Studying Low-Density Lipoprotein−Monoclonal Antibody Complexes Using Dynamic Laser Light Scattering and Analytical Ultracentrifugation†

Biochemistry ◽  
1999 ◽  
Vol 38 (4) ◽  
pp. 1284-1292 ◽  
Author(s):  
Monica M. Gutierrez ◽  
Shih-wa Tsai ◽  
Martin L. Phillips ◽  
Linda K. Curtiss ◽  
Ross W. Milne ◽  
...  
Keyword(s):  
Monoclonal Antibody ◽  
Light Scattering ◽  
Analytical Ultracentrifugation ◽  
Laser Light ◽  
Low Density Lipoprotein ◽  
Density Lipoprotein ◽  
Laser Light Scattering ◽  
Low Density ◽  
Dynamic Laser Light Scattering

scholarly journals Highly oxidized low-density lipoprotein does not facilitate platelet aggregation

2020 ◽  
Vol 48 (10) ◽  
pp. 030006052095896
Author(s):  
Akari Miyazaki ◽  
Takeshi Uehara ◽  
Yoko Usami ◽  
Nau Ishimine ◽  
Mitsutoshi Sugano ◽  
...  
Keyword(s):  
Light Scattering ◽  
Platelet Aggregation ◽  
Laser Light ◽  
Low Density Lipoprotein ◽  
Density Lipoprotein ◽  
Laser Light Scattering ◽  
Light Transmittance ◽  
Scattering Method ◽  
Low Density ◽  
Oxidized Low Density Lipoprotein

Objective This study aimed to examine whether oxidized low-density lipoprotein (oxLDL) facilitates platelet aggregation, which is one cause for development of cardiovascular disease. Methods The susceptibility of platelets to aggregation was monitored by light transmittance aggregometry and a laser light scattering method using low-density lipoprotein (LDL) and oxLDL as agonists. β-thromboglobulin (β-TG) levels released from platelets were also measured after incubation with or without oxLDL. Results Platelet aggregation was suppressed by oxLDL as estimated by maximum light transmission. Additionally, adenosine diphosphate-induced further aggregation was slightly reduced by the presence of oxLDL. Aggregation levels of a low number of platelets, which was determined by the laser light scattering method, were lower upon addition of oxLDL compared with unoxidized LDL. After a short time of incubation, oxLDL increased secreted β-TG levels in platelet-rich plasma. However, further incubation with oxLDL caused relatively lower secreted β-TG levels compared with incubation with unoxidized LDL. This fluctuation was not due to β-TG degradation by oxLDL. Conclusions Levels of oxLDL in vitro weakly activate platelets at an early stage, but then inhibit platelet function, such as aggregation and β-TG secretion.

A supramolecular ladder polymer prepared by hydrogen bonding-mediated self-assembly of a metallomacrocycle

2015 ◽  
Vol 51 (12) ◽  
pp. 2349-2352 ◽  
Author(s):  
Chun-Fai Ng ◽  
Hak-Fun Chow
Keyword(s):  
Hydrogen Bonding ◽  
Light Scattering ◽  
Self Assembly ◽  
Laser Light ◽  
Laser Light Scattering ◽  
Dynamic Laser Light Scattering ◽  
Ladder Polymer

A defect-free supramolecular ladder polymer was prepared by H-bond-mediated self-assembly of a metallocycle 1 as determined by NMR, viscometry and dynamic laser light scattering studies.

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