Spin-Label Studies on the Anchoring and Lipid−Protein Interactions of Avidin withN-Biotinylphosphatidylethanolamines in Lipid Bilayer Membranes

Biochemistry ◽  
1997 ◽  
Vol 36 (24) ◽  
pp. 7403-7407 ◽  
Author(s):  
Musti J. Swamy ◽  
Derek Marsh
Keyword(s):  
Lipid Bilayer ◽  
Protein Interactions ◽  
Spin Label ◽  
Lipid Bilayer Membranes ◽  
Bilayer Membranes ◽  
Lipid Protein Interactions

Real-Time Observation of Phospholipid Bilayer Membrane Restructuring Induced by Protein Molecules using Atomic Force Microscopy

2001 ◽  
Vol 7 (S2) ◽  
pp. 858-859
Author(s):  
Hong Xing You ◽  
Xiaoyang Qi ◽  
Lei Yu
Keyword(s):  
Atomic Force Microscopy ◽  
Real Time ◽  
Lipid Bilayer ◽  
Protein Interactions ◽  
Phospholipid Bilayer ◽  
Biological Processes ◽  
Lipid Bilayer Membranes ◽  
Bilayer Membranes ◽  
Force Microscopy ◽  
Atomic Force

Atomic force microscopy (AFM) allows the surfaces of native biological materials to be imaged in aqueous solution with submolecular resolution. The ability to perform AFM imaging in aqueous and physiological environment has made it possible to monitor important biological processes in real time at high resolution. Currently, there is a great deal of interest in AFM studies of the structure and property of lipid bilayer membranes and protein interactions with lipid bilayer membranes. Lipid bilayer membranes in biological cells form a permeability barrier, which controls the flow of ions, water, and other molecules between biological cells and their environments, whereas membrane-bound and/or membrane-associated proteins are responsible for most of the dynamic functions carried out by the membrane. However, real-time AFM monitoring of dynamic biological processes has been challenged by the limited temporal resolution of AFM, potential physical damage to soft biological samples, and intrinsic complexity of biological processes. There are few successful examples of AFM real-time studies of dynamic biological events, particularly in the aspect of protein interactions with lipid bilayer membranes.We have attempted to use atomic force microscopy to study interactions between a particular protein, saposin C, and phospholipid bilayer membranes in real time. Saposin C (Sap C), a small glycoprotein, is an essential co-factor for the hydrolysis of glucosylceramide by glucosylceramidase in lysosomes, and a deficiency of Sap C leads to a variant form of Gauchers’ diseases. Supported planar phospholipid bilayer membranes were used in the study.

Formation of asymmetrical planar lipid bilayer membranes from characterized monolayers

1983 ◽  
Vol 7 (4) ◽  
pp. 299-310 ◽  
Author(s):  
Pierre Tancrède ◽  
Paul Paquin ◽  
André Houle ◽  
Roger M. Leblanc
Keyword(s):  
Lipid Bilayer ◽  
Planar Lipid Bilayer ◽  
Lipid Bilayer Membranes ◽  
Bilayer Membranes

Calcium-induced reversible assembly of phosphorylated amphiphile within lipid bilayer membranes

2021 ◽  
Author(s):  
Yusuke Shimizu ◽  
Kohei Sato ◽  
Kazushi Kinbara
Keyword(s):  
Membrane Proteins ◽  
Lipid Bilayer ◽  
Phenylene Ethynylene ◽  
Lipid Bilayer Membranes ◽  
Bilayer Membranes

Inspired by calcium-induced reversible assembly and disassembly of membrane proteins found in nature, here we developed a phosphorylated amphiphile (PA) that contains an oligo(phenylene-ethynylene) unit as a hydrophobic unit and...

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