G-Protein-Mediated Signaling in Cholesterol-Enriched Arterial Smooth Muscle Cells. 2. Role of Protein Kinase C-δ in the Regulation of Eicosanoid Production†

Biochemistry ◽  
1997 ◽  
Vol 36 (31) ◽  
pp. 9532-9539 ◽  
Author(s):  
Kenneth B. Pomerantz ◽  
Harry M. Lander ◽  
Barbara Summers ◽  
David P. Hajjar
Keyword(s):  
Smooth Muscle ◽  
Protein Kinase C ◽  
Protein Kinase ◽  
Smooth Muscle Cells ◽  
G Protein ◽  
Muscle Cells ◽  
Kinase C ◽  
Eicosanoid Production ◽  
Arterial Smooth Muscle Cells

scholarly journals Minimally Modified LDL Upregulates Endothelin Type A Receptors in Rat Coronary Arterial Smooth Muscle Cells

2013 ◽  
Vol 2013 ◽  
pp. 1-11 ◽  
Author(s):  
Jie Li ◽  
Lei Cao ◽  
Cang-Bao Xu ◽  
Jun-Jie Wang ◽  
Yong-Xiao Cao
Keyword(s):  
Smooth Muscle ◽  
Protein Kinase C ◽  
Protein Kinase ◽  
Smooth Muscle Cells ◽  
Coronary Arteries ◽  
Muscle Cells ◽  
Type A ◽  
Receptor Mrna ◽  
Kinase C ◽  
Arterial Smooth Muscle Cells

Minimally modified low-density lipoprotein (mmLDL) is a risk factor for cardiovascular disease. The present study investigated the effects of mmLDL on the expression of endothelin type A () receptors in coronary arteries. Rat coronary arteries were organ-cultured for 24 h. The contractile responses were recorded using a myographic system. receptor mRNA and protein expressions were determined using real-time PCR and western blotting, respectively. The results showed that organ-culturing in the presence of mmLDL enhanced the arterial contractility mediated by the receptor in a concentration-dependent and time-dependent manner. Culturing with mmLDL (10 μg/mL) for 24 h shifted the concentration-contractile curves toward the left significantly with increased of from control of and significantly increased receptor mRNA and protein levels. Inhibition of the protein kinase C, extracellular signal-related kinases 1 and 2 (ERK1/2), or NF-κB activities significantly attenuated the effects of mmLDL. The c-Jun N-terminal kinase inhibitor or the p38 pathway inhibitor, however, had no such effects. The results indicate that mmLDL upregulates the receptors in rat coronary arterial smooth muscle cells mainlyviaactivating protein kinase C, ERK1/2, and the downstream transcriptional factor, NF-κB.

Role of atypical protein kinase C isozymes and NF-κB in IL-1β-induced expression of cyclooxygenase-2 in human myometrial smooth muscle cells

2006 ◽  
Vol 210 (3) ◽  
pp. 637-643 ◽  
Author(s):  
Sara V. Duggan ◽  
Tamsin Lindstrom ◽  
Teresa Iglesias ◽  
Phillip R. Bennett ◽  
Giovanni E. Mann ◽  
...  
Keyword(s):  
Smooth Muscle ◽  
Protein Kinase C ◽  
Protein Kinase ◽  
Smooth Muscle Cells ◽  
Muscle Cells ◽  
Atypical Protein Kinase C ◽  
Induced Expression ◽  
Atypical Protein Kinase ◽  
Kinase C
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