Comparison of NH Exchange and Circular Dichroism as Techniques for Measuring the Parameters of the Helix−Coil Transition in Peptides†

Biochemistry ◽  
1997 ◽  
Vol 36 (28) ◽  
pp. 8435-8442 ◽  
Author(s):  
Carol A. Rohl ◽  
Robert L. Baldwin
Keyword(s):  
Circular Dichroism ◽  
Coil Transition ◽  
Circular Dichroïsm

Circular dichroism and the pH-induced ?-coil transition of poly(S-carboxymethyl-L-cysteine) and its side-chain homolog

Biopolymers ◽  
1979 ◽  
Vol 18 (2) ◽  
pp. 335-349 ◽  
Author(s):  
Shoichi Ikeda ◽  
Akira Fukutome ◽  
Toyoko Imae ◽  
Tomohiko Yoshida
Keyword(s):  
Circular Dichroism ◽  
Side Chain ◽  
Coil Transition ◽  
Circular Dichroïsm

Synthesis and study of the conformational stability of sequential polypeptides containing δ-benzyloxycarbonyl-L-ornithine and benzyl esters of L-aspartic and L-glutamic acids

1978 ◽  
Vol 56 (5) ◽  
pp. 622-627 ◽  
Author(s):  
Nga Ho-Duc ◽  
Hubert Daoust ◽  
Serge St-Pierre
Keyword(s):  
Circular Dichroism ◽  
Conformational Stability ◽  
Helical Conformation ◽  
Dilute Solution ◽  
Coil Transition ◽  
Solution Methods ◽  
Benzyl Esters ◽  
Circular Dichroïsm

The synthesis of the sequential polypeptides (N-δ-Z-L-Orn-γ-BzI-L-Glu)n and (N-δ-Z-L-Orn-β-BzI-L-Asp)n by solution methods is described. Both polypeptides are α helical in chloroform as shown by circular dichroism. However, the conformation in this solvent is rather fragile, since small additions of TFA to a dilute solution of the polypeptides in chloroform is sufficient to cause the helix-to-coil transition, as demonstrated by cd and nmr. It is postulated that the low stability of the helical conformation is attributed, in this case, to a cooperative collapse of the ordered conformation.

Characterization of Tamm-Horsfall Urinary Glycoprotein

1973 ◽  
Vol 31 ◽  
pp. 420-421
Author(s):  
John P. Robinson ◽  
J. David Puett
Keyword(s):  
Electron Microscopy ◽  
Circular Dichroism ◽  
Secondary Structure ◽  
Quaternary Structure ◽  
Normal Adult ◽  
Morphological Properties ◽  
Adult Males ◽  
Circular Dichroïsm ◽  
Urinary Glycoprotein

Much work has been reported on the chemical, physical and morphological properties of urinary Tamm-Horsfall glycoprotein (THG). Although it was once reported that cystic fibrotic (CF) individuals had a defective THG, more recent data indicate that THG and CF-THG are similar if not identical.No studies on the conformational aspects have been reported on this glycoprotein using circular dichroism (CD). We examined the secondary structure of THG and derivatives under various conditions and have correlated these results with quaternary structure using electron microscopy.THG was prepared from normal adult males and CF-THG from a 16-year old CF female by the method of Tamm and Horsfall. CF female by the method of Tamm and Horsfall.

Circular dichroism spectra of adsorbed dye-aggregates

1968 ◽  
Vol 65 ◽  
pp. 146-151 ◽  
Author(s):  
G. Scheibe ◽  
O. Wörz ◽  
F. Haimerl ◽  
W. Seiffert ◽  
J. Winkler
Keyword(s):  
Circular Dichroism ◽  
Circular Dichroism Spectra ◽  
Circular Dichroïsm
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