Direct Observation of the Self-Association of Dilute Proteins in the Presence of Inert Macromolecules at High Concentration via Tracer Sedimentation Equilibrium:  Theory, Experiment, and Biological Significance

Biochemistry ◽  
1999 ◽  
Vol 38 (29) ◽  
pp. 9379-9388 ◽  
Author(s):  
Germán Rivas ◽  
Javier A. Fernandez ◽  
Allen P. Minton
Keyword(s):  
Direct Observation ◽  
Biological Significance ◽  
Equilibrium Theory ◽  
The Self ◽  
Sedimentation Equilibrium ◽  
High Concentration ◽  
Self Association

scholarly journals Self-buffering capacity of a human sulfatase for central nervous system delivery

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Yi Wen ◽  
Nazila Salamat-Miller ◽  
Keethkumar Jain ◽  
Katherine Taylor
Keyword(s):  
Central Nervous System ◽  
Nervous System ◽  
Buffering Capacity ◽  
The Self ◽  
High Concentration ◽  
Formulation Design ◽  
The Central Nervous System ◽  
Therapeutic Enzymes ◽  
Intrathecal Space

AbstractDirect delivery of therapeutic enzymes to the Central Nervous System requires stringent formulation design. Not only should the formulation design consider the delicate balance of existing ions, proteins, and osmolality in the cerebrospinal fluid, it must also provide long term efficacy and stability for the enzyme. One fundamental approach to this predicament is designing formulations with no buffering species. In this study, we report a high concentration, saline-based formulation for a human sulfatase for its delivery into the intrathecal space. A high concentration formulation (≤ 40 mg/mL) was developed through a series of systematic studies that demonstrated the feasibility of a self-buffered formulation for this molecule. The self-buffering capacity phenomenon was found to be a product of both the protein itself and potentially the residual phosphates associated with the protein. To date, the self-buffered formulation for this molecule has been stable for up to 4 years when stored at 5 ± 3 °C, with no changes either in the pH values or other quality attributes of the molecule. The high concentration self-buffered protein formulation was also observed to be stable when exposed to multiple freeze–thaw cycles and was robust during in-use and agitation studies.

Monte Carlo Study of the Self-association of Methanol in Benzene

1991 ◽  
Vol 6 (4-6) ◽  
pp. 299-310 ◽  
Author(s):  
Y. Adachi ◽  
K. Nakanishi
Keyword(s):  
Monte Carlo ◽  
Monte Carlo Study ◽  
The Self ◽  
Self Association
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