Structural and Spectral Response of Green Fluorescent Protein Variants to Changes in pH†,‡

Biochemistry ◽  
1999 ◽  
Vol 38 (17) ◽  
pp. 5296-5301 ◽  
Author(s):  
Marc-André Elsliger ◽  
Rebekka M. Wachter ◽  
George T. Hanson ◽  
Karen Kallio ◽  
S. James Remington
Keyword(s):  
Green Fluorescent Protein ◽  
Fluorescent Protein ◽  
Spectral Response ◽  
Green Fluorescent ◽  
Protein Variants

Green Fluorescent Protein Variants as Ratiometric Dual Emission pH Sensors. 3. Temperature Dependence of Proton Transfer†

Biochemistry ◽  
2005 ◽  
Vol 44 (24) ◽  
pp. 8701-8711 ◽  
Author(s):  
Tim B. McAnaney ◽  
Xinghua Shi ◽  
Paul Abbyad ◽  
Henry Jung ◽  
S. James Remington ◽  
...  
Keyword(s):  
Green Fluorescent Protein ◽  
Proton Transfer ◽  
Temperature Dependence ◽  
Fluorescent Protein ◽  
Ph Sensors ◽  
Dual Emission ◽  
Green Fluorescent ◽  
Protein Variants

Green Fluorescent Protein variants fold differentially in prokaryotic and eukaryotic cells

2001 ◽  
Vol 81 (S36) ◽  
pp. 117-128 ◽  
Author(s):  
Andrea Sacchetti ◽  
Valeria Cappetti ◽  
Pierfrancesco Marra ◽  
Roberta Dell'Arciprete ◽  
Tarek El Sewedy ◽  
...  
Keyword(s):  
Green Fluorescent Protein ◽  
Fluorescent Protein ◽  
Eukaryotic Cells ◽  
Green Fluorescent ◽  
Protein Variants

scholarly journals New Unstable Variants of Green Fluorescent Protein for Studies of Transient Gene Expression in Bacteria

1998 ◽  
Vol 64 (6) ◽  
pp. 2240-2246 ◽  
Author(s):  
Jens Bo Andersen ◽  
Claus Sternberg ◽  
Lars Kongsbak Poulsen ◽  
Sara Petersen Bjørn ◽  
Michael Givskov ◽  
...  
Keyword(s):  
Gene Expression ◽  
Green Fluorescent Protein ◽  
Fluorescent Protein ◽  
Transient Gene Expression ◽  
In Situ Monitoring ◽  
Reporter Protein ◽  
Temporal Gene Expression ◽  
Green Fluorescent ◽  
Protein Variants

ABSTRACT Use of the green fluorescent protein (Gfp) from the jellyfishAequorea victoria is a powerful method for nondestructive in situ monitoring, since expression of green fluorescence does not require any substrate addition. To expand the use of Gfp as a reporter protein, new variants have been constructed by the addition of short peptide sequences to the C-terminal end of intact Gfp. This rendered the Gfp susceptible to the action of indigenous housekeeping proteases, resulting in protein variants with half-lives ranging from 40 min to a few hours when synthesized in Escherichia coli andPseudomonas putida. The new Gfp variants should be useful for in situ studies of temporal gene expression.

Green Fluorescent Protein Variants as Ratiometric Dual Emission pH Sensors. 1. Structural Characterization and Preliminary Application†

Biochemistry ◽  
2002 ◽  
Vol 41 (52) ◽  
pp. 15477-15488 ◽  
Author(s):  
George T. Hanson ◽  
Tim B. McAnaney ◽  
Eun Sun Park ◽  
Marla E. P. Rendell ◽  
Daniel K. Yarbrough ◽  
...  
Keyword(s):  
Green Fluorescent Protein ◽  
Structural Characterization ◽  
Fluorescent Protein ◽  
Ph Sensors ◽  
Dual Emission ◽  
Green Fluorescent ◽  
Protein Variants ◽  
Preliminary Application
Sign in / Sign up

Export Citation Format

Share Document