Solution NMR Study of the Structural Basis of the Bohr Effect in the Monomeric Hemoglobins fromChironomus thummi thummi†

Biochemistry ◽  
1997 ◽  
Vol 36 (7) ◽  
pp. 1689-1698 ◽  
Author(s):  
Wei Zhang ◽  
Klaus Gersonde ◽  
Gerd N. La Mar
Keyword(s):  
Bohr Effect ◽  
Solution Nmr ◽  
Structural Basis ◽  
Nmr Study

Expression and Solution NMR Study of Multi-site Phosphomimetic Mutant BCL-2 Protein

2019 ◽  
Vol 26 (6) ◽  
pp. 449-457
Author(s):  
Ting Song ◽  
Keke Cao ◽  
Yu dan Fan ◽  
Zhichao Zhang ◽  
Zong W. Guo ◽  
...  
Keyword(s):  
Structural Change ◽  
Structural Biology ◽  
Quantum Coherence ◽  
Structural Changes ◽  
Solution Nmr ◽  
Flexible Loop ◽  
Loop Region ◽  
Loop Domain ◽  
Nmr Study ◽  
Binding Groove

Background: The significance of multi-site phosphorylation of BCL-2 protein in the flexible loop domain remains controversial, in part due to the lack of structural biology studies of phosphorylated BCL-2. Objective: The purpose of the study is to explore the phosphorylation induced structural changes of BCL-2 protein. Methods: We constructed a phosphomietic mutant BCL-2(62-206) (t69e, s70e and s87e) (EEEBCL- 2-EK (62-206)), in which the BH4 domain and the part of loop region was truncated (residues 2-61) to enable a backbone resonance assignment. The phosphorylation-induced structural change was visualized by overlapping a well dispersed 15N-1H heteronuclear single quantum coherence (HSQC) NMR spectroscopy between EEE-BCL-2-EK (62-206) and BCL-2. Results: The EEE-BCL-2-EK (62-206) protein reproduced the biochemical and cellular activity of the native phosphorylated BCL-2 (pBCL-2), which was distinct from non-phosphorylated BCL-2 (npBCL-2) protein. Some residues in BH3 binding groove occurred chemical shift in the EEEBCL- 2-EK (62-206) spectrum, indicating that the phosphorylation in the loop region induces a structural change of active site. Conclusion: The phosphorylation of BCL-2 induced structural change in BH3 binding groove.

Solution NMR Study of Liquid Silk

2016 ◽  
Vol 72 (4) ◽  
pp. P-208-P-208
Author(s):  
YU SUZUKI
Keyword(s):  
Solution Nmr ◽  
Nmr Study

Deformation of methylenecyclopropane in solution: NMR study in the nematic phase at 240 and 400 MHz

1980 ◽  
Vol 14 (1) ◽  
pp. 55-57 ◽  
Author(s):  
J. Courtieu ◽  
Y. Gounelle ◽  
J. Jullien ◽  
G. Balavoine ◽  
M. Fan
Keyword(s):  
Nematic Phase ◽  
Solution Nmr ◽  
Nmr Study

Binding of Phosphonic Acids to CdSe Quantum Dots: A Solution NMR Study

2011 ◽  
Vol 2 (3) ◽  
pp. 145-152 ◽  
Author(s):  
Raquel Gomes ◽  
Antti Hassinen ◽  
Agnieszka Szczygiel ◽  
Qiang Zhao ◽  
André Vantomme ◽  
...  
Keyword(s):  
Quantum Dots ◽  
Solution Nmr ◽  
Cdse Quantum Dots ◽  
Phosphonic Acids ◽  
Nmr Study

Solution NMR Study of the Interaction Between NTF2 and Nucleoporin FxFG Repeats

2003 ◽  
Vol 333 (3) ◽  
pp. 587-603 ◽  
Author(s):  
Jonathan Morrison ◽  
Ji-Chun Yang ◽  
Murray Stewart ◽  
David Neuhaus
Keyword(s):  
Solution Nmr ◽  
Nmr Study

scholarly journals Structural Basis for TatA Oligomerization: An NMR Study of Escherichia coli TatA Dimeric Structure

PLoS ONE ◽  
2014 ◽  
Vol 9 (8) ◽  
pp. e103157 ◽  
Author(s):  
Yi Zhang ◽  
Yunfei Hu ◽  
Hongwei Li ◽  
Changwen Jin
Keyword(s):  
Escherichia Coli ◽  
Structural Basis ◽  
Dimeric Structure ◽  
Nmr Study
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