Characterization of Carambola and Yellow Passion Fruit Essences Produced by a Thermally Accelerated Short-Time Evaporation Citrus Evaporator

1995 ◽  
pp. 48-58
Author(s):  
Steven Nagy ◽  
Sandy Barros ◽  
Chin Shu Chen
Keyword(s):  
Passion Fruit ◽  
Short Time ◽  
Yellow Passion Fruit

Development and characterization of microsatellite markers from the yellow passion fruit (Passiflora edulis f. flavicarpa)

2005 ◽  
Vol 5 (2) ◽  
pp. 331-333 ◽  
Author(s):  
E. J. OLIVEIRA ◽  
J. G. PÁDUA ◽  
M. I. ZUCCHI ◽  
L. E. A. CAMARGO ◽  
M. H. P. FUNGARO ◽  
...  
Keyword(s):  
Microsatellite Markers ◽  
Passion Fruit ◽  
Passiflora Edulis ◽  
Yellow Passion Fruit

Definition of morpho-agronomic descriptors for the characterization of yellow passion fruit

2012 ◽  
Vol 145 ◽  
pp. 17-22 ◽  
Author(s):  
Jacqueline Araújo Castro ◽  
Cláudia Garcia Neves ◽  
Onildo Nunes de Jesus ◽  
Eder Jorge de Oliveira
Keyword(s):  
Passion Fruit ◽  
Definition Of ◽  
Yellow Passion Fruit

scholarly journals Characterization of pericarp fractions of yellow passion fruit: density, yield of flour, color, pectin content and degree of esterification

2019 ◽  
Vol 39 (suppl 2) ◽  
pp. 683-689
Author(s):  
Simone Vilela TALMA ◽  
Suelen Alvarenga REGIS ◽  
Patrícia Rodrigues FERREIRA ◽  
Caroline MELLINGER-SILVA ◽  
Eder Dutra de RESENDE
Keyword(s):  
Passion Fruit ◽  
Degree Of Esterification ◽  
Pectin Content ◽  
Flour Color ◽  
Yellow Passion Fruit

scholarly journals Molecular Characterization and Genetic Diversity of Yellow Passion Fruit Based on RAPD Markers

2019 ◽  
Vol 11 (3) ◽  
pp. 575
Author(s):  
Angélica Vieira Sousa Campos ◽  
José Ricardo Peixoto ◽  
Fábio Gelape Faleiro ◽  
Michelle Souza Vilela ◽  
Márcio de Carvalho Pires
Keyword(s):  
Genetic Diversity ◽  
Genetic Variability ◽  
Binary Data ◽  
Rapd Markers ◽  
Molecular Genetic ◽  
Passion Fruit ◽  
Passiflora Edulis ◽  
The University ◽  
Yellow Passion Fruit

Molecular genetic variability studies are essential to complement the agronomic characterization of yellow passion fruit genotypes (Passiflora edulis Sims). Therefore, this study aimed at evaluating the genetic diversity of 24 genotypes of yellow passion fruit obtained from a research program developed by the University of Brasilia and Embrapa Cerrados, using RAPD molecular markers. RAPD markers were obtained from eight decamer primers and converted into a matrix of binary data, from which genetic dissimilarities among genotypes were estimated, and clustering analysis was performed. A total of 54 RAPD markers were obtained, with 6.8 bands per primer on average. From this total, 46 (85.2%) RAPD markers were polymorphic. The OPD10 primer presented the highest number of polymorphic bands. The high percentage of polymorphic markers evidenced the existence of genetic variability among genotypes. Nei’s genetic distance between genotypes ranged from 0.043 to 0.451. Clustering resulted in the formation of at least five groups of similarity.

scholarly journals Molecular characterization of yellow passion fruit genotypes with different yield and disease resistance levels

2018 ◽  
pp. 168-176
Author(s):  
Ana Paula Gomes de Castro ◽  
Anne Pinheiro Costa ◽  
José Ricardo Peixoto ◽  
Fábio Gelape Faleiro ◽  
Michelle de Souza Vilela ◽  
...  
Keyword(s):  
Disease Resistance ◽  
Molecular Characterization ◽  
Passion Fruit ◽  
Yellow Passion Fruit

Rapid, Refined, and Robust Method for Expression, Purification, and Characterization of Recombinant Human Amyloid-beta M1-42

2019 ◽  
Author(s):  
Priya Prakash ◽  
Travis Lantz ◽  
Krupal P. Jethava ◽  
Gaurav Chopra
Keyword(s):  
Amyloid Beta ◽  
Western Blots ◽  
Force Microscopy ◽  
E Coli ◽  
Atomic Force ◽  
Set Up ◽  
Short Time

Amyloid plaques found in the brains of Alzheimer’s disease (AD) patients primarily consists of amyloid beta 1-42 (Ab42). Commercially, Ab42 is synthetized using peptide synthesizers. We describe a robust methodology for expression of recombinant human Ab(M1-42) in Rosetta(DE3)pLysS and BL21(DE3)pLysS competent E. coli with refined and rapid analytical purification techniques. The peptide is isolated and purified from the transformed cells using an optimized set-up for reverse-phase HPLC protocol, using commonly available C18 columns, yielding high amounts of peptide (~15-20 mg per 1 L culture) in a short time. The recombinant Ab(M1-42) forms characteristic aggregates similar to synthetic Ab42 aggregates as verified by western blots and atomic force microscopy to warrant future biological use. Our rapid, refined, and robust technique to purify human Ab(M1-42) can be used to synthesize chemical probes for several downstream in vitro and in vivo assays to facilitate AD research.

Sign in / Sign up

Export Citation Format

Share Document