Determination of Metabolites of Nerve Agent O-Ethyl-S-2-Diisopropylaminoethyl Methylphosphonothioate (VX)

1999 ◽  
pp. 369-386 ◽  
Author(s):  
H. Tsuchihashi ◽  
M. Katagi ◽  
M. Tatsuno ◽  
M. Nishikawa ◽  
A. Miki
Keyword(s):  
Nerve Agent

scholarly journals Natural Detoxification Capacity to Inactivate Nerve Agents Sarin and VX in the Rat Blood

2015 ◽  
Vol 58 (4) ◽  
pp. 128-130
Author(s):  
Jiří Bajgar ◽  
Jiří Cabal ◽  
Jiří Kassa ◽  
Michal Pavlík
Keyword(s):  
Cholinesterase Activity ◽  
Normal Activity ◽  
Nerve Agent ◽  
The Other ◽  
Blood Sampling ◽  
Female Rats ◽  
Time Intervals ◽  
Rat Blood

Background: The method of continual determination of the rat blood cholinesterase activity was developed to study the changes of the blood cholinesterases following different intervetions. Aims: The aim of this study is registration of cholinesterase activity in the rat blood and its changes to demonstrate detoxification capacity of rats to inactivate sarin or VX in vivo. Methods: The groups of female rats were premedicated (ketamine and xylazine) and cannulated to a. femoralis. Continual blood sampling (0.02 ml/min) and monitoring of the circulating blood cholinesterase activity were performed. Normal activity was monitored 1–2 min and then the nerve agent was administered i.m. (2× LD50). Using different time intervals of the leg compression and relaxation following the agent injection, cholinesterase activity was monitored and according to the inhibition obtained, detoxification capacity was assessed. Results: Administration of sarin to the leg, then 1 and 5 min compression and 20 min later relaxation showed that further inhibition in the blood was not observed. On the other hand, VX was able to inhibit blood cholinesterases after this intervention. Conclusions: The results demonstrated that sarin can be naturally detoxified on the contrary to VX. Described method can be used as model for other studies dealing with changes of cholinesterases in the blood following different factors.

Highly sensitive retrospective determination of organophosphorous nerve agent biomarkers in human urine implemented in vivo in rabbit

2020 ◽  
Vol 94 (9) ◽  
pp. 3033-3044
Author(s):  
Merav Blanca ◽  
Avital Shifrovitch ◽  
Shlomit Dachir ◽  
Shlomi Lazar ◽  
Maor Elgarisi ◽  
...  
Keyword(s):  
Human Urine ◽  
Nerve Agent ◽  
Highly Sensitive
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