Heat Shock Protein 80 ofNeurospora crassa, a Cytosolic Molecular Chaperone of the Eukaryotic Stress 90 Family, Interacts Directly with Heat Shock Protein 70†

Biochemistry ◽  
1997 ◽  
Vol 36 (33) ◽  
pp. 10221-10229 ◽  
Author(s):  
D. G. Freitag ◽  
P. M. Ouimet ◽  
T. L. Girvitz ◽  
M. Kapoor
Keyword(s):  
Heat Shock ◽  
Heat Shock Protein ◽  
Molecular Chaperone ◽  
Heat Shock Protein 70 ◽  
Ofneurospora Crassa

scholarly journals Molecular chaperone heat shock protein 70 participates in the labile phase of the development of behavioural sensitization induced by a single morphine exposure in mice

2013 ◽  
Vol 16 (3) ◽  
pp. 647-659 ◽  
Author(s):  
Wang-Jun Qin ◽  
Yan-Ting Wang ◽  
Min Zhang ◽  
Rui-Ting Wen ◽  
Qing Liu ◽  
...  
Keyword(s):  
Protein Synthesis ◽  
Heat Shock ◽  
Heat Shock Protein ◽  
Molecular Chaperone ◽  
Heat Shock Protein 70 ◽  
De Novo ◽  
Behavioural Plasticity ◽  
Protein Synthesis Inhibitor ◽  
Morphine Injection ◽  
Behavioural Sensitization

Abstract De-novo protein synthesis is required in the development of behavioural sensitization. A prior screening test from our laboratory has implicated heat shock protein 70 (Hsp70) as one of the proteins required in this behavioural plasticity. Thus, this study was designed to extend our understanding of the role of Hsp70 in the development of behavioural sensitization induced by a single morphine exposure in mice. First, by employing transcription inhibitor actinomycin D (AD) and protein synthesis inhibitor cycloheximide (CHX), we identified a protein synthesis-dependent labile phase (within 4 h after the first morphine injection) in the development of behavioural sensitization to a single morphine exposure. Second, Hsp70 protein expression in the nucleus accumbens correlated positively with locomotor responses of sensitized mice and, more importantly, the expression of Hsp70 increased within 1 h after the first morphine injection. Third, AD and CHX both prevented expression of Hsp70 and disrupted the development of the single morphine induced behavioural sensitization, which further implied Hsp70 was highly associated with behavioural sensitization. Finally, the selective Hsp70 inhibitor pifithrin-µ (PES) i.c.v. injected in mice prevented the development of behavioural sensitization and, critically, this inhibitory effect occurred only when PES was given within 1 h after the first morphine injection, which was within the labile phase of the development period. Taken together, we draw the conclusion that Hsp70 is crucially involved in the labile phase of the development of behavioural sensitization induced by a single morphine exposure, probably functioning as a molecular chaperone.

Purification, crystallization and preliminary X-ray crystallographic studies of S. cerevisiae Hsp40 Sis1

1999 ◽  
Vol 55 (6) ◽  
pp. 1234-1236 ◽  
Author(s):  
Bingdong Sha ◽  
Douglas Cyr
Keyword(s):  
Heat Shock ◽  
Heat Shock Protein ◽  
Atpase Activity ◽  
Molecular Chaperone ◽  
Molecular Chaperones ◽  
Heat Shock Protein 70 ◽  
Peptide Binding ◽  
X Ray ◽  
Cellular Processes

Heat-shock protein 70 (Hsp70), one of the major molecular chaperones, has been shown to play a central role in many cellular processes. Heat-shock protein 40 (Hsp40) works as a co-chaperone for Hsp70. Hsp40, bound by unfolded polypeptide, can interact directly with Hsp70 to stimulate the ATPase activity of Hsp70. Hsp40 can also bind to unfolded polypeptides and prevent them from aggregating in vitro, thus acting as an independent molecular chaperone. The S. cerevisiae Hsp40 Sis1 C-terminal peptide-binding domain has been crystallized. The crystals diffract to 2.7 Å and belong to space group P41212 or P43212 with a = 73.63, c = 80.16 Å. The structure determination by the MAD method is under way.

Isolation of a Latimeria menadoensis heat shock protein 70 (Lmhsp70) that has all the features of an inducible gene and encodes a functional molecular chaperone

2009 ◽  
Vol 282 (2) ◽  
pp. 185-196 ◽  
Author(s):  
Keoagile W. Modisakeng ◽  
Meesbah Jiwaji ◽  
Eva-Rachele Pesce ◽  
Jacques Robert ◽  
Chris T. Amemiya ◽  
...  
Keyword(s):  
Heat Shock ◽  
Heat Shock Protein ◽  
Molecular Chaperone ◽  
Heat Shock Protein 70 ◽  
Inducible Gene

Ammonia aggravates emotional stress-induced gastric mucosal oxidative injury through the cancellation of heat shock protein 70 (HSP70)

2001 ◽  
Vol 120 (5) ◽  
pp. A152-A152
Author(s):  
H SUZUKI ◽  
S NAGAHASHI ◽  
M MIYAZAWA ◽  
M MORI ◽  
H NAGATA ◽  
...  
Keyword(s):  
Heat Shock ◽  
Heat Shock Protein ◽  
Emotional Stress ◽  
Heat Shock Protein 70 ◽  
Oxidative Injury
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