Characterization of Cobalt(II)-Substituted Peptide Deformylase:  Function of the Metal Ion and the Catalytic Residue Glu-133†

Biochemistry ◽  
2000 ◽  
Vol 39 (4) ◽  
pp. 779-790 ◽  
Author(s):  
P. T. Ravi Rajagopalan ◽  
Stephanie Grimme ◽  
Dehua Pei
Keyword(s):  
Metal Ion ◽  
Peptide Deformylase ◽  
Catalytic Residue

scholarly journals Preparation and characterization of nucleotide-free and metal ion-free p21 “apoprotein”.

1987 ◽  
Vol 262 (18) ◽  
pp. 8455-8458 ◽  
Author(s):  
J Feuerstein ◽  
R S Goody ◽  
A Wittinghofer
Keyword(s):  
Metal Ion

scholarly journals Preparation and characterization of andisol-zeolite-fly ash-activated carbon (AZFC) as potential adsorbent for metal ion removal

2020 ◽  
Author(s):  
Antonius Agus Bambang Haryanto ◽  
Ari Handono Ramelan ◽  
MTh Sri Budiastuti ◽  
Pranoto
Keyword(s):  
Activated Carbon ◽  
Fly Ash ◽  
Metal Ion ◽  
Metal Ion Removal ◽  
Ion Removal

Highly Selective Separation and Enrichment of Phosphopeptides by Uranyl-Salophen Immobilized Silica Gel Material

2015 ◽  
Vol 1095 ◽  
pp. 341-344 ◽  
Author(s):  
Can Hui Xu ◽  
Guang Liang Zhang ◽  
Xin Zhou ◽  
Xi Lin Xiao ◽  
Chang Ming Nie ◽  
...  
Keyword(s):  
Silica Gel ◽  
Metal Ion ◽  
High Selectivity ◽  
Separation Efficiency ◽  
Solid Phase ◽  
Tetradentate Ligand ◽  
Peptide Mixtures ◽  
New Material ◽  
Solid Phase Material

The characterization of phosphoproteins requires highly specific methods for the separation and enrichment of phosphopeptides. Here we report a novel metal ion-immobilized solid phase material for the separation and enrichment of phosphopeptides. The material is uranyl-salophen-silica gel (USSG) particles in which salophen is a tetradentate ligand of uranyl ion. In USSG salophen is connected on the surface of silica gel and uranyl is bound on the surface through its coordination with salophen. Phosphopeptides can be selectively retained by USSG because uranyl-salophen can bind phosphate moiety with strong affinity and high selectivity. The new material USSG has been successfully used for the separation of phosphopeptides from peptide mixtures with the separation efficiency of 97.0% to 97.4%.

scholarly journals Synthesis, Characterization of a New Polyacrylic Acid Superabsorbent, Some Heavy Metal Ion Sorption, the Adsorption Isotherms, and Quantum Chemical Investigation

Materials ◽  
2020 ◽  
Vol 13 (19) ◽  
pp. 4390
Author(s):  
Sevil Savaskan Yilmaz ◽  
Nuri Yildirim ◽  
Murat Misir ◽  
Yasin Misirlioglu ◽  
Emre Celik
Keyword(s):  
Heavy Metal ◽  
Quantum Chemical ◽  
Metal Ion ◽  
Molecular Mechanic ◽  
Ion Concentration ◽  
Heavy Metal Ion ◽  
Competitive Sorption ◽  
Cross Linker ◽  
The Cross

Poly(acrylic acid/Kryptofix 23-Dimethacrylate) superabsorbent polymer [P (AA/Kry23-DM) SAP] was synthesized by solution polymerization to remove Co, Ni, Cu, Cd, Mn, Zn, Pb, Cr, and Fe ions in water and improve the quality of the water. Kry23-DM cross-linker (1,4,7,13,16-Pentaoxa-10,19 diazo cyclohexene icosane di methacrylate) was synthesized using Kry23 and methacryloyl chloride. The characterization of the molecules was done by FTIR, TGA, DSC, and SEM techniques. The effects of parameters such as pH, concentration, and the metal ion interaction on the heavy metal ions uptaking of SAP was investigated. It was observed that P (AA/Kry23-DM) SAP has maximum water absorption, and the absorption increases with the pH increase. Adsorption rates and sorption capacity, desorption ratios, competitive sorption (qcs), and distribution coefficient (log D) of P(AA/Kry23-DM) SAP were studied as a function of time and pH with the heavy metal ion concentration. Langmuir and Freundlich isotherms of the P (AA/Kry23-DM) SAP were investigated to verify the metal uptake. Molecular mechanic (MM2), Assisted Model Building with Energy Refinement (AMBER), and optimized potentials for liquid simulations (OPLS) methods. were used in quantum chemical calculations for the conformational analysis of the cross-linker and the SAP. ΔH0f calculations of the cross-linker and the superabsorbent were made using Austin Model 1(AM1) method.

Characterization of Electrospray Ionization (ESI) Parameters on In-ESI Hydrogen/Deuterium Exchange of Carbohydrate-Metal Ion Adducts

2018 ◽  
Vol 30 (2) ◽  
pp. 235-247 ◽  
Author(s):  
O. Tara Liyanage ◽  
Matthew R. Brantley ◽  
Emvia I. Calixte ◽  
Touradj Solouki ◽  
Kevin L. Shuford ◽  
...  
Keyword(s):  
Electrospray Ionization ◽  
Metal Ion ◽  
Deuterium Exchange ◽  
Hydrogen Deuterium Exchange ◽  
Hydrogen Deuterium

scholarly journals Synthesis and characterization of Ni(II), Cu(II) and Co(III) complexes with polyamine-containing macrocycles bearing an aminoethyl pendant arm

2004 ◽  
Vol 69 (8-9) ◽  
pp. 641-649 ◽  
Author(s):  
Fouzia Rafat ◽  
M.Y. Siddiqi ◽  
K.S. Siddiqi
Keyword(s):  
Metal Ion ◽  
Magnetic Moments ◽  
Carbon Chain ◽  
Macrocyclic Complex ◽  
Synthesis And Characterization ◽  
Epr Spectrum ◽  
Pendant Arm ◽  
Octahedral Environment ◽  
Electrical Conductivities

Reaction of [M(ppn)2]X2 (where M = Cu(II), Ni(II), Co(II) and ppn = 1,3-diaminopropane) with formaldehyde and ethylenediamine in methanol results in the ready formation of a 16-membered macrocyclic complex. The complexes were characterized by elemental analysis, IR, EPR, electronic spectral data magnetic moments and conductance measurements. The Cu(II), Ni(II) and Co(III) complexes are coordinated axially with both pendant groups of the hexadentate macrocycle. These pendant donors are attached to the macrocycle by a carbon chain. The electrical conductivities of the Cu(II) and Ni(II) chelates indicated them to be 1:2 electrolytes whilst those of Co(III) is a 1:3 electrolyte in DMSO. The EPR spectrum of the copper complex exhibited G at 3.66, which indicates a considerable exchange interaction in the complex. Spectroscopic evidence suggests that in all of the complexes the metal ion is in an octahedral environment.

scholarly journals Extraction and Characterization of Cathepsin Inhibitor from Milkfish

2015 ◽  
Vol 18 (1) ◽  
Author(s):  
Tati Nurhayati ◽  
Ruddy Suwandi ◽  
Syefri Rusyadi
Keyword(s):  
Protease Inhibitor ◽  
Metal Ion ◽  
Proteolytic Enzyme ◽  
Cysteine Proteases ◽  
Partial Purification ◽  
Optimum Temperature ◽  
Endogenous Inhibitors ◽  
Protease Enzyme ◽  
Myofibril Protein

Proteolytic enzyme is distributed acros all organism including fish. Cysteine proteases are the largest group of proteolytic enzyme. Lysosomal cathepsin, one of cysteine protease enzyme, cause softening and degradation of myofibril protein and it’s activity is regulated by endogenous inhibitors. The purposes of this study were to optimize the extraction cathepsin inhibitors from the skin, muscles, and viscera of fish, to partially purify the cathepsin inhibitors of selected sources, and to study the characteristics of the cathepsin inhibitor. The cathepsin inhibitor could be extracted from muscle fish and partially purified using ammonium sulfate of 70%. The purified cathepsin inhibitor had optimum temperature at 40°C and the optimum at pH 8. Metal ions decreased the activity of the protease inhibitor, except 1 mM of metal ion Mn2+ and Na+. Keywords: Cathepsin, characterization, partial purification, protease inhibitor

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