Kinetics of Myosin Subfragment-1-Induced Condensation of G-Actin into Oligomers, Precursors in the Assembly of F-Actin−S1. Role of the Tightly Bound Metal Ion and ATP Hydrolysis†

Biochemistry ◽  
1997 ◽  
Vol 36 (39) ◽  
pp. 11837-11842 ◽  
Author(s):  
Stéphane Fievez ◽  
Dominique Pantaloni ◽  
Marie-France Carlier
Keyword(s):  
Metal Ion ◽  
Atp Hydrolysis ◽  
Myosin Subfragment ◽  
Myosin Subfragment 1 ◽  
Kinetics Of

Nitrogenase of Klebsiella pneumoniae : an MgATP hydrolysing energy transduction system with similarities to actomyosin and p21ras

1992 ◽  
Vol 336 (1276) ◽  
pp. 73-82 ◽  
Keyword(s):  
Metal Ion ◽  
Atp Hydrolysis ◽  
Energy Transduction ◽  
Gtp Hydrolysis ◽  
Kinetic And Thermodynamic Parameters ◽  
Myosin Subfragment ◽  
Myosin Subfragment 1 ◽  
Induced Changes ◽  
Slow Dissociation ◽  
Metal Ion Activation

The mechanism of ATP hydrolysis by nitrogenase shows some similarity to that proposed for actomyosin and for GTP hydrolysis by p21ras. All three systems involve the formation of an active complex from two component proteins, nucleotide-induced changes in protein conformation, energy transduction that in the case of nitrogenase involves a decrease in redox potential of metal centres, and a slow dissociation of the protein complex. Metal ion activation (Mg 2+ or Ca 2+ ) and in-line displacement of ADP by H 2 O without enzyme phosphorylation are also common features. At 5°C, stopped-flow calorimetry shows that the kinetic and thermodynamic parameters for endothermic, reversible on-enzyme cleavage of MgATP by nitrogenase and myosin subfragment 1 are remarkably similar. [ 18 O 4 ]P i -water exchange studies also show that ATP cleavage on nitrogenase and myosin are reversible.

The ATPase kinetics of insect fibrillar flight muscle myosin subfragment-1

1986 ◽  
Vol 7 (2) ◽  
pp. 179-192 ◽  
Author(s):  
D. C. S. White ◽  
R. W. Zimmermann ◽  
D. R. Trentham
Keyword(s):  
Flight Muscle ◽  
Myosin Subfragment ◽  
Myosin Subfragment 1 ◽  
Muscle Myosin ◽  
Kinetics Of

LC20 and kinetics of gizzard myosin subfragment-1: Digestion with papain vs.S. aureus protease

1992 ◽  
Vol 23 (3) ◽  
pp. 213-221 ◽  
Author(s):  
Jean S. Drew ◽  
Marianne P. White ◽  
Carl Moos ◽  
Leonard A. Stein
Keyword(s):  
Myosin Subfragment ◽  
Myosin Subfragment 1 ◽  
Kinetics Of

scholarly journals Kinetics of the Interaction of Myosin Subfragment-1 with G-Actin

1995 ◽  
Vol 270 (13) ◽  
pp. 7125-7133 ◽  
Author(s):  
Laurent Blanchoin ◽  
Stéphane Fievez ◽  
Franck Travers ◽  
Marie-France Carlier ◽  
Dominique Pantaloni
Keyword(s):  
Myosin Subfragment ◽  
Myosin Subfragment 1 ◽  
Kinetics Of

scholarly journals The Role of Magnesium in Binding of the Nucleotide Polyphosphate Chain to the Active Site of Myosin Subfragment-1

1983 ◽  
Vol 134 (2) ◽  
pp. 197-204 ◽  
Author(s):  
Marcus C. SCHAUB ◽  
John G. WATTERSON ◽  
Klaus LOTH ◽  
Daniela FOLETTA
Keyword(s):  
Active Site ◽  
Myosin Subfragment ◽  
Myosin Subfragment 1
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