Understanding the Role of the Essential Asp251 in Cytochrome P450cam Using Site-Directed Mutagenesis, Crystallography, and Kinetic Solvent Isotope Effect†

Biochemistry ◽  
1998 ◽  
Vol 37 (26) ◽  
pp. 9211-9219 ◽  
Author(s):  
Momcilo Vidakovic ◽  
Stephen G. Sligar ◽  
Huiying Li ◽  
Thomas L. Poulos
Keyword(s):  
Isotope Effect ◽  
Site Directed Mutagenesis ◽  
Directed Mutagenesis ◽  
Cytochrome P450cam ◽  
Solvent Isotope Effect ◽  
Solvent Isotope

SOLVOLYSIS IN LIGHT AND HEAVY WATER: VI. THE ROLE OF INITIAL STATE STRUCTURE

1965 ◽  
Vol 43 (1) ◽  
pp. 154-158 ◽  
Author(s):  
P. M. Laughton ◽  
R. E. Robertson
Keyword(s):  
Isotope Effect ◽  
Heavy Water ◽  
Solubility Data ◽  
State Structure ◽  
Solvent Isotope Effect ◽  
Initial State ◽  
Solvent Isotope

The solubility data in light and heavy water of Swain and Thornton and of Taft and coworkers are reinterpreted to show that they support primarily the initial state origin for the kinetic solvent isotope effect. Other recent data, including the effect for t-butyl and α-phenethyl fluorides, are also shown to be compatible with the same hypothesis.

scholarly journals Site-directed mutagenesis of tyrosine hydroxylase. Role of serine 40 in catalysis.

1992 ◽  
Vol 267 (36) ◽  
pp. 25754-25758
Author(s):  
J Wu ◽  
D Filer ◽  
A.J. Friedhoff ◽  
M Goldstein
Keyword(s):  
Tyrosine Hydroxylase ◽  
Site Directed Mutagenesis ◽  
Directed Mutagenesis

scholarly journals The role of Val68(E11) in ligand binding to sperm whale myoglobin. Site-directed mutagenesis of a synthetic gene.

1990 ◽  
Vol 265 (20) ◽  
pp. 11788-11795
Author(s):  
K D Egeberg ◽  
B A Springer ◽  
S G Sligar ◽  
T E Carver ◽  
R J Rohlfs ◽  
...  
Keyword(s):  
Ligand Binding ◽  
Sperm Whale ◽  
Site Directed Mutagenesis ◽  
Synthetic Gene ◽  
Directed Mutagenesis ◽  
Sperm Whale Myoglobin

Neutral Solvolysis of Acyl Carbon and the Temperature Dependence of the Kinetic Solvent Isotope Effect

1975 ◽  
Vol 53 (6) ◽  
pp. 869-877 ◽  
Author(s):  
B. Rossall ◽  
R. E. Robertson
Keyword(s):  
Temperature Dependence ◽  
Isotope Effect ◽  
Solvent Isotope Effect ◽  
Acyl Carbon ◽  
Rate Of Hydrolysis ◽  
Solvent Isotope ◽  
Neutral Conditions ◽  
Hydrolysis Of

The temperature dependence of the rate of hydrolysis of benzoic, phthalic, and succinic anhydrides have been determined in H2O and D2O under "neutral" conditions. Corresponding data have been obtained for methyl trifluoroacetate. While both series supposedly react by the same BAc2 mechanism, remarkable differences are made obvious by this investigation. Possible sources of such differences are proposed.

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