Characterization of (+) Strand Initiation and Termination Sequences Located at the Center of the Equine Infectious Anemia Virus Genome†

Biochemistry ◽  
1999 ◽  
Vol 38 (12) ◽  
pp. 3656-3667 ◽  
Author(s):  
Scott R. Stetor ◽  
Jason W. Rausch ◽  
Mao-Jun Guo ◽  
Janet P. Burnham ◽  
Lawrence R. Boone ◽  
...  
Keyword(s):  
Equine Infectious Anemia Virus ◽  
Virus Genome ◽  
Equine Infectious Anemia ◽  
Strand Initiation ◽  
Anemia Virus

scholarly journals Characterization of Equine Infectious Anemia Virus Integration in the Horse Genome

Viruses ◽  
2015 ◽  
Vol 7 (6) ◽  
pp. 3241-3260 ◽  
Author(s):  
Qiang Liu ◽  
Xue-Feng Wang ◽  
Jian Ma ◽  
Xi-Jun He ◽  
Xiao-Jun Wang ◽  
...  
Keyword(s):  
Equine Infectious Anemia Virus ◽  
Horse Genome ◽  
Equine Infectious Anemia ◽  
Anemia Virus ◽  
Virus Integration

scholarly journals Characterization of a Cytolytic Strain of Equine Infectious Anemia Virus

2003 ◽  
Vol 77 (4) ◽  
pp. 2385-2399 ◽  
Author(s):  
Wendy Maury ◽  
Patrick J. Wright ◽  
Sarahann Bradley
Keyword(s):  
In Vitro Selection ◽  
Equine Infectious Anemia Virus ◽  
Fibroblast Cell ◽  
Open Reading Frames ◽  
Fusogenic Activity ◽  
Equine Infectious Anemia ◽  
Anemia Virus ◽  
Receptor Interference

ABSTRACT A novel strain of equine infectious anemia virus (EIAV) called vMA-1c that rapidly and specifically killed infected equine fibroblasts (ED cells) but not other infectible cell lines was established. This strain was generated from an avirulent, noncytopathic strain of EIAV, MA-1. Studies with this new cytolytic strain of virus have permitted us to define viral parameters associated with EIAV-induced cell killing and begin to explore the mechanism. vMA-1c infection resulted in induction of rapid cell death, enhanced fusogenic activity, and increased rates of spread in equine fibroblasts compared to other strains of EIAV. The highly cytolytic nature of vMA-1c suggested that this strain might be superinfecting equine fibroblasts. Receptor interference studies demonstrated that prior infection of equine fibroblasts with EIAV did not alter the ability of vMA-1c to infect and kill these cells. In similar studies in a canine fibroblast cell line, receptor interference did occur. vMA-1c infection of equine fibroblasts was also associated with large quantities of unintegrated viral DNA, a well-established hallmark of retroviral superinfection. Cloning of the vMA-1c genome identified nucleotide changes that would result in at least one amino acid change in all viral proteins. A chimeric infectious molecular clone containing the vMA-1c tat, S2, and env open reading frames recapitulated most of the characteristics of vMA-1c, including superinfection, fibroblast killing, and fusogenic activity. In summary, in vitro selection for a strain of EIAV that rapidly killed cells resulted in the generation of a virus that was able to superinfect these cells, presumably by the use of a novel mechanism of cell entry. This phenotype mapped to the 3′ half of the genome.

Purification and partial characterization of equine infectious anemia virus reverse transcriptase

Virology ◽  
1991 ◽  
Vol 185 (1) ◽  
pp. 387-394 ◽  
Author(s):  
Anthony Devico ◽  
Ronald C. Montelaro ◽  
Robert C. Gallo ◽  
M.G. Sarngadharan
Keyword(s):  
Reverse Transcriptase ◽  
Equine Infectious Anemia Virus ◽  
Partial Characterization ◽  
Equine Infectious Anemia ◽  
Anemia Virus

scholarly journals Further Characterization of Equine Foamy Virus Reveals Unusual Features among the Foamy Viruses

2002 ◽  
Vol 76 (14) ◽  
pp. 7220-7227 ◽  
Author(s):  
Charles-Henri Lecellier ◽  
Manuel Neves ◽  
Marie-Lou Giron ◽  
Joelle Tobaly-Tapiero ◽  
Ali Saïb
Keyword(s):  
Equine Infectious Anemia Virus ◽  
Foamy Virus ◽  
Nucleotide Sequence Analysis ◽  
Donor And Acceptor ◽  
Foamy Viruses ◽  
Electron Microscopy Analysis ◽  
Equine Infectious Anemia ◽  
Microscopy Analysis ◽  
Anemia Virus

ABSTRACT Foamy viruses (FVs) are nonpathogenic, widely spread complex retroviruses which have been isolated in nonhuman primates, cattle, cats, and more recently in horses. The equine foamy virus (EFV) was isolated from healthy horses and was characterized by molecular cloning and nucleotide sequence analysis. Here, to further characterize this new FV isolate, the location of the transcriptional cap and poly(A) addition sites as well as the main splice donor and acceptor sites were determined, demonstrating the existence of the specific subgenomic pol mRNA, one specific feature of FVs. Moreover, similar to what has been described for the human foamy virus (HFV), the prototype of FVs, a replication-defective EFV genome was identified during persistent infection. At the protein level, the use of specific antibodies allowed us to determine the size and the subcellular localization of EFV Gag, Env, and Tas, the viral transactivators. While EFV Gag was detected in both the cytoplasm and the nucleus, EFV Env mainly localized in the Golgi complex, in contrast to HFV Env, which is sequestered in the endoplasmic reticulum. In addition, electron microscopy analysis demonstrated that EFV budding occurs at the plasma membrane and not intracellularly, as is the case for primate FVs. Interestingly, EFV Tas was detected both in the nucleus and the cytoplasm of Tas-transfected cells, in contrast to the strict nuclear localization of other FV Tas but similar to the equine infectious anemia virus Tat gene product. Taken together, our results reveal that this new FV isolate exhibits remarkable features among FVs, bringing new insights into the biology of these unconventional retroviruses.

Molecular detection, histopathological analysis, and immunohistochemical characterization of equine infectious anemia virus in naturally infected equids

2020 ◽  
Vol 165 (6) ◽  
pp. 1333-1342 ◽  
Author(s):  
Bruna Lopes Bueno ◽  
Rebeca Jéssica Falcão Câmara ◽  
Matheus Vilardo Lóes Moreira ◽  
Grazielle Cossenzo Florentino Galinari ◽  
Francielli Martins Souto ◽  
...  
Keyword(s):  
Molecular Detection ◽  
Equine Infectious Anemia Virus ◽  
Histopathological Analysis ◽  
Equine Infectious Anemia ◽  
Anemia Virus

scholarly journals Characterization of equine infectious anemia virus long terminal repeat.

1987 ◽  
Vol 61 (3) ◽  
pp. 743-747 ◽  
Author(s):  
D Derse ◽  
P L Dorn ◽  
L Levy ◽  
R M Stephens ◽  
N R Rice ◽  
...  
Keyword(s):  
Long Terminal Repeat ◽  
Equine Infectious Anemia Virus ◽  
Terminal Repeat ◽  
Equine Infectious Anemia ◽  
Anemia Virus

scholarly journals Purification and characterization of recombinant equine infectious anemia virus reverse transcriptase.

1991 ◽  
Vol 65 (12) ◽  
pp. 7004-7007 ◽  
Author(s):  
S F Le Grice ◽  
M Panin ◽  
R C Kalayjian ◽  
N J Richter ◽  
G Keith ◽  
...  
Keyword(s):  
Reverse Transcriptase ◽  
Equine Infectious Anemia Virus ◽  
Purification And Characterization ◽  
Equine Infectious Anemia ◽  
Anemia Virus
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