Egg White Sulfhydryl Oxidase:  Kinetic Mechanism of the Catalysis of Disulfide Bond Formation†

Biochemistry ◽  
1999 ◽  
Vol 38 (10) ◽  
pp. 3211-3217 ◽  
Author(s):  
Karen L. Hoober ◽  
Colin Thorpe
Keyword(s):  
Disulfide Bond ◽  
Bond Formation ◽  
Kinetic Mechanism ◽  
Egg White ◽  
Disulfide Bond Formation ◽  
Sulfhydryl Oxidase

scholarly journals Disulfide bond formation activity of soybean quiescin sulfhydryl oxidase

FEBS Journal ◽  
2014 ◽  
Vol 281 (23) ◽  
pp. 5341-5355 ◽  
Author(s):  
Aya Okuda ◽  
Motonori Matsusaki ◽  
Yuki Higashino ◽  
Taro Masuda ◽  
Reiko Urade
Keyword(s):  
Disulfide Bond ◽  
Bond Formation ◽  
Disulfide Bond Formation ◽  
Sulfhydryl Oxidase ◽  
Quiescin Sulfhydryl Oxidase

scholarly journals Per Os Infectivity Factor 5 Identified as a Substrate of P33 in the Baculoviral Disulfide Bond Formation Pathway

2020 ◽  
Vol 94 (15) ◽  
Author(s):  
Huanyu Zhang ◽  
Wenhua Kuang ◽  
Cheng Chen ◽  
Yu Shang ◽  
Xiaoyan Ma ◽  
...  
Keyword(s):  
Disulfide Bond ◽  
Disulfide Bonds ◽  
Bond Formation ◽  
Oral Infection ◽  
Progeny Virus ◽  
Disulfide Bond Formation ◽  
Sulfhydryl Oxidase ◽  
Dna Viruses ◽  
Formation Pathway

ABSTRACT Disulfide bonds are critical for the structure and function of many proteins. Some large DNA viruses encode their own sulfhydryl oxidase for disulfide bond formation. Previous studies have demonstrated that the baculovirus-encoded sulfhydryl oxidase P33 is necessary for progeny virus production, and its enzymatic activity is important for morphogenesis and oral infectivity of baculoviruses. However, the downstream substrates of P33 in the putative redox pathway of baculoviruses are unknown. In this study, we showed that PIF5, one of the per os infectivity factors (PIFs), contained intramolecular disulfide bonds and that the disulfide bond formation was interrupted in the absence of P33. In vivo pulldown and colocalization analyses revealed that PIF5 and P33 interacted with each other during virus infection. Further, in vitro assays validated that the reduced PIF5 proteins could be oxidized by P33. To understand the contribution of disulfide bonds to the function of PIF5, several cysteine-to-serine mutants were constructed, which all interfered with the disulfide bond formation of PIF5 to different extents. All the mutants lost their oral infectivity but had no impact on infectious budding virus (BV) production or virus morphogenesis. Taken together, our results indicated PIF5 as the first identified substrate of P33. Further, the disulfide bonds in PIF5 play an essential role in its function in oral infection. IMPORTANCE Similar to some large DNA viruses that encode their own disulfide bond pathway, baculovirus encodes a viral sulfhydryl oxidase, P33. Enzyme activity of P33 is related to infectious BV production, occlusion-derived virus (ODV) envelopment, occlusion body morphogenesis, and oral infectivity, suggesting that P33 is involved in disulfide bond formation of multiple proteins. A complete disulfide bond formation pathway normally contains a sulfhydryl oxidase, a disulfide-donating enzyme, and one or more substrates. In baculovirus, apart from P33, other components of the putative pathway remain unknown. In this study, we identified PIF5 as the first substrate of P33, which is fundamental for revealing the complete disulfide bond formation pathway in baculovirus. PIF5 is essential for oral infection and is absent from the PIF complex. Our study demonstrated that native disulfide bonds in PIF5 are required for oral infection, which will help us to reveal its mode of action.

scholarly journals Disulfide Bond Formation: Sulfhydryl Oxidase ALR Controls Mitochondrial Biogenesis of Human MIA40

Traffic ◽  
2012 ◽  
Vol 14 (3) ◽  
pp. 309-320 ◽  
Author(s):  
Malgorzata E. Sztolsztener ◽  
Anita Brewinska ◽  
Bernard Guiard ◽  
Agnieszka Chacinska
Keyword(s):  
Disulfide Bond ◽  
Mitochondrial Biogenesis ◽  
Bond Formation ◽  
Disulfide Bond Formation ◽  
Sulfhydryl Oxidase

scholarly journals Oxygen-independent disulfide bond formation in VEGF-A and CA9

2021 ◽  
pp. 100505
Author(s):  
Fiana Levitin ◽  
Sandy Che-Eun Serena Lee ◽  
Stephanie Hulme ◽  
Ryan A. Rumantir ◽  
Amy S. Wong ◽  
...  
Keyword(s):  
Disulfide Bond ◽  
Bond Formation ◽  
Disulfide Bond Formation
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