Binding of the Fluorescein Derivative Eosin Y to the Mitochondrial ADP/ATP Carrier:  Characterization of the Adenine Nucleotide Binding Site†

Biochemistry ◽  
1998 ◽  
Vol 37 (1) ◽  
pp. 424-432 ◽  
Author(s):  
Eiji Majima ◽  
Nana Yamaguchi ◽  
Hiroshi Chuman ◽  
Yasuo Shinohara ◽  
Mayumi Ishida ◽  
...  
Keyword(s):  
Binding Site ◽  
Adenine Nucleotide ◽  
Nucleotide Binding ◽  
Nucleotide Binding Site ◽  
Eosin Y

Characterization of Nucleotide Binding ­Site-Encoding Genes in Sweetpotato, Ipomoea batatas(L.) Lam., and Their Response to Biotic and Abiotic Stresses

2021 ◽  
pp. 1-15
Author(s):  
Zengzhi Si ◽  
Yake Qiao ◽  
Kai Zhang ◽  
Zhixin Ji ◽  
Jinling Han
Keyword(s):  
Binding Site ◽  
Abiotic Stresses ◽  
Ipomoea Batatas ◽  
Expression Profiles ◽  
Nucleotide Binding ◽  
Regulatory Elements ◽  
Nucleotide Binding Site ◽  
Biotic And Abiotic Stresses ◽  
Encoding Genes

Sweetpotato, <i>Ipomoea batatas</i> (L.) Lam., is an important and widely grown crop, yet its production is affected severely by biotic and abiotic stresses. The nucleotide binding site (NBS)-encoding genes have been shown to improve stress tolerance in several plant species. However, the characterization of NBS-encoding genes in sweetpotato is not well-documented to date. In this study, a comprehensive analysis of NBS-encoding genes has been conducted on this species by using bioinformatics and molecular biology methods. A total of 315 NBS-encoding genes were identified, and 260 of them contained all essential conserved domains while 55 genes were truncated. Based on domain architectures, the 260 NBS-encoding genes were grouped into 6 distinct categories. Phylogenetic analysis grouped these genes into 3 classes: TIR, CC (I), and CC (II). Chromosome location analysis revealed that the distribution of NBS-encoding genes in chromosomes was uneven, with a number ranging from 1 to 34. Multiple stress-related regulatory elements were detected in the promoters, and the NBS-encoding genes’ expression profiles under biotic and abiotic stresses were obtained. According to the bioinformatics analysis, 9 genes were selected for RT-qPCR analysis. The results revealed that <i>IbNBS75</i>, <i>IbNBS219</i>, and <i>IbNBS256</i> respond to stem nematode infection; <i>Ib­NBS240</i>, <i>IbNBS90</i>, and <i>IbNBS80</i> respond to cold stress, while <i>IbNBS208</i>, <i>IbNBS71</i>, and <i>IbNBS159</i> respond to 30% PEG treatment. We hope these results will provide new insights into the evolution of NBS-encoding genes in the sweetpotato genome and contribute to the molecular breeding of sweetpotato in the future.

Characterization of resistance gene analogs with a nucleotide binding site isolated from a triploid white poplar

Plant Biology ◽  
2008 ◽  
Vol 10 (3) ◽  
pp. 310-322 ◽  
Author(s):  
Q. Zhang ◽  
Z.-Y. Zhang ◽  
S.-Z. Lin ◽  
H.-Q. Zheng ◽  
Y.-Z. Lin ◽  
...  
Keyword(s):  
Binding Site ◽  
Resistance Gene ◽  
Nucleotide Binding ◽  
Nucleotide Binding Site ◽  
Resistance Gene Analogs ◽  
White Poplar

scholarly journals 2.9 Å crystal structure of ligand-free tryptophanyl-tRNA synthetase: Domain movements fragment the adenine nucleotide binding site

2008 ◽  
Vol 9 (2) ◽  
pp. 218-231 ◽  
Author(s):  
Valentin A. Ilyin ◽  
Brenda Temple ◽  
Mei Hu ◽  
Charles W. Carter ◽  
Genpei Li ◽  
...  
Keyword(s):  
Crystal Structure ◽  
Binding Site ◽  
Adenine Nucleotide ◽  
Nucleotide Binding ◽  
Trna Synthetase ◽  
Nucleotide Binding Site ◽  
Ligand Free

scholarly journals Identification and Characterization of Nucleotide-Binding Site-Leucine-Rich Repeat Genes in the Model Plant Medicago truncatula

2007 ◽  
Vol 146 (1) ◽  
pp. 5-21 ◽  
Author(s):  
Carine Ameline-Torregrosa ◽  
Bing-Bing Wang ◽  
Majesta S. O'Bleness ◽  
Shweta Deshpande ◽  
Hongyan Zhu ◽  
...  
Keyword(s):  
Binding Site ◽  
Medicago Truncatula ◽  
Nucleotide Binding ◽  
Nucleotide Binding Site ◽  
Leucine Rich Repeat ◽  
Model Plant ◽  
Identification And Characterization
Sign in / Sign up

Export Citation Format

Share Document