Implication of DNA-Dependent Protein Kinase in an Early, Essential, Local Phosphorylation Event during End-Joining of DNA Double-Strand Breaks in Vitro†

Biochemistry ◽  
1998 ◽  
Vol 37 (27) ◽  
pp. 9827-9835 ◽  
Author(s):  
Xiao-Yan Gu ◽  
Michael A. Weinfeld ◽  
Lawrence F. Povirk
Keyword(s):  
Protein Kinase ◽  
Double Strand Breaks ◽  
Dna Double Strand Breaks ◽  
End Joining ◽  
Dependent Protein Kinase ◽  
Strand Breaks ◽  
Phosphorylation Event ◽  
Dependent Protein

scholarly journals Cell Cycle Dependence of DNA-dependent Protein Kinase Phosphorylation in Response to DNA Double Strand Breaks

2005 ◽  
Vol 280 (15) ◽  
pp. 14709-14715 ◽  
Author(s):  
Benjamin P. C. Chen ◽  
Doug W. Chan ◽  
Junya Kobayashi ◽  
Sandeep Burma ◽  
Aroumougame Asaithamby ◽  
...  
Keyword(s):  
Cell Cycle ◽  
Protein Kinase ◽  
Double Strand Breaks ◽  
Dna Double Strand Breaks ◽  
Dependent Protein Kinase ◽  
Strand Breaks ◽  
Dependent Protein ◽  
Cell Cycle Dependence ◽  
Kinase Phosphorylation ◽  
Cycle Dependence

scholarly journals DNA-dependent Protein Kinase and XRCC4-DNA Ligase IV Mobilization in the Cell in Response to DNA Double Strand Breaks

2004 ◽  
Vol 280 (8) ◽  
pp. 7060-7069 ◽  
Author(s):  
Jérôme Drouet ◽  
Christine Delteil ◽  
Jacques Lefrançois ◽  
Patrick Concannon ◽  
Bernard Salles ◽  
...  
Keyword(s):  
Protein Kinase ◽  
Double Strand Breaks ◽  
Dna Ligase ◽  
Dna Double Strand Breaks ◽  
Dependent Protein Kinase ◽  
Strand Breaks ◽  
Dna Ligase Iv ◽  
Ligase Iv ◽  
Dependent Protein

scholarly journals DNA-dependent protein kinase promotes DNA end processing by MRN and CtIP

2018 ◽  
Author(s):  
Rajashree A. Deshpande ◽  
Logan R. Myler ◽  
Michael M. Soniat ◽  
Nodar Makharashvili ◽  
Linda Lee ◽  
...  
Keyword(s):  
Protein Kinase ◽  
Homologous Recombination ◽  
Dna Double Strand Breaks ◽  
End Joining ◽  
Dependent Protein Kinase ◽  
Strand Breaks ◽  
Mrn Complex ◽  
Dependent Protein ◽  
Non Homologous End Joining ◽  
Dna Ends

AbstractThe repair of DNA double-strand breaks occurs through non-homologous end joining or homologous recombination in vertebrate cells - a choice that is thought to be decided by a competition between DNA-dependent protein kinase (DNA-PK) and the Mre11/Rad50/Nbs1 (MRN) complex but is not well understood. Using ensemble biochemistry and single-molecule approaches, here we show that the MRN complex is dependent on DNA-PK and phosphorylated CtIP to perform efficient processing and resection of DNA ends in physiological conditions, thus eliminating the competition model. Endonucleolytic removal of DNA-PK-bound DNA ends is also observed at double-strand break sites in human cells. The involvement of DNA-PK in MRN-mediated end processing promotes an efficient and sequential transition from non-homologous end joining to homologous recombination by facilitating DNA-PK removal.One Sentence SummaryDNA-dependent protein kinase, an enzyme critical for non-homologous repair of DNA double-strand breaks, also stimulates end processing for homologous recombination.

scholarly journals DNA-Dependent Protein Kinase Catalytic Subunit: The Sensor for DNA Double-Strand Breaks Structurally and Functionally Related to Ataxia Telangiectasia Mutated

Genes ◽  
2021 ◽  
Vol 12 (8) ◽  
pp. 1143
Author(s):  
Yoshihisa Matsumoto ◽  
Anie Day D. C. Asa ◽  
Chaity Modak ◽  
Mikio Shimada
Keyword(s):  
Protein Kinase ◽  
Ataxia Telangiectasia ◽  
Catalytic Subunit ◽  
Nonhomologous End Joining ◽  
Dna Double Strand Breaks ◽  
Ataxia Telangiectasia Mutated ◽  
End Joining ◽  
Dependent Protein Kinase ◽  
Strand Breaks ◽  
Dependent Protein

The DNA-dependent protein kinase (DNA-PK) is composed of a DNA-dependent protein kinase catalytic subunit (DNA-PKcs) and Ku70/Ku80 heterodimer. DNA-PK is thought to act as the “sensor” for DNA double-stranded breaks (DSB), which are considered the most deleterious type of DNA damage. In particular, DNA-PKcs and Ku are shown to be essential for DSB repair through nonhomologous end joining (NHEJ). The phenotypes of animals and human individuals with defective DNA-PKcs or Ku functions indicate their essential roles in these developments, especially in neuronal and immune systems. DNA-PKcs are structurally related to Ataxia–telangiectasia mutated (ATM), which is also implicated in the cellular responses to DSBs. DNA-PKcs and ATM constitute the phosphatidylinositol 3-kinase-like kinases (PIKKs) family with several other molecules. Here, we review the accumulated knowledge on the functions of DNA-PKcs, mainly based on the phenotypes of DNA-PKcs-deficient cells in animals and human individuals, and also discuss its relationship with ATM in the maintenance of genomic stability.

Sign in / Sign up

Export Citation Format

Share Document