Herbal Laxatives: Influence of Anthrones—Anthraquinones on Energy Metabolism and Ion Transport in a Model System

1998 ◽  
pp. 97-116 ◽  
Author(s):  
H. W. Rauwald
Keyword(s):  
Energy Metabolism ◽  
Ion Transport ◽  
Model System

Ion Transport and Energy Metabolism in Brain

1981 ◽  
Vol 3 (1) ◽  
pp. 107-123 ◽  
Author(s):  
Wayne Crowe ◽  
Avraham Mayevsky ◽  
Leena Mela
Keyword(s):  
Energy Metabolism ◽  
Ion Transport

scholarly journals Impact of a Complex Food Microbiota on Energy Metabolism in the Model OrganismCaenorhabditis elegans

2015 ◽  
Vol 2015 ◽  
pp. 1-12 ◽  
Author(s):  
Elena Zanni ◽  
Chiara Laudenzi ◽  
Emily Schifano ◽  
Claudio Palleschi ◽  
Giuditta Perozzi ◽  
...  
Keyword(s):  
Energy Metabolism ◽  
Model System ◽  
Lactobacillus Delbrueckii ◽  
Altered Expression ◽  
C Elegans ◽  
Gut Colonization ◽  
Evolutionarily Conserved ◽  
Before And After ◽  
Leuconostoc Lactis ◽  
The Impact

The nematodeCaenorhabditis elegansis widely used as a model system for research on aging, development, and host-pathogen interactions. Little is currently known about the mechanisms underlying the effects exerted by foodborne microbes. We took advantage ofC. elegansto evaluate the impact of foodborne microbiota on well characterized physiological features of the worms. Foodborne lactic acid bacteria (LAB) consortium was used to feed nematodes and its composition was evaluated by 16S rDNA analysis and strain typing before and after colonization of the nematode gut.Lactobacillus delbrueckii, L. fermentum, andLeuconostoc lactiswere identified as the main species and shown to display different worm gut colonization capacities. LAB supplementation appeared to decrease nematode lifespan compared to the animals fed with the conventionalEscherichia colinutrient source or a probiotic bacterial strain. Reduced brood size was also observed in microbiota-fed nematodes. Moreover, massive accumulation of lipid droplets was revealed by BODIPY staining. Altered expression ofnhr-49, pept-1, and tub-1genes, associated with obesity phenotypes, was demonstrated by RT-qPCR. Since several pathways are evolutionarily conserved inC. elegans, our results highlight the nematode as a valuable model system to investigate the effects of a complex microbial consortium on host energy metabolism.

Energy metabolism and ion transport studied in single neurons

PROTOPLASMA ◽  
1967 ◽  
Vol 63 (1-3) ◽  
pp. 52-55 ◽  
Author(s):  
Ezio Giacobini
Keyword(s):  
Energy Metabolism ◽  
Ion Transport ◽  
Single Neurons

Correlations between energy metabolism, ion transport, and water content in astrocytes

1992 ◽  
Vol 70 (S1) ◽  
pp. S350-S355 ◽  
Author(s):  
James E. Olson ◽  
Julie A. Evers
Keyword(s):  
Energy Metabolism ◽  
Ion Transport ◽  
Water Content ◽  
Neonatal Rat ◽  
Extracellular Potassium ◽  
Osmotic Swelling ◽  
A Cell ◽  
Phosphate Buffered Saline ◽  
The Brain ◽  
Extracellular Environment

Energy metabolism, ion transport, and water content are interrelated in mechanisms of homeostasis of the brain intracellular and extracellular environment. The simplest model of cell homeostasis, the pump–leak hypothesis, incorporates basic relationships between these variables. Although this model accurately calculates steady-state cell volumes, ion concentrations, and metabolic rates, it fails to predict dynamic changes in these properties during elevated extracellular potassium, metabolic inhibition, and osmotic swelling. We have investigated relationships between ions, energy metabolism, and water content in cerebral astrocytes cultured from the neonatal rat. These cells swell more in hypoosmotic phosphate-buffered saline (PBS) containing NaCl than in hypoosmotic PBS with all NaCl replaced equiosmotically by sucrose. Unidirectional Na+ influx also is greater in cells suspended in hypoosmotic, compared with isoosmotic PBS. These data suggest that astrocytes possess a cell volume dependent mechanism of Na+ accumulation. The influx of Na+ during swelling may be coupled to metabolism via Na–K ATPase and may contribute to the sustained swelling of astrocytes observed in hypoosmotic swelling of the brain in situ.Key words: sodium, hypoosmolality, sodium–potassium adenosine triphosphatase, mathematical modeling.

scholarly journals Phosphorylation of Na+,K+-ATPase at Tyr10 of the α1-Subunit is Suppressed by AMPK and Enhanced by Ouabain in Cultured Kidney Cells

2021 ◽  
Author(s):  
Metka Petrič ◽  
Anja Vidović ◽  
Klemen Dolinar ◽  
Katarina Miš ◽  
Alexander V. Chibalin ◽  
...  
Keyword(s):  
Energy Metabolism ◽  
Ion Transport ◽  
Tyrosine Kinases ◽  
Egf Receptor ◽  
Ion Homeostasis ◽  
Lactate Production ◽  
Serine Phosphorylation ◽  
Whole Body ◽  
Dependent Manner ◽  
Α1 Subunit

AbstractNa+,K+-ATPase (NKA) is essential for maintenance of cellular and whole-body water and ion homeostasis. In the kidney, a major site of ion transport, NKA consumes ~ 50% of ATP, indicating a tight coordination of NKA and energy metabolism. AMP-activated protein kinase (AMPK), a cellular energy sensor, regulates NKA by modulating serine phosphorylation of the α1-subunit, but whether it modulates other important regulatory phosphosites, such as Tyr10, is unknown. Using human kidney (HK-2) cells, we determined that the phosphorylation of Tyr10 was stimulated by the epidermal growth factor (EGF), which was opposed by inhibitors of Src kinases (PP2), tyrosine kinases (genistein), and EGF receptor (EGFR, gefitinib). AMPK activators AICAR and A-769662 suppressed the EGF-stimulated phosphorylation of EGFR (Tyr1173) and NKAα1 at Tyr10. The phosphorylation of Src (Tyr416) was unaltered by AICAR and increased by A-769662. Conversely, ouabain (100 nM), a pharmacological NKA inhibitor and a putative adrenocortical hormone, enhanced the EGF-stimulated Tyr10 phosphorylation without altering the phosphorylation of EGFR (Tyr1173) or Src (Tyr416). Ouabain (100–1000 nM) increased the ADP:ATP ratio, while it suppressed the lactate production and the oxygen consumption rate in a dose-dependent manner. Treatment with ouabain or gene silencing of NKAα1 or NKAα3 subunit did not activate AMPK. In summary, AMPK activators and ouabain had antagonistic effects on the phosphorylation of NKAα1 at Tyr10 in cultured HK-2 cells, which implicates a role for Tyr10 in coordinated regulation of NKA-mediated ion transport and energy metabolism. Graphical Abstract

4.7 Ion Transport and Energy Metabolism

2007 ◽  
pp. 429-465 ◽  
Author(s):  
O. Vergun ◽  
K. E. Dineley ◽  
I. J. Reynolds
Keyword(s):  
Energy Metabolism ◽  
Ion Transport

Cell Biology of Reef-Building Corals: Ion Transport, Acid/Base Regulation, and Energy Metabolism

2016 ◽  
pp. 193-218 ◽  
Author(s):  
Martin Tresguerres ◽  
Katie L. Barott ◽  
Megan E. Barron ◽  
Dimitri D. Deheyn ◽  
David I. Kline ◽  
...  
Keyword(s):  
Energy Metabolism ◽  
Ion Transport ◽  
Cell Biology ◽  
Acid Base
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