Purification and Characterization of an Extracellular Heme-Binding Protein, HasA, Involved in Heme Iron Acquisition†

Biochemistry ◽  
1997 ◽  
Vol 36 (23) ◽  
pp. 7050-7057 ◽  
Author(s):  
Nadia Izadi ◽  
Yann Henry ◽  
Jean Haladjian ◽  
Michel E. Goldberg ◽  
Cécile Wandersman ◽  
...  
Keyword(s):  
Binding Protein ◽  
Iron Acquisition ◽  
Heme Iron ◽  
Heme Binding ◽  
Purification And Characterization ◽  
Heme Binding Protein

scholarly journals Molecular Characterization of a Newly Identified Heme-binding Protein Induced during Differentiation of urine Erythroleukemia Cells

1998 ◽  
Vol 273 (47) ◽  
pp. 31388-31394 ◽  
Author(s):  
Shigeru Taketani ◽  
Yasushi Adachi ◽  
Hirao Kohno ◽  
Susumu Ikehara ◽  
Rikio Tokunaga ◽  
...  
Keyword(s):  
Molecular Characterization ◽  
Binding Protein ◽  
Heme Binding ◽  
Erythroleukemia Cells ◽  
Heme Binding Protein

Characterization of the Periplasmic Heme-Binding Protein ShuT from the Heme Uptake System ofShigella dysenteriae†

Biochemistry ◽  
2005 ◽  
Vol 44 (39) ◽  
pp. 13179-13191 ◽  
Author(s):  
Suntara Eakanunkul ◽  
Gudrun S. Lukat-Rodgers ◽  
Suganya Sumithran ◽  
Arundhati Ghosh ◽  
Kenton R. Rodgers ◽  
...  
Keyword(s):  
Binding Protein ◽  
Uptake System ◽  
Heme Binding ◽  
Heme Uptake ◽  
Heme Binding Protein

scholarly journals Characterization of Heme Ligation Properties of Rv0203, a Secreted Heme Binding Protein Involved inMycobacterium tuberculosisHeme Uptake

Biochemistry ◽  
2012 ◽  
Vol 51 (7) ◽  
pp. 1518-1531 ◽  
Author(s):  
Cedric P. Owens ◽  
Jing Du ◽  
John H. Dawson ◽  
Celia W. Goulding
Keyword(s):  
Binding Protein ◽  
Heme Binding ◽  
Heme Ligation ◽  
Heme Binding Protein

scholarly journals A heme-binding protein produced byHaemophilus haemolyticusinhibits non-typeableHaemophilus influenzae

2019 ◽  
Author(s):  
Roger D. Latham ◽  
Mario Torrado ◽  
Brianna Atto ◽  
James L. Walshe ◽  
Richard Wilson ◽  
...  
Keyword(s):  
Pathogenic Bacteria ◽  
Biochemical Characterization ◽  
Binding Protein ◽  
Bacterial Species ◽  
Opportunistic Pathogen ◽  
Heme Iron ◽  
Heme Binding ◽  
Therapeutic Uses ◽  
Wide Range ◽  
Heme Binding Protein

AbstractMany commensal bacteria and opportunistic pathogens scavenge heme from their environment. Pathogens and host are engaged in an arms race to control access to heme, but similar conflicts between bacterial species that might regulate pathogen colonisation are largely unknown. We show here that a commensal bacterium,Haemophilus haemolyticus, makes hemophilin, a heme-binding protein that not only allows the bacterium to effectively scavenge heme for its own growth, but also inhibits co-culture of the opportunistic pathogen, non-typeableHaemophilus influenzae(NTHi), by heme starvation. Knockout of the hemophilin gene abrogates the ability ofH. haemolyticusto inhibit NTHi and an x-ray crystal structure shows that hemophilin has a previously unreported heme-binding structure. The bound heme molecule is deeply buried and the heme iron atom is coordinated through a single histidine side chain. Biochemical characterization shows that this arrangement allows heme to be captured in the ferrous or ferric state, and with small ferrous or ferric heme-ligands bound, suggesting hemophilin could function over in a wide range of physiological conditions. Our data raise the possibility that competition for heme between commensal and pathogenic bacteria can influence bacterial colonisation, and therefore disease likelihood, and suggest that strains ofH. haemolyticusthat overproduce hemophilin might have therapeutic uses in reducing colonisation and subsequent opportunistic infection by NTHi.

Characterization of SiaA, a Streptococcal Heme-Binding Protein Associated with a Heme ABC Transport System†

Biochemistry ◽  
2008 ◽  
Vol 47 (8) ◽  
pp. 2678-2688 ◽  
Author(s):  
Brian R. Sook ◽  
Darci R. Block ◽  
Suganya Sumithran ◽  
Griselle E. Montañez ◽  
Kenton R. Rodgers ◽  
...  
Keyword(s):  
Transport System ◽  
Binding Protein ◽  
Heme Binding ◽  
Abc Transport ◽  
Heme Binding Protein
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