Semifunctional Site-Specific Mutants Affecting the Hydrolytic Half-Reaction of Microsomal Epoxide Hydrolase†

Biochemistry ◽  
1998 ◽  
Vol 37 (9) ◽  
pp. 2905-2911 ◽  
Author(s):  
Huey-Fen Tzeng ◽  
L. Timothy Laughlin ◽  
Richard N. Armstrong
Keyword(s):  
Epoxide Hydrolase ◽  
Microsomal Epoxide Hydrolase ◽  
Site Specific

Mechanism of Microsomal Epoxide Hydrolase. Semifunctional Site-Specific Mutants Affecting the Alkylation Half-Reaction†

Biochemistry ◽  
1998 ◽  
Vol 37 (9) ◽  
pp. 2897-2904 ◽  
Author(s):  
L. Timothy Laughlin ◽  
Huey-Fen Tzeng ◽  
Sue Lin ◽  
Richard N. Armstrong
Keyword(s):  
Epoxide Hydrolase ◽  
Microsomal Epoxide Hydrolase ◽  
Site Specific

scholarly journals Human hepatic microsomal epoxide hydrolase. A structural comparison with the rat enzyme.

1982 ◽  
Vol 257 (5) ◽  
pp. 2708-2712
Author(s):  
G C DuBois ◽  
E Appella ◽  
D E Ryan ◽  
D M Jerina ◽  
W Levin
Keyword(s):  
Epoxide Hydrolase ◽  
Microsomal Epoxide Hydrolase ◽  
Structural Comparison

Microsomal epoxide hydrolase and glutathione S-transferase polymorphisms in relation to laryngeal carcinoma risk

2002 ◽  
Vol 187 (1-2) ◽  
pp. 95-101 ◽  
Author(s):  
Jordi To-Figueras ◽  
Manuel Gené ◽  
Jesús Gómez-Catalán ◽  
Esther Piqué ◽  
Natividad Borrego ◽  
...  
Keyword(s):  
Laryngeal Carcinoma ◽  
Epoxide Hydrolase ◽  
Microsomal Epoxide Hydrolase ◽  
Glutathione S Transferase ◽  
Carcinoma Risk

Enhancement of Radiation-Induced Hepatic Microsomal Epoxide Hydrolase Gene Expression by Oltipraz in Rats

1997 ◽  
Vol 147 (5) ◽  
pp. 613 ◽  
Author(s):  
Seon Young Nam ◽  
Jeong Hee Kim ◽  
Chul Koo Cho ◽  
Seong Yul Yoo ◽  
Sang Geon Kim
Keyword(s):  
Gene Expression ◽  
Epoxide Hydrolase ◽  
Microsomal Epoxide Hydrolase ◽  
Radiation Induced

Association of microsomal epoxide hydrolase gene (fast genotype) with lung functions impairment in wood workers

2021 ◽  
Vol 0 (0) ◽  
Author(s):  
Mona M. Taha ◽  
Amal Saad-Hussein ◽  
Heba Mahdy-Abdallah
Keyword(s):  
Epoxide Hydrolase ◽  
Fine Particles ◽  
Forced Expiratory Volume ◽  
Significant Rise ◽  
Lung Epithelial Cells ◽  
Wood Dust ◽  
Microsomal Epoxide Hydrolase ◽  
Lung Functions ◽  
Exon 4

Abstract Objectives Exposure to wood dust may lead to impairment of the lung functions. Microsomal epoxide hydrolase enzyme (EPHX1) was shown to take part in protection against oxidative stress. An alteration in enzyme activity might be associated with its gene polymorphisms. In vitro polymorphisms in exons 3 (His113Tyr) and 4 (Arg139His) lead to reduced activity (slow allele) and increased activity (fast allele). Macrophage inflammatory protein 2 (MIP-2) is produced in rat lung epithelial cells after exposure to fine particles. We aimed to investigate the associations between mEPHX1 polymorphisms (in exon 3 and 4) and lung function in furniture workers and assessment of MIP-2 effect. Methods Our study was performed on 70 wood dust exposed male workers and 70 matched normal controls subjects. Ventilatory function tests were measured by spirometer, MIP-2 was performed by ELISA methods and EPHX gene was done by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) methods for each participant. Results Significant reduction in forced vital capacity (FVC%) and forced expiratory volume in the first second (FEV1) levels in Tyr–Tyr and Tyr–Hist genotypes of EPHX (exon 3) was observed. Reduced peak expiratory flow (PEF) levels and significant rise in MIP-2 levels were detected in Tyr–Tyr genotype. While high significant reduction in FVC% and FEV1 levels were shown in different genotypes in exon 4. Significant rise was observed in MIP-2 levels in Hist–Hist genotype of exon 4. An increase in duration of exposure showed positive correlation with fall in ventilatory functions. Conclusions It was concluded that in Hist139Arg of EPHX gene, fast genotype (Arg–Arg) was associated with impaired ventilatory functions.

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