Role of Protein−Protein Interactions in the Function of Replication Protein A (RPA):  RPA Modulates the Activity of DNA Polymerase α by Multiple Mechanisms

Biochemistry ◽  
1997 ◽  
Vol 36 (28) ◽  
pp. 8443-8454 ◽  
Author(s):  
Katherine A. Braun ◽  
Ye Lao ◽  
Zhigang He ◽  
C. James Ingles ◽  
Marc S. Wold
Keyword(s):  
Dna Polymerase ◽  
Protein Interactions ◽  
Protein A ◽  
Replication Protein A ◽  
Replication Protein ◽  
Protein Protein Interactions ◽  
Dna Polymerase Α

scholarly journals Replication Protein A as a “Fidelity Clamp” for DNA Polymerase α

2001 ◽  
Vol 276 (21) ◽  
pp. 18235-18242 ◽  
Author(s):  
Giovanni Maga ◽  
Isabelle Frouin ◽  
Silvio Spadari ◽  
Ulrich Hübscher
Keyword(s):  
Dna Polymerase ◽  
Protein A ◽  
Replication Protein A ◽  
Replication Protein ◽  
Dna Polymerase Α

scholarly journals Discovery of a Potent Inhibitor of Replication Protein A Protein–Protein Interactions Using a Fragment-Linking Approach

2013 ◽  
Vol 56 (22) ◽  
pp. 9242-9250 ◽  
Author(s):  
Andreas O. Frank ◽  
Michael D. Feldkamp ◽  
J. Phillip Kennedy ◽  
Alex G. Waterson ◽  
Nicholas F. Pelz ◽  
...  
Keyword(s):  
Protein Interactions ◽  
Potent Inhibitor ◽  
Protein A ◽  
Replication Protein A ◽  
Replication Protein ◽  
Protein Protein Interactions

Abstract 2473: Fragment-based discovery of inhibitors of replication protein A protein-protein interactions.

2013 ◽  
Author(s):  
Alex G. Waterson ◽  
James D. Patrone ◽  
J. Phillip Kennedy ◽  
Nicholas F. Pelz ◽  
Andreas O. Frank ◽  
...  
Keyword(s):  
Protein Interactions ◽  
Protein A ◽  
Replication Protein A ◽  
Replication Protein ◽  
Protein Protein Interactions

scholarly journals Assembly of a Complex Containing Cdc45p, Replication Protein A, and Mcm2p at Replication Origins Controlled by S-Phase Cyclin-Dependent Kinases and Cdc7p-Dbf4p Kinase

2000 ◽  
Vol 20 (9) ◽  
pp. 3086-3096 ◽  
Author(s):  
Lee Zou ◽  
Bruce Stillman
Keyword(s):  
Dna Polymerase ◽  
Origin Recognition Complex ◽  
Protein A ◽  
Replication Protein A ◽  
S Phase ◽  
Replication Protein ◽  
Dependent Manner ◽  
Replication Origins ◽  
Cyclin Dependent Kinases ◽  
Origin Activation

ABSTRACT In Saccharomyces cerevisiae, replication origins are activated with characteristic timing during S phase. S-phase cyclin-dependent kinases (S-CDKs) and Cdc7p-Dbf4p kinase are required for origin activation throughout S phase. The activation of S-CDKs leads to association of Cdc45p with chromatin, raising the possibility that Cdc45p defines the assembly of a new complex at each origin. Here we show that both Cdc45p and replication protein A (RPA) bind to Mcm2p at the G1-S transition in an S-CDK-dependent manner. During S phase, Cdc45p associates with different replication origins at specific times. The origin associations of Cdc45p and RPA are mutually dependent, and both S-CDKs and Cdc7p-Dbf4p are required for efficient binding of Cdc45p to origins. These findings suggest that S-CDKs and Cdc7p-Dbf4p promote loading of Cdc45p and RPA onto a preformed prereplication complex at each origin with preprogrammed timing. TheARS1 association of Mcm2p, but not that of the origin recognition complex, is diminished by disruption of the B2 element ofARS1, a potential origin DNA-unwinding element. Cdc45p is required for recruiting DNA polymerase α onto chromatin, and it associates with Mcm2p, RPA, and DNA polymerase ɛ only during S phase. These results suggest that the complex containing Cdc45p, RPA, and MCMs is involved in origin unwinding and assembly of replication forks at each origin.

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