Unique DNA Binding Mode of the N-Terminal Zinc Finger of Transcription Factor Sp1†

Biochemistry ◽  
1998 ◽  
Vol 37 (19) ◽  
pp. 6824-6832 ◽  
Author(s):  
Masanori Yokono ◽  
Nana Saegusa ◽  
Keizo Matsushita ◽  
Yukio Sugiura
Keyword(s):  
Transcription Factor ◽  
Dna Binding ◽  
Zinc Finger ◽  
Binding Mode ◽  
Transcription Factor Sp1

NMR Structure of Transcription Factor Sp1 DNA Binding Domain†,‡

Biochemistry ◽  
2004 ◽  
Vol 43 (51) ◽  
pp. 16027-16035 ◽  
Author(s):  
Shinichiro Oka ◽  
Yasuhisa Shiraishi ◽  
Takuya Yoshida ◽  
Tadayasu Ohkubo ◽  
Yukio Sugiura ◽  
...  
Keyword(s):  
Transcription Factor ◽  
Dna Binding ◽  
Binding Domain ◽  
Nmr Structure ◽  
Dna Binding Domain ◽  
Transcription Factor Sp1

scholarly journals Functional Interaction of the DNA-binding Transcription Factor Sp1 through Its DNA-binding Domain with the Histone Chaperone TAF-I

2003 ◽  
Vol 278 (31) ◽  
pp. 28758-28764 ◽  
Author(s):  
Toru Suzuki ◽  
Shinsuke Muto ◽  
Saku Miyamoto ◽  
Kenichi Aizawa ◽  
Masami Horikoshi ◽  
...  
Keyword(s):  
Transcription Factor ◽  
Dna Binding ◽  
Histone Chaperone ◽  
Binding Domain ◽  
Dna Binding Domain ◽  
Functional Interaction ◽  
Transcription Factor Sp1

The combination of sequence-specific and nonspecific DNA-binding modes of transcription factor SATB1

2016 ◽  
Vol 473 (19) ◽  
pp. 3321-3339 ◽  
Author(s):  
Kazuhiko Yamasaki ◽  
Tomoko Yamasaki
Keyword(s):  
Transcription Factor ◽  
Dna Binding ◽  
Tertiary Structure ◽  
Specific Binding ◽  
Binding Mode ◽  
Matrix Attachment Region ◽  
Titration Calorimetry ◽  
Affinity Constant ◽  
Target Sequence ◽  
Binding Modes

Transcription factor SATB1 (special AT-rich sequence binding protein 1) contains multiple DNA-binding domains (DBDs), i.e. two CUT-domain repeats (CUTr1 and CUTr2 from the N-terminus) and a homeodomain, and binds to the matrix attachment region (MAR) of DNA. Although CUTr1 and the homeodomain, but not CUTr2, are known to contribute to DNA binding, different research groups have not reached a consensus on which DBD is responsible for recognition of the target sequence in MAR, 5′-TAATA-3′. Here, we used isothermal titration calorimetry to demonstrate that CUTr1 has binding specificity to this motif, whereas the homeodomain shows affinity for a variety of DNAs without specificity. In line with nonspecific DNA-binding properties of the homeodomain, a mutation of the invariant Asn at position 51 of the homeodomain (typically in contact with the A base in a sequence-specific binding mode) did not affect the binding affinity significantly. The NMR analyses and computational modeling of the homeodomain, however, revealed the tertiary structure and DNA-binding mode that are typical of homeodomains capable of sequence-specific binding. We believe that the lack of highly conserved basic residues in the helix relevant to the base recognition loosens its fitting into the DNA groove and impairs the specific binding. The two DBDs, when fused in tandem, showed strong binding to DNA containing the 5′-TAATA-3′ motif with an affinity constant >108 M−1 and retained nonspecific binding activity. The combination of the sequence-specific and nonspecific DNA-binding modes of SATB1 should be advantageous in a search for target loci during transcriptional regulation.

scholarly journals Regions outside the DNA-binding domain are critical for proper in vivo specificity of an archetypal zinc finger transcription factor

2013 ◽  
Vol 42 (1) ◽  
pp. 276-289 ◽  
Author(s):  
J. Burdach ◽  
A. P. W. Funnell ◽  
K. S. Mak ◽  
C. M. Artuz ◽  
B. Wienert ◽  
...  
Keyword(s):  
Transcription Factor ◽  
Dna Binding ◽  
Zinc Finger ◽  
Binding Domain ◽  
Dna Binding Domain ◽  
Zinc Finger Transcription Factor

[2,1-c] [1,4]Benzodiazepine (PBD)-Distamycin Hybrid Inhibits DNA Binding to Transcription Factor Sp1

2000 ◽  
Vol 19 (8) ◽  
pp. 1219-1229 ◽  
Author(s):  
P. G. Baraldi ◽  
B. Cacciarit ◽  
A. Guiotto ◽  
R. Romagnoli ◽  
G. Spalluto ◽  
...  
Keyword(s):  
Transcription Factor ◽  
Dna Binding ◽  
Transcription Factor Sp1

scholarly journals The Histone Gene Cell Cycle Regulator HiNF-P Is a Unique Zinc Finger Transcription Factor with a Novel Conserved Auxiliary DNA-Binding Motif†

Biochemistry ◽  
2008 ◽  
Vol 47 (44) ◽  
pp. 11415-11423 ◽  
Author(s):  
Ricardo Medina ◽  
Timothy Buck ◽  
Sayyed K. Zaidi ◽  
Angela Miele-Chamberland ◽  
Jane B. Lian ◽  
...  
Keyword(s):  
Cell Cycle ◽  
Transcription Factor ◽  
Dna Binding ◽  
Zinc Finger ◽  
Histone Gene ◽  
Binding Motif ◽  
Zinc Finger Transcription Factor ◽  
Cell Cycle Regulator
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