Laccase-Producing White-Rot Fungus Lacking Lignin Peroxidase and Manganese Peroxidase

1996 ◽  
pp. 130-150 ◽  
Author(s):  
Claudia Eggert ◽  
Ulrike Temp ◽  
Karl-Erik L. Eriksson
Keyword(s):  
Lignin Peroxidase ◽  
Manganese Peroxidase ◽  
White Rot ◽  
White Rot Fungus

Decoloration of Amaranth by the white-rot fungusTrametes versicolor. Part I. Statistical analysis

2007 ◽  
Vol 53 (2) ◽  
pp. 313-326 ◽  
Author(s):  
Mihaela Gavril ◽  
Peter V. Hodson ◽  
Jim McLellan
Keyword(s):  
Regression Analysis ◽  
Lignin Peroxidase ◽  
Manganese Peroxidase ◽  
Trametes Versicolor ◽  
Stepwise Regression ◽  
White Rot ◽  
White Rot Fungus ◽  
Percent Concentration ◽  
The Relationship ◽  
Decoloration Rate

The white-rot fungus Trametes versicolor decolorized the mono-azo-substituted naphthalenic dye Amaranth. The relationship between the amount of enzymes present in the system and the efficiency of the decoloration process was investigated. The two responses used to quantify the process of decoloration (i.e., initial decoloration rate, v0, and the percent concentration of dye decolorized in 1 h, %c) were correlated with the amount of three enzymes considered for the study (lignin peroxidase, manganese peroxidase, and laccase) and analyzed through stepwise regression analysis (forward, backward, and mixed). The results of the correlation analysis and those of the regression analysis indicated that lignin peroxidase is the enzyme having the greatest influence on the two responses.

scholarly journals Ligninolytic activity of Phlebiopsis gigantea strains in cultivation on Norway spruce wood

2019 ◽  
Vol 25 (1) ◽  
pp. 15-24
Author(s):  
Anna Zolciak
Keyword(s):  
Norway Spruce ◽  
Lignin Peroxidase ◽  
Manganese Peroxidase ◽  
Protocatechuic Acid ◽  
Extracellular Enzymes ◽  
White Rot ◽  
Versatile Peroxidase ◽  
White Rot Fungus ◽  
Spruce Wood ◽  
Phlebiopsis Gigantea

Abstract   As a white-rot basidiomycetous and wood-decaying fungus, Phlebiopsis gigantea (Fr.: Fr.) Jülich is able to degrade lignin, cellulose and hemicellulose with a complex set of extracellular enzymes. Enzyme activity of this fungus has not been sufficiently explored. The aim of this study was to assess the activity of laccase and peroxidases as well as the level of micromolecular compounds in P. gigantea strains, grown on pieces of Norway spruce wood (sapwood and heartwood) over 50 days of incubation under laboratory conditions. Enzymatic activity was determined using spectrophotometry. Phlebiopsis gigantea strains showed laccase (Lacc), manganese peroxidase (MnP), lignin peroxidase (LiP) and versatile peroxidase (VP) activity.  Hydroxy– and methoxyphenols were released during this process also. High levels of MnP activity (from 5.5 to 107.847 mU/μg of protein in cultures on sapwood and from 7.585 to 229.055 mU/μg of protein in cultures on heartwood) were observed in P. gigantea strains, as well as high activity of VP with manganese-oxidizing properties (from 3.36 to 61.708 mU/μg of protein on sapwood and from 1.7 to 254.479 mU/μg of protein on heartwood) compared with the other examined extracellular enzymes. The activity of Lacc ranged from 0 to 0.731 mU/μg of protein on sapwood and from 0 to 0.216 of protein on heartwood. LiP activity was also low and ranged from 0.025 to 0.593 mU/μg of protein on sapwood, and from 0.060 to 1.566 mU/μg of protein on heartwood. The activity of VP in terms of guaiacol-oxidizing properties ranged from 0.016 to 1.432 mU/μg of protein in cultures on sapwood, and from 0.042 to 1.238 mU/μg of protein on heartwood. Released hydroxyphenols for P. gigantea strains ranged from 39.204-129.157 μg of protocatechuic acid/ml in cultures on sapwood, and from 23.098-83.630 μg of protocatechuic acid/ml on heartwood. Methoxyphenols produced by P. gigantea strains ranged from 7.5 to 22.987 μg of vanillin acid/ml on sapwood, and from 10.187 to 36.885 μg of vanillin acid/ml on heartwood. Keywords: white-rot fungus, Phlebiopsis gigantea, laccase, manganese peroxidase, lignin peroxidase, versatile peroxidase, hydroxy–, methoxyphenols.  

The Onset of Lignin-Modifying Enzymes, Decrease of AOX and Color Removal by White-Rot Fungi Grown on Bleach Plant Effluents

1991 ◽  
Vol 24 (3-4) ◽  
pp. 189-198 ◽  
Author(s):  
V. P. Lankinen ◽  
M. M. Inkeröinen ◽  
J. Pellinen ◽  
A. I. Hatakka
Keyword(s):  
Lignin Peroxidase ◽  
Active Form ◽  
White Rot Fungi ◽  
Pulp Mill ◽  
Color Removal ◽  
Effluent Treatment ◽  
White Rot ◽  
White Rot Fungus ◽  
Lignin Peroxidases ◽  
Pulp Mill Effluents

Decrease of adsorbable organic chlorine (AOX) is becoming the most important criterion for the efficiency of pulp mill effluent treatment in the 1990s. Two methods, designated MYCOR and MYCOPOR which utilize the white-rot fungus Phanerochaete chrysosporium have earlier been developed for the color removal of pulp mill effluents, but the processes have also a capacity to decrease the amount of chlorinated organic compounds. Lignin peroxidases (ligninases) produced by P. chrvsosporium may dechlorinate chlorinated phenols. In this work possibilities to use selected white-rot fungi in the treatment of E1-stage bleach plant effluent were studied. Phlebia radiata. Phanerochaete chrvsosporium and Merulius (Phlebia) tremellosus were compared in shake flasks for their ability to produce laccase, lignin peroxidase(s) and manganese-dependent peroxidase(s) and to remove color from a medium containing effluent. Softwood bleaching effluents were treated by carrier-immobilized P. radiata in 2 1 bioreactors and a 10 1 BiostatR -fermentor. Dechlorination was followed using Cl ion and AOX determinations. All fungi removed the color of the effluent. In P. radiata cultivations AOX decrease was ca. 4 mg l−1 in one day. Apparent lignin peroxidase activities as determined by veratryl alcohol oxidation method were negligible or zero in a medium with AOX content of ca. 60 mg l−1, prepared using about 20 % (v/v) of softwood effluent. However, the purification of extracellular enzymes implied that large amounts of lignin peroxidases were present in the medium and, after the purification, in active form. Enzyme proteins were separated using anion exchange chromatography, and they were further characterized by electrophoresis (SDS-PAGE) to reveal the kind of enzymes that were present during AOX decrease and color removal. The most characteristic lignin peroxidase isoenzymes in effluent media were LiP2 and LiP3.

scholarly journals Identification of white rot fungus CP9 and its potential application in biopulping

2018 ◽  
Vol 14 (4) ◽  
pp. 721-726
Author(s):  
Nguyen Thi Hong Lien ◽  
Nguyen Van Hieu ◽  
Luong Thi Hong ◽  
Hy Tuan Anh ◽  
Phan Thi Hong Thao
Keyword(s):  
Rice Straw ◽  
Lignin Peroxidase ◽  
Paper Industry ◽  
Morphological Characteristics ◽  
White Rot Fungi ◽  
White Rot ◽  
White Rot Fungus ◽  
Wood Block ◽  
New Production ◽  
Cellulosic Fibers

Wood-rotting fungi represent an important component of forest ecosystems. Among them, white-rot fungi are the most efficient lignin degraders. Biopulping using white-rot fungi in pretreatment of the materials, is one of the solutions to overcome disadvantages of traditional production methods. Today, the isolation and screening of lignin degrading fungi capable for application in biopulping are of keen interest in Vietnam. The use of non–wood, plant fibres in pulp and paper industry, special, agricultural residuces such as rice and wheat straw, sugarcane baggase, cornstalks etc is the new production toward, potential, serving sustainable development. The fungus CP9, which possessed high ligninolytic activity, was identified and studied in pretreatment of rice straw for biopulping. The fruiting bodies of strain CP9 were effuse on trunk. The hymenium was porous and brown white with short tubes, the white mycelia penetrated wood block. The colony was off-white, blossom, irregularly circular. The mycelia were thick and closely bound together. Beside lignin, this fungus could degrade other substrates such as casein, carboxymethyl cellulose and starch. Biological and morphological characteristics of the fungus CP9 suggested its placement in subdivision Basidiomycota. Combined with the results of phylogenetic analysis, which showed 99% similarity of the fungus with species Leiotrametes lactinea, our strain was named as Leiotrametes lactinea CP9. This fungus could grow well on rice straw under solid state fermentation. Pretreatment of rice straw using L. lactinea CP9 was based on the activity of fungal lignin peroxidase and laccase. After 20 days, the residual enzyme activity was of 21.6 and 18.4 nkat/g material for lignin peroxidase and laccase, respectively. Pretreatment significantly improved the quality of straw, as lignin loss of 38% while cellulosic fibers were comparatively well preserved.

Depolymerization of Straw Lignin by Manganese Peroxidase from Nematoloma frowardii is Accompanied by Release of Carbon Dioxide

Holzforschung ◽  
1999 ◽  
Vol 53 (2) ◽  
pp. 161-166 ◽  
Author(s):  
Martin Hofrichter ◽  
Katrin Scheibner ◽  
Friedemann Bublitz ◽  
Ivonne Schneegaß ◽  
Dirk Ziegenhagen ◽  
...  
Keyword(s):  
Carbon Dioxide ◽  
Manganese Peroxidase ◽  
Gel Permeation Chromatography ◽  
Water Soluble ◽  
White Rot ◽  
White Rot Fungus ◽  
Lignin Fraction ◽  
Molecular Masses ◽  
Total Lignin ◽  
Soluble Part

SummaryManganese peroxidase preparations (MnP) from the white-rot fungusNematoloma frowardiiwere able to release14CO2directly from14C-labeled milled wheat straw (MWS; total lignin fraction) and milled straw lignin (MSL; dioxane soluble part of MWS). Apart from the formation of14CO2(4–10 %) the treatment of insoluble MWS and MSL with MnP resulted in the formation of water-soluble14C-lignin fragments (lignin solubilization, 14–25%). Analyses with gel permeation chromatography (GPC) demonstrated the formation of lignin fragments with predominant molecular masses around 1 kDa. The extent of MWS mineralization and solubilization was enhanced in the presence of reduced glutathione (GSH) acting as thiol mediator, whereas MSL mineralization was not stimulated by GSH. The principle of direct extracellular mineralization of lignin catalyzed by the MnP system may make a significant contribution to the formation of carbon dioxide in lignincellulose containing habitats.

Sign in / Sign up

Export Citation Format

Share Document