Myristoylated and Nonmyristoylated Pools of Sea Urchin Sperm Flagellar Creatine Kinase Exist Side-by-Side:  Myristoylation Is Necessary for Efficient Lipid Association†

Biochemistry ◽  
1997 ◽  
Vol 36 (23) ◽  
pp. 6993-7002 ◽  
Author(s):  
Andrew F. G. Quest ◽  
D. J. Harvey ◽  
R. A. J. McIlhinney
Keyword(s):  
Creatine Kinase ◽  
Sea Urchin ◽  
Sea Urchin Sperm

scholarly journals Activation of sea-urchin sperm motility is accompanied by an increase in the creatine kinase exchange flux

1997 ◽  
Vol 325 (2) ◽  
pp. 411-416 ◽  
Author(s):  
Ferdi A. van DORSTEN ◽  
Markus WYSS ◽  
Theo WALLIMANN ◽  
Klaas NICOLAY
Keyword(s):  
Creatine Kinase ◽  
Sea Urchin ◽  
Magnetization Transfer ◽  
Order Rate Constant ◽  
Phosphate Transport ◽  
High Energy ◽  
Sperm Activation ◽  
Kinetics Of ◽  
Sea Urchin Sperm ◽  
Exchange Flux

The kinetics of the creatine kinase (CK) reaction were studied in suspensions of quiescent and active, intact sea-urchin spermatozoa in artificial seawater, using 31P-NMR magnetization transfer. In inactive sperm, no CK-mediated exchange flux was detected, whereas in activated motile sperm, the forward pseudo-first-order rate constant was 0.13±0.04 s-1 at 10 °C, corresponding to a steady-state CK flux of 3.1±0.5 mM·s-1. Intracellular pH shifted from 6.6±0.1 to 7.6±0.1 upon activation. The phosphocreatine (PCr)/ATP and PCr/Pi ratios were only marginally reduced in activated sperm, whereas the estimated cytosolic free ADP concentration increased remarkably from 9 μM in quiescent, to 114 μM in activated spermatozoa. The elevation of CK flux upon sperm activation is discussed in the light of the proposition that in sea-urchin spermatozoa, which are fuelled entirely by oxidative phosphorylation, high-energy phosphate transport is mediated by a ‘CK/PCr shuttle‘.

Sea urchin sperm creatine kinase: The flagellar isozyme is a microtubule-associated protein

1988 ◽  
Vol 178 (2) ◽  
pp. 307-317 ◽  
Author(s):  
Robert M. Tombes ◽  
A. Farr ◽  
Bennett M. Shapiro
Keyword(s):  
Creatine Kinase ◽  
Sea Urchin ◽  
Sea Urchin Sperm

scholarly journals Purification of an acrosin-like enzyme from sea urchin sperm.

1980 ◽  
Vol 255 (10) ◽  
pp. 4814-4820
Author(s):  
A.E. Levine ◽  
K.A. Walsh
Keyword(s):  
Sea Urchin ◽  
Sea Urchin Sperm

scholarly journals Structure of sea urchin sperm chromatin core particle.

1980 ◽  
Vol 255 (22) ◽  
pp. 10702-10709
Author(s):  
R.T. Simpson ◽  
L.W. Bergman
Keyword(s):  
Sea Urchin ◽  
Sperm Chromatin ◽  
Core Particle ◽  
Sea Urchin Sperm
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