Evaluation of the Role of Specific Acidic Amino Acid Residues in Electron Transfer between the Flavodoxin and Cytochromec3fromDesulfovibrio vulgaris[Hildenborough]†

Biochemistry ◽  
1997 ◽  
Vol 36 (44) ◽  
pp. 13617-13628 ◽  
Author(s):  
Yucheng Feng ◽  
Richard P. Swenson
Keyword(s):  
Electron Transfer ◽  
Amino Acid ◽  
Amino Acid Residues ◽  
Acidic Amino Acid

scholarly journals Role of Integrase in Reverse Transcription of the Saccharomyces cerevisiae Retrotransposon Ty1

2005 ◽  
Vol 4 (6) ◽  
pp. 1057-1065 ◽  
Author(s):  
M. Wilhelm ◽  
F.-X. Wilhelm
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Amino Acid ◽  
Specific Activity ◽  
Rnase H ◽  
Amino Acid Residues ◽  
Acidic Amino Acid ◽  
Acidic Domain ◽  
Basic Region

ABSTRACT Reverse transcriptase (RT) with its associated RNase H (RH) domain and integrase (IN) are key enzymes encoded by retroviruses and retrotransposons. Several studies have implied a functional role of the interaction between IN and RT during the replication of retroviral and retrotransposon genomes. In this study, IN deletion mutants were used to investigate the role of IN on the RT activity of the yeast Saccharomyces cerevisiae retrotransposon Ty1. We have identified two domains of Ty1 integrase which have effects on RT activity in vivo. The deletion of a domain spanning amino acid residues 233 to 520 of IN increases the exogenous specific activity of RT up to 20-fold, whereas the removal of a region rich in acidic amino acid residues between residues 521 and 607 decreases its activity. The last result complements our observation that an active recombinant RT protein can be obtained if a small acidic tail mimicking the acidic domain of IN is fused to the RT-RH domain. We suggest that interaction between these acidic amino acid residues of IN and a basic region of RT could be critical for the correct folding of RT and for the formation of an active conformation of the enzyme.

Electron Transfer Reaction of Glucose Oxidase Hybrids Modified with PhenothiazineviaPoly(ethylene oxide) Spacer on Acidic Amino Acid Residues

2002 ◽  
Vol 31 (2) ◽  
pp. 256-257 ◽  
Author(s):  
Sayuri Aoki ◽  
Kunikazu Ishii ◽  
Takeshi Ueki ◽  
Kazumichi Ban ◽  
Shin-ichiro Imabayashi ◽  
...  
Keyword(s):  
Electron Transfer ◽  
Amino Acid ◽  
Ethylene Oxide ◽  
Glucose Oxidase ◽  
Transfer Reaction ◽  
Electron Transfer Reaction ◽  
Amino Acid Residues ◽  
Acidic Amino Acid

scholarly journals Local stability identification and the role of key acidic amino acid residues in staphylococcal nuclease unfolding

FEBS Journal ◽  
2005 ◽  
Vol 272 (15) ◽  
pp. 3967-3974 ◽  
Author(s):  
Hueih-Min Chen ◽  
Siu-Chiu Chan ◽  
King-Wong Leung ◽  
Jiun-Ming Wu ◽  
Huey-Jen Fang ◽  
...  
Keyword(s):  
Amino Acid ◽  
Local Stability ◽  
Staphylococcal Nuclease ◽  
Amino Acid Residues ◽  
Acidic Amino Acid

The Role of Acidic Amino Acid Residues in the Structural Stability of Snake Cardiotoxins†

Biochemistry ◽  
1996 ◽  
Vol 35 (28) ◽  
pp. 9177-9186 ◽  
Author(s):  
Chien-Min Chiang ◽  
Shou-Lin Chang ◽  
Hai-jui Lin ◽  
Wen-guey Wu
Keyword(s):  
Amino Acid ◽  
Structural Stability ◽  
Amino Acid Residues ◽  
Acidic Amino Acid

Role of acidic amino acid residues in chitooligosaccharide-binding to Streptomyces sp. N174 chitosanase

2005 ◽  
Vol 338 (4) ◽  
pp. 1839-1844 ◽  
Author(s):  
Tomomi Katsumi ◽  
Marie-Ève Lacombe-Harvey ◽  
Hugo Tremblay ◽  
Ryszard Brzezinski ◽  
Tamo Fukamizo
Keyword(s):  
Amino Acid ◽  
Amino Acid Residues ◽  
Acidic Amino Acid ◽  
Streptomyces Sp

scholarly journals Role of lysine and acidic amino acid residues on the insecticidal activity of Jackbean urease

Toxicon ◽  
2013 ◽  
Vol 71 ◽  
pp. 76-83 ◽  
Author(s):  
Rafael Real-Guerra ◽  
Célia Regina Carlini ◽  
Fernanda Stanisçuaski
Keyword(s):  
Amino Acid ◽  
Insecticidal Activity ◽  
Amino Acid Residues ◽  
Acidic Amino Acid
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