RecA Protein ofMycobacteriumtuberculosisPossesses pH-Dependent Homologous DNA Pairing and Strand Exchange Activities:  Implications for Allele Exchange in Mycobacteria†

Biochemistry ◽  
1999 ◽  
Vol 38 (10) ◽  
pp. 3175-3186 ◽  
Author(s):  
Moreshwar B. Vaze ◽  
K. Muniyappa
Keyword(s):  
Reca Protein ◽  
Strand Exchange ◽  
Ph Dependent ◽  
Dna Pairing

scholarly journals Characterization of an ATP-dependent DNA strand transferase from human cells.

1987 ◽  
Vol 7 (9) ◽  
pp. 3124-3130 ◽  
Author(s):  
D Ganea ◽  
P Moore ◽  
L Chekuri ◽  
R Kucherlapati
Keyword(s):  
Dna Sequences ◽  
Atp Hydrolysis ◽  
Reca Protein ◽  
Strand Exchange ◽  
Cell Nuclei ◽  
In Vitro Recombination ◽  
Dna Strand ◽  
Exchange Activity

We have characterized an enzymatic activity from human cell nuclei which is capable of catalyzing strand exchange between homologous DNA sequences. The strand exchange activity was Mg2+ dependent and required ATP hydrolysis. In addition, it was capable of promoting reannealing of homologous DNA sequences and could form nucleoprotein networks in a fashion reminiscent of purified bacterial RecA protein. Using an in vitro recombination assay, we also showed that the strand exchange activity was biologically important. The factor(s) responsible for the activity has been partially purified.

scholarly journals RecFOR Proteins Load RecA Protein onto Gapped DNA to Accelerate DNA Strand Exchange

2003 ◽  
Vol 11 (5) ◽  
pp. 1337-1347 ◽  
Author(s):  
Katsumi Morimatsu ◽  
Stephen C Kowalczykowski
Keyword(s):  
Reca Protein ◽  
Strand Exchange ◽  
Dna Strand Exchange ◽  
Dna Strand ◽  
Gapped Dna
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