Protein-Mediated Inward Translocation of Phospholipids Occurs in both the Apical and Basolateral Plasma Membrane Domains of Epithelial Cells†

Biochemistry ◽  
1999 ◽  
Vol 38 (1) ◽  
pp. 142-150 ◽  
Author(s):  
Thomas Pomorski ◽  
Andreas Herrmann ◽  
Peter Müller ◽  
Gerrit van Meer ◽  
Koert Burger
Keyword(s):  
Plasma Membrane ◽  
Epithelial Cells ◽  
Membrane Domains ◽  
Basolateral Plasma Membrane ◽  
Plasma Membrane Domains

Characterization of Apical and Basolateral Plasma Membrane Domains Derived from Cultured Rat Cholangiocytes

1997 ◽  
Vol 254 (2) ◽  
pp. 192-199 ◽  
Author(s):  
Pamela Tietz ◽  
Susan Levine ◽  
Ralph Holman ◽  
Chris Fretham ◽  
Nicholas F. Larusso
Keyword(s):  
Plasma Membrane ◽  
Membrane Domains ◽  
Basolateral Plasma Membrane ◽  
Plasma Membrane Domains

Isolation and characterization of rat cholangiocyte vesicles enriched in apical or basolateral plasma membrane domains

Biochemistry ◽  
1995 ◽  
Vol 34 (47) ◽  
pp. 15436-15443 ◽  
Author(s):  
Pamela S. Tietz ◽  
Ralph T. Holman ◽  
Laurence J. Miller ◽  
Nicholas F. LaRusso
Keyword(s):  
Plasma Membrane ◽  
Membrane Domains ◽  
Isolation And Characterization ◽  
Basolateral Plasma Membrane ◽  
Plasma Membrane Domains

scholarly journals Epithelial polarity requires septin coupling of vesicle transport to polyglutamylated microtubules

2008 ◽  
Vol 180 (2) ◽  
pp. 295-303 ◽  
Author(s):  
Elias T. Spiliotis ◽  
Stephen J. Hunt ◽  
Qicong Hu ◽  
Makoto Kinoshita ◽  
W. James Nelson
Keyword(s):  
Plasma Membrane ◽  
Epithelial Cells ◽  
Basolateral Membrane ◽  
Vesicle Transport ◽  
Epithelial Polarity ◽  
Membrane Domains ◽  
Polarized Epithelia ◽  
Membrane Growth ◽  
Basolateral Plasma Membrane ◽  
Golgi Network

In epithelial cells, polarized growth and maintenance of apical and basolateral plasma membrane domains depend on protein sorting from the trans-Golgi network (TGN) and vesicle delivery to the plasma membrane. Septins are filamentous GTPases required for polarized membrane growth in budding yeast, but whether they function in epithelial polarity is unknown. Here, we show that in epithelial cells septin 2 (SEPT2) fibers colocalize with a subset of microtubule tracks composed of polyglutamylated (polyGlu) tubulin, and that vesicles containing apical or basolateral proteins exit the TGN along these SEPT2/polyGlu microtubule tracks. Tubulin-associated SEPT2 facilitates vesicle transport by maintaining polyGlu microtubule tracks and impeding tubulin binding of microtubule-associated protein 4 (MAP4). Significantly, this regulatory step is required for polarized, columnar-shaped epithelia biogenesis; upon SEPT2 depletion, cells become short and fibroblast-shaped due to intracellular accumulation of apical and basolateral membrane proteins, and loss of vertically oriented polyGlu microtubules. We suggest that septin coupling of the microtubule cytoskeleton to post-Golgi vesicle transport is required for the morphogenesis of polarized epithelia.

Protein trafficking and polarity in kidney epithelium: from cell biology to physiology

1996 ◽  
Vol 76 (1) ◽  
pp. 245-297 ◽  
Author(s):  
D. Brown ◽  
J. L. Stow
Keyword(s):  
Plasma Membrane ◽  
Epithelial Cells ◽  
Protein Trafficking ◽  
Cell Biology ◽  
Cell Types ◽  
Membrane Domains ◽  
Disease States ◽  
Target Membrane ◽  
Plasma Membrane Domains ◽  
Membrane Components

The transepithelial movement of fluids, electrolytes, and larger molecules is achieved by the activity of a host of specialized transporting proteins, including enzymes, receptors, and channels, that are located on either the apical, basal, or lateral plasma membrane domains of epithelial cells. In the kidney as well as in all other organs, this remarkable polarity of epithelial cells depends on the selective insertion of newly synthesized and recycling proteins and lipids into distinct plasma membrane domains and on the maintenance and modulation of these specialized domains once they are established during epithelial development. This review addresses the mechanisms by which epithelial cells control the movement of membrane components within the cell to ensure that they are delivered to the correct target membrane. Among the topics discussed are targeting signals within membrane proteins, the role of the cytoskeleton and the tight junctional barrier in cell polarity, and the requirement for accessory proteins in the targeting process, including GTP-binding proteins, and proteins that are involved in vesicle docking and fusion events. The final part of the review is devoted uniquely to the polarized targeting of functionally defined proteins in various kidney cell types. In concluding, examples of how a breakdown in these trafficking pathways may be related to some disease states are presented.

Membrane microdomains: lessons from microscopy

1995 ◽  
Vol 53 ◽  
pp. 776-777
Author(s):  
J.M. Robinson ◽  
J.M Oliver
Keyword(s):  
Spatial Distribution ◽  
Plasma Membrane ◽  
Epithelial Cells ◽  
Plasma Membranes ◽  
Cell Types ◽  
Imaging Modalities ◽  
Membrane Microdomains ◽  
Membrane Domains ◽  
Lateral Heterogeneity ◽  
Functional Importance

Specialized regions of plasma membranes displaying lateral heterogeneity are the focus of this Symposium. Specialized membrane domains are known for certain cell types such as differentiated epithelial cells where lateral heterogeneity in lipids and proteins exists between the apical and basolateral portions of the plasma membrane. Lateral heterogeneity and the presence of microdomains in membranes that are uniform in appearance have been more difficult to establish. Nonetheless a number of studies have provided evidence for membrane microdomains and indicated a functional importance for these structures.This symposium will focus on the use of various imaging modalities and related approaches to define membrane microdomains in a number of cell types. The importance of existing as well as emerging imaging technologies for use in the elucidation of membrane microdomains will be highlighted. The organization of membrane microdomains in terms of dimensions and spatial distribution is of considerable interest and will be addressed in this Symposium.

scholarly journals Rapid Nonvesicular Transport of Sterol between the Plasma Membrane Domains of Polarized Hepatic Cells

2002 ◽  
Vol 277 (33) ◽  
pp. 30325-30336
Author(s):  
Daniel Wüstner ◽  
Andreas Herrmann ◽  
Mingming Hao ◽  
Frederick R. Maxfield
Keyword(s):  
Plasma Membrane ◽  
Membrane Domains ◽  
Hepatic Cells ◽  
Plasma Membrane Domains
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