proposed method could be used as an alternate to the AOCS titrimetric method for the quantitative determination of FFAs especially in deodorizer distillates.

scholarly journals Fourier transform infrared-attenuated total reflectance (FTIR-ATR) spectroscopy and chemometric techniques for the determination of adulteration in petrodiesel/biodiesel blends

Química Nova ◽  
2014 ◽  
Vol 37 (3) ◽  
Author(s):  
Armando Guerrero Peña ◽  
Francisco Anguebes Franseschi ◽  
Mepivoseth Castelán Estrada ◽  
Victorino Morales Ramos ◽  
Reyes García Zarracino ◽  
...  
Keyword(s):  
Fourier Transform ◽  
Fourier Transform Infrared ◽  
Attenuated Total Reflectance ◽  
Total Reflectance ◽  
Biodiesel Blends ◽  
Chemometric Techniques ◽  
Reflectance Ftir ◽  
Atr Spectroscopy

Discrimination of clinically relevant Candida species by Fourier-transform infrared spectroscopy with attenuated total reflectance (FTIR-ATR)

RSC Advances ◽  
2016 ◽  
Vol 6 (94) ◽  
pp. 92065-92072 ◽  
Author(s):  
Sónia Silva ◽  
Flávia Tobaldini-Valerio ◽  
Sofia Costa-de-Oliveira ◽  
Mariana Henriques ◽  
Joana Azeredo ◽  
...  
Keyword(s):  
Fourier Transform ◽  
Infrared Spectroscopy ◽  
Fourier Transform Infrared Spectroscopy ◽  
Species Identification ◽  
Candida Species ◽  
Fourier Transform Infrared ◽  
Attenuated Total Reflectance ◽  
Total Reflectance ◽  
Reflectance Ftir

Accurate Candida species identification remains a challenge due to their phenotypic and genotypic similarity.

scholarly journals Collective Ion-Pair Single-Drop Microextraction Attenuated Total Reflectance Fourier Transform Infrared Spectroscopic Determination of Perchlorate in Bioenvironmental Samples

2018 ◽  
Vol 101 (4) ◽  
pp. 1145-1155 ◽  
Author(s):  
Swati Chandrawanshi ◽  
Santosh K Verma ◽  
Manas K Deb
Keyword(s):  
Ftir Spectroscopy ◽  
Ion Pair ◽  
Attenuated Total Reflectance ◽  
Sodium Iodide Symporter ◽  
Single Drop ◽  
Total Reflectance ◽  
Calibration Curves ◽  
Single Drop Microextraction ◽  
Perchlorate Concentration

Abstract Perchlorate (ClO4−) is an environmental pollutant that affects human health. Perchlorate acts as a competitive inhibitor of iodine uptake in the thyroid gland (sodium–iodide symporter inhibitor); thus, its determination is important for public health concerns. Water and milk constitute a significant portion of the human diet. Because regular intake leads to an increase in perchlorate concentration in the human body, the estimation of perchlorate is of great concern. In this work, ion-pair single-drop microextraction (SDME) combined with attenuated total reflectance (ATR)-FTIR spectroscopy has been developed for the determination of perchlorate in bioenvironmental (soil, water, dairy milk, breast milk, and urine) samples. Perchlorate was extracted in a single drop of methyl isobutyl ketone as an - with the cationic surfactant cetyltrimethylammonuim bromide under optimized conditions. The strongest IR peak (at 1076 cm−1) was selected for the quantification of perchlorate among three observed vibrational peaks. Eight calibration curves for different concentration ranges of perchlorate were prepared, and excellent linearity was observed for absorbance and peak area in the range of 0.03–100 ng/mL perchlorate, with r values of 0.977 and 0.976, respectively. The RSDs (n = 8) for the perchlorate concentration ranges of 0.03–100, 0.03–0.5, 0.5–10, and 10–100 ng/mL were in the range of 1.9–2.7% for the above calibration curves. The LOD and LOQ in the present work were 0.003 and 0.02 ng/mL, respectively. The extracted microdrop was analyzed directly by ATR-FTIR spectroscopy. The parameters affecting SDME, i.e, effect of pH, stirring rate, reagent concentration, microdrop volume, and extraction time, were optimized, and the role of foreign species was also investigated. F- and t-tests were performed to check the analytical QA of the method. A noteworthy feature of the reported method is the noninterference of any of the associated ions. The results were compared with those of the ion chromatography MS method, and a high degree of acceptability was found. The method was successfully applied for the determination of perchlorate in bioenvironmental samples.

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