Bovine Epidermal Fatty Acid-Binding Protein:  Determination of Ligand Specificity and Cellular Localization in Retina and Testis†

Biochemistry ◽  
1998 ◽  
Vol 37 (10) ◽  
pp. 3250-3257 ◽  
Author(s):  
Pamela B. Kingma ◽  
Dean Bok ◽  
David E. Ong
Keyword(s):  
Fatty Acid ◽  
Cellular Localization ◽  
Fatty Acid Binding Protein ◽  
Binding Protein ◽  
Ligand Specificity ◽  
Protein Determination ◽  
Fatty Acid Binding ◽  
Acid Binding Protein

Detection, tissue distribution and (sub)cellular localization of fatty acid-binding protein types

1990 ◽  
pp. 11-18 ◽  
Author(s):  
J. H. Veerkamp ◽  
R. J. A. Paulussen ◽  
R. A. Peeters ◽  
R. G. H. J. Maatman ◽  
H. T. B. van Moerkerk ◽  
...  
Keyword(s):  
Fatty Acid ◽  
Tissue Distribution ◽  
Cellular Localization ◽  
Fatty Acid Binding Protein ◽  
Binding Protein ◽  
Fatty Acid Binding ◽  
Acid Binding Protein

Fatty acid binding protein 7 expression and its sub-cellular localization in breast cancer

2012 ◽  
Vol 134 (2) ◽  
pp. 519-529 ◽  
Author(s):  
Alaa T. Alshareeda ◽  
Emad A. Rakha ◽  
Christopher C. Nolan ◽  
Ian O. Ellis ◽  
Andrew R. Green
Keyword(s):  
Breast Cancer ◽  
Fatty Acid ◽  
Cellular Localization ◽  
Fatty Acid Binding Protein ◽  
Binding Protein ◽  
Fatty Acid Binding ◽  
Acid Binding Protein

scholarly journals A Point-of-Care Immunotest for Bedside Determination of Heart-Type Fatty Acid-Binding Protein

2010 ◽  
Vol 43 (4) ◽  
pp. 735-744 ◽  
Author(s):  
Chao-Yang Tong ◽  
Cangel Pui-Yee Chan ◽  
Kwan-Yee Cheung ◽  
George W. H. Cautherley ◽  
Jan F. C. Glatz ◽  
...  
Keyword(s):  
Fatty Acid ◽  
Fatty Acid Binding Protein ◽  
Binding Protein ◽  
Point Of Care ◽  
Fatty Acid Binding ◽  
Acid Binding Protein

scholarly journals Mutations of recombinant rat liver fatty acid-binding protein at residues 102 and 122 alter its structural integrity and affinity for physiological ligands

1996 ◽  
Vol 314 (3) ◽  
pp. 943-949 ◽  
Author(s):  
Alfred E. A. THUMSER ◽  
Joanne VOYSEY ◽  
David C. WILTON
Keyword(s):  
Fatty Acid ◽  
Rat Liver ◽  
Fatty Acid Binding Protein ◽  
Binding Protein ◽  
Ligand Specificity ◽  
Liver Fatty Acid ◽  
Fatty Acid Binding ◽  
Acid Binding Protein ◽  
Arginine Residues ◽  
Portal Region

Rat liver fatty acid-binding protein (FABP) is able to accommodate a wide range of non-polar anions in addition to long-chain fatty acids. This property means that the liver protein is functionally different from other FABPs from intestine, muscle and adipose tissue that have a more restricted ligand specificity and stoichiometry. The availability of crystal structures for the latter proteins has highlighted the importance of two arginine residues that are involved in the binding of the fatty acid carboxylate. Only one of these arginine residues, arginine-122, is conserved in liver FABP, whereas the other arginine, at position 102, is replaced by a threonine. In order to gain further insight into the nature of ligand interactions with liver FABP these key residues (102 and 122) have been changed by site-directed mutagenesis. The results with an R122Q mutant highlight the critical role of this arginine in determining ligand affinity, while similar but less dramatic effects were observed with the T102Q mutant. The double mutant T102Q/R122Q was expressed but had lost the ability to bind fluorescent ligands. It is concluded that Arg-122 plays a role in accommodating the carboxylate group of at least one fatty acid. It is proposed that physiological ligands with more bulky headgroups, such as lysophospholipids, acyl-CoA and mono-olein, bind with the headgroups in a solvent-exposed location near the portal region of the protein. The portal region is suggested to be more flexible in the mutants (R122Q and to a lesser extent T102Q). The net result is that the ligand specificity of the R122Q mutant changes to that of a protein with enhanced affinity for acyl-CoA, lysophospholipids and mono-olein.

Expression, Purification, and Crystal Structure Determination of Recombinant Human Epidermal-Type Fatty Acid Binding Protein†,‡

Biochemistry ◽  
1999 ◽  
Vol 38 (38) ◽  
pp. 12229-12239 ◽  
Author(s):  
Carsten Hohoff ◽  
Torsten Börchers ◽  
Bernd Rüstow ◽  
Friedrich Spener ◽  
Herman van Tilbeurgh
Keyword(s):  
Crystal Structure ◽  
Fatty Acid ◽  
Structure Determination ◽  
Fatty Acid Binding Protein ◽  
Binding Protein ◽  
Crystal Structure Determination ◽  
Fatty Acid Binding ◽  
Acid Binding Protein
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