The 23 and 17 kDa Extrinsic Proteins of Photosystem II Modulate the Magnetic Properties of the S1-State Manganese Cluster†

Biochemistry ◽  
1998 ◽  
Vol 37 (15) ◽  
pp. 5039-5045 ◽  
Author(s):  
Kristy A. Campbell ◽  
Wolfgang Gregor ◽  
Donna P. Pham ◽  
Jeffrey M. Peloquin ◽  
Richard J. Debus ◽  
...  
Keyword(s):  
Magnetic Properties ◽  
Photosystem Ii ◽  
Manganese Cluster ◽  
Extrinsic Proteins

Ligand Environment of the S2State of Photosystem II: A Study of the Hyperfine Interactions of the Tetranuclear Manganese Cluster by 2D14N HYSCORE Spectroscopy

2010 ◽  
Vol 114 (33) ◽  
pp. 10905-10911 ◽  
Author(s):  
Sergey Milikisiyants ◽  
Ruchira Chatterjee ◽  
Amanda Weyers ◽  
Ashley Meenaghan ◽  
Christopher Coates ◽  
...  
Keyword(s):  
Photosystem Ii ◽  
Hyperfine Interactions ◽  
Manganese Cluster ◽  
Ligand Environment

scholarly journals Evidence that D1 Processing Is Required for Manganese Binding and Extrinsic Protein Assembly into Photosystem II

2004 ◽  
Vol 279 (44) ◽  
pp. 45417-45422 ◽  
Author(s):  
Johnna L. Roose ◽  
Himadri B. Pakrasi
Keyword(s):  
Photosystem Ii ◽  
Mutant Cell ◽  
Protein A ◽  
D1 Protein ◽  
Fluorescence Decay ◽  
Normal Function ◽  
Temporal Position ◽  
Decay Kinetics ◽  
Fluorescence Decay Kinetics ◽  
Extrinsic Proteins

Photosystem II (PSII) is a large membrane protein complex that catalyzes oxidation of water to molecular oxygen. During its normal function, PSII is damaged and frequently turned over. The maturation of the D1 protein, a key component in PSII, is a critical step in PSII biogenesis. The precursor form of D1 (pD1) contains a C-terminal extension, which is removed by the protease CtpA to yield PSII complexes with oxygen evolution activity. To determine the temporal position of D1 processing in the PSII assembly pathway, PSII complexes containing only pD1 were isolated from a CtpA-deficient strain of the cyanobacteriumSynechocystis6803. Although membranes from the mutant cell had nearly 50% manganese, no manganese was detected in isolated ΔctpAHT3 PSII, indicating a severely decreased manganese affinity. However, chlorophyll fluorescence decay kinetics after a single saturating flash suggested that the donor YZwas accessible to exogenous Mn2+ions. Furthermore, the extrinsic proteins PsbO, PsbU, and PsbV were not present in PSII isolated from this mutant. However, PsbO and PsbV were present in mutant membranes, but the amount of PsbV protein was consistently less in the mutant membranes compared with the control membranes. We conclude that D1 processing precedes manganese binding and assembly of the extrinsic proteins into PSII. Interestingly, the Psb27 protein was found to be more abundant in ΔctpAHT3 PSII than in HT3 PSII, suggesting a possible role of Psb27 as an assembly factor during PSII biogenesis.

Multifrequency High-Field EPR Study of the Interaction between the Tyrosyl Z Radical and the Manganese Cluster in Plant Photosystem II

1999 ◽  
Vol 103 (49) ◽  
pp. 10945-10954 ◽  
Author(s):  
Pierre Dorlet ◽  
Alain Boussac ◽  
A. William Rutherford ◽  
Sun Un
Keyword(s):  
Photosystem Ii ◽  
High Field ◽  
Manganese Cluster
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