Frequency Response of Membrane Components in Nerve Axon

Author(s):  
SHIRO TAKASHIMA
Author(s):  
G.P.A. Vigers ◽  
R.A. Crowther ◽  
B.M.F. Pearse

Clathrin forms the polyhedral cage of coated vesicles, which mediate the transfer of selected membrane components within eukaryotic cells. Clathrin cages and coated vesicles have been extensively studied by electron microscopy of negatively stained preparations and shadowed specimens. From these studies the gross morphology of the outer part of the polyhedral coat has been established and some features of the packing of clathrin trimers into the coat have also been described. However these previous studies have not revealed any internal details about the position of the terminal domain of the clathrin heavy chain, the location of the 100kd-50kd accessory coat proteins or the interactions of the coat with the enclosed membrane.


Author(s):  
Michael Edidin

Cell surface membranes are based on a fluid lipid bilayer and models of the membranes' organization have emphasised the possibilities for lateral motion of membrane lipids and proteins within the bilayer. Two recent trends in cell and membrane biology make us consider ways in which membrane organization works against its inherent fluidity, localizing both lipids and proteins into discrete domains. There is evidence for such domains, even in cells without obvious morphological polarity and organization [Table 1]. Cells that are morphologically polarised, for example epithelial cells, raise the issue of membrane domains in an accute form.The technique of fluorescence photobleaching and recovery, FPR, was developed to measure lateral diffusion of membrane components. It has also proven to be a powerful tool for the analysis of constraints to lateral mobility. FPR resolves several sorts of membrane domains, all on the micrometer scale, in several different cell types.


1990 ◽  
Vol 137 (5) ◽  
pp. 290 ◽  
Author(s):  
J.L. Douce ◽  
L. Balmer
Keyword(s):  

2012 ◽  
Vol 132 (8) ◽  
pp. 630-637
Author(s):  
Toru Wakimoto ◽  
Yoshimitsu Takahashi ◽  
Norihito Kimura ◽  
Yukitoshi Narumi ◽  
Naoki Hayakawa

2009 ◽  
Vol 129 (4) ◽  
pp. 517-525 ◽  
Author(s):  
Satoru Miyazaki ◽  
Yoshinobu Mizutani ◽  
Hiroshi Suzuki ◽  
Michiharu Ichikawa

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