Histidine → Carboxamide Ligand Substitutions in the Zinc Binding Site of Carbonic Anhydrase II Alter Metal Coordination Geometry but Retain Catalytic Activity†

Biochemistry ◽  
1997 ◽  
Vol 36 (50) ◽  
pp. 15780-15791 ◽  
Author(s):  
Charles A. Lesburg ◽  
Chih-chin Huang ◽  
David W. Christianson ◽  
Carol A. Fierke
Keyword(s):  
Catalytic Activity ◽  
Carbonic Anhydrase ◽  
Binding Site ◽  
Zinc Binding ◽  
Metal Coordination ◽  
Carbonic Anhydrase Ii ◽  
Coordination Geometry ◽  
Zinc Binding Site

Effect of the hydrogen bond network in carbonic anhydrase II zinc binding site. A theoretical study

1998 ◽  
Vol 76 (7) ◽  
pp. 1027-1032 ◽  
Author(s):  
Silvia Álvarez-Santos ◽  
Àngels González-Lafont ◽  
José M Lluch
Keyword(s):  
Hydrogen Bond ◽  
Carbonic Anhydrase ◽  
Binding Site ◽  
Zinc Binding ◽  
Carbonic Anhydrase Ii ◽  
Am1 Method ◽  
Hydrogen Bond Network ◽  
Zinc Binding Site ◽  
Bond Network ◽  
Metal Ligand

The hydrogen bond network influence on the carbonic anhydrase II (CAII) zinc binding site has been studied theoretically by using the semiempirical AM1 method. To this aim, quantum mechanical reduced models of wild-type CAII and several CAII variants have been constructed. We have shown that, when a direct metal ligand donates a hydrogen bond to an indirect metal ligand, the first-shell residues enhance their electrostatic interaction with the zinc cation. Thus, the hydrogen-bond network is able to modulate the zinc binding affinity and the zinc-water pKa.Key words: hydrogen bond network, carbonic anhydrase II, Zn2+ metalloenzyme ligands.

Engineering a cysteine ligand into the zinc binding site of human carbonic anhydrase II

Biochemistry ◽  
1993 ◽  
Vol 32 (38) ◽  
pp. 9896-9900 ◽  
Author(s):  
Laura L. Kiefer ◽  
Joseph F. Krebs ◽  
Steven A. Paterno ◽  
Carol A. Fierke
Keyword(s):  
Carbonic Anhydrase ◽  
Binding Site ◽  
Zinc Binding ◽  
Carbonic Anhydrase Ii ◽  
Zinc Binding Site ◽  
Human Carbonic Anhydrase

Structure of His94→Asp carbonic anhydrase II in a new crystalline form reveals a partially occupied zinc binding site

1995 ◽  
Vol 8 (10) ◽  
pp. 975-980 ◽  
Author(s):  
Joseph A. Ippolito ◽  
Satish K. Nair ◽  
Richard S. Alexander ◽  
Laura L. Kiefer ◽  
Carol A. Fierke ◽  
...  
Keyword(s):  
Carbonic Anhydrase ◽  
Binding Site ◽  
Crystalline Form ◽  
Zinc Binding ◽  
Carbonic Anhydrase Ii ◽  
Zinc Binding Site

Structural analysis of the non-catalytic zinc binding site in the minor β-carbonic anhydrase CafD

2019 ◽  
Vol 7 (4) ◽  
pp. 84-89
Author(s):  
Songwon Kim ◽  
Na Jin Kim ◽  
Subin Kim ◽  
Semi Hong ◽  
Jongmin Sung ◽  
...  
Keyword(s):  
Structural Analysis ◽  
Carbonic Anhydrase ◽  
Binding Site ◽  
Zinc Binding ◽  
Zinc Binding Site ◽  
Catalytic Zinc

Crystal Structure of a Zinc-Activated Variant of Human Carbonic Anhydrase I, CA I Michigan 1:  Evidence for a Second Zinc Binding Site Involving Arginine Coordination

Biochemistry ◽  
2002 ◽  
Vol 41 (20) ◽  
pp. 6237-6244 ◽  
Author(s):  
Marta Ferraroni ◽  
Silvia Tilli ◽  
Fabrizio Briganti ◽  
W. Richard Chegwidden ◽  
Claudiu T. Supuran ◽  
...  
Keyword(s):  
Crystal Structure ◽  
Carbonic Anhydrase ◽  
Binding Site ◽  
Zinc Binding ◽  
Carbonic Anhydrase I ◽  
Zinc Binding Site ◽  
Human Carbonic Anhydrase
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