Coupling Enzyme Immunoassay with Supercritical Fluid Extraction

1996 ◽  
pp. 422-438 ◽  
Author(s):  
Jerry W. King ◽  
Ki-Souk Nam
Keyword(s):  
Enzyme Immunoassay ◽  
Supercritical Fluid Extraction ◽  
Supercritical Fluid ◽  
Fluid Extraction

Supercritical fluid extraction coupled with enzyme immunoassay analysis of soil herbicides

The Analyst ◽  
1995 ◽  
Vol 120 (10) ◽  
pp. 2617 ◽  
Author(s):  
G. Kim Stearman ◽  
Martha J. M. Wells ◽  
Scott M. Adkisson ◽  
Tadd E. Ridgill
Keyword(s):  
Enzyme Immunoassay ◽  
Supercritical Fluid Extraction ◽  
Supercritical Fluid ◽  
Fluid Extraction

Analytical Supercritical Fluid Extraction

1994 ◽  
Author(s):  
Maria Dolores Luque de Castro ◽  
Miguel Valcárcel ◽  
Maria Teresa Tena
Keyword(s):  
Supercritical Fluid Extraction ◽  
Supercritical Fluid ◽  
Fluid Extraction

VOLATILES IN TAMAIOASA ROMANEASCA VIA SUPERCRITICAL FLUID EXTRACTION (SFE) ANALYSIS

2015 ◽  
Vol 14 (2) ◽  
pp. 297-302 ◽  
Author(s):  
Valeriu V. Cotea ◽  
Lucia Cintia Colibaba ◽  
Liliana Rotaru ◽  
Bogdan Nechita ◽  
Marius Niculaua ◽  
...  
Keyword(s):  
Supercritical Fluid Extraction ◽  
Supercritical Fluid ◽  
Fluid Extraction

scholarly journals Bioactive Compounds Obtained from Polish “Marynka” Hop Variety Using Efficient Two-Step Supercritical Fluid Extraction and Comparison of Their Antibacterial, Cytotoxic, and Anti-Proliferative Activities In Vitro

Molecules ◽  
2021 ◽  
Vol 26 (8) ◽  
pp. 2366
Author(s):  
Katarzyna Klimek ◽  
Katarzyna Tyśkiewicz ◽  
Malgorzata Miazga-Karska ◽  
Agnieszka Dębczak ◽  
Edward Rój ◽  
...  
Keyword(s):  
Supercritical Fluid Extraction ◽  
Supercritical Fluid ◽  
Bioactive Compounds ◽  
Crude Extract ◽  
Normal Skin ◽  
Extraction Methods ◽  
Biological Properties ◽  
Added Value ◽  
Fluid Extraction

Given the health-beneficial properties of compounds from hop, there is still a growing trend towards developing successful extraction methods with the highest yield and also receiving the products with high added value. The aim of this study was to develop efficient extraction method for isolation of bioactive compounds from the Polish “Marynka” hop variety. The modified two-step supercritical fluid extraction allowed to obtain two hop samples, namely crude extract (E1), composed of α-acids, β-acids, and terpene derivatives, as well as pure xanthohumol with higher yield than that of other available methods. The post-extraction residues (R1) were re-extracted in order to obtain extract E2 enriched in xanthohumol. Then, both samples were subjected to investigation of their antibacterial (anti-acne, anti-caries), cytotoxic, and anti-proliferative activities in vitro. It was demonstrated that extract (E1) possessed more beneficial biological properties than xanthohumol. It exhibited not only better antibacterial activity against Gram-positive bacteria strains (MIC, MBC) but also possessed a higher synergistic effect with commercial antibiotics when compared to xanthohumol. Moreover, cell culture experiments revealed that crude extract neither inhibited viability nor divisions of normal skin fibroblasts as strongly as xanthohumol. In turn, calculated selectivity indexes showed that the crude extract had from slightly to significantly better selective anti-proliferative activity towards cancer cells in comparison with xanthohumol.

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